Copper in PDB 3awy: Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie M84L Mutant Soaked in A Cu(II)-Containing Solution For 80 Hr
Enzymatic activity of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie M84L Mutant Soaked in A Cu(II)-Containing Solution For 80 Hr
All present enzymatic activity of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie M84L Mutant Soaked in A Cu(II)-Containing Solution For 80 Hr:
1.14.18.1;
Protein crystallography data
The structure of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie M84L Mutant Soaked in A Cu(II)-Containing Solution For 80 Hr, PDB code: 3awy
was solved by
Y.Matoba,
M.Sugiyama,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
30.00 /
1.58
|
Space group
|
P 21 21 2
|
Cell size a, b, c (Å), α, β, γ (°)
|
64.690,
96.340,
54.650,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
19 /
24.2
|
Copper Binding Sites:
The binding sites of Copper atom in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie M84L Mutant Soaked in A Cu(II)-Containing Solution For 80 Hr
(pdb code 3awy). This binding sites where shown within
5.0 Angstroms radius around Copper atom.
In total 6 binding sites of Copper where determined in the
Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie M84L Mutant Soaked in A Cu(II)-Containing Solution For 80 Hr, PDB code: 3awy:
Jump to Copper binding site number:
1;
2;
3;
4;
5;
6;
Copper binding site 1 out
of 6 in 3awy
Go back to
Copper Binding Sites List in 3awy
Copper binding site 1 out
of 6 in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie M84L Mutant Soaked in A Cu(II)-Containing Solution For 80 Hr
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 1 of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie M84L Mutant Soaked in A Cu(II)-Containing Solution For 80 Hr within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Cu500
b:17.2
occ:0.45
|
CU
|
A:CU500
|
0.0
|
17.2
|
0.5
|
CU
|
A:CU500
|
1.0
|
21.2
|
0.5
|
NE2
|
A:HIS38
|
1.9
|
17.3
|
1.0
|
NE2
|
A:HIS63
|
1.9
|
15.7
|
1.0
|
NE2
|
A:HIS54
|
2.1
|
19.2
|
1.0
|
CD2
|
A:HIS38
|
2.7
|
17.0
|
1.0
|
O
|
A:HOH509
|
2.7
|
18.1
|
0.5
|
CE1
|
A:HIS63
|
2.7
|
12.4
|
1.0
|
O
|
B:HOH537
|
2.8
|
18.9
|
1.0
|
CE1
|
A:HIS54
|
2.9
|
18.5
|
1.0
|
CE1
|
A:HIS38
|
3.0
|
18.7
|
1.0
|
CD2
|
A:HIS63
|
3.1
|
14.0
|
1.0
|
CD2
|
A:HIS54
|
3.1
|
21.4
|
1.0
|
CG
|
A:HIS38
|
3.9
|
15.7
|
1.0
|
ND1
|
A:HIS63
|
3.9
|
12.7
|
1.0
|
ND1
|
A:HIS38
|
4.0
|
16.9
|
1.0
|
CU
|
A:CU501
|
4.1
|
15.2
|
1.0
|
ND1
|
A:HIS54
|
4.1
|
16.1
|
1.0
|
CG
|
A:HIS63
|
4.1
|
12.9
|
1.0
|
CG
|
A:HIS54
|
4.2
|
17.6
|
1.0
|
CZ
|
A:PHE212
|
4.2
|
12.2
|
1.0
|
NE2
|
A:HIS216
|
4.3
|
15.0
|
1.0
|
CZ3
|
A:TRP62
|
4.4
|
13.8
|
1.0
|
CE2
|
A:PHE212
|
4.5
|
10.7
|
1.0
|
OH
|
B:TYR98
|
4.5
|
17.8
|
1.0
|
CE1
|
A:HIS216
|
4.5
|
14.2
|
1.0
|
CD1
|
A:ILE42
|
4.9
|
23.8
|
1.0
|
O
|
A:GLY53
|
4.9
|
16.7
|
1.0
|
CE3
|
A:TRP62
|
4.9
|
13.2
|
1.0
|
|
Copper binding site 2 out
of 6 in 3awy
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Copper Binding Sites List in 3awy
Copper binding site 2 out
of 6 in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie M84L Mutant Soaked in A Cu(II)-Containing Solution For 80 Hr
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 2 of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie M84L Mutant Soaked in A Cu(II)-Containing Solution For 80 Hr within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Cu500
b:21.2
occ:0.47
|
CU
|
A:CU500
|
0.0
|
21.2
|
0.5
|
CU
|
A:CU500
|
1.0
|
17.2
|
0.5
|
O
|
B:HOH537
|
1.9
|
18.9
|
1.0
|
O
|
A:HOH509
|
1.9
|
18.1
|
0.5
|
NE2
|
A:HIS38
|
2.1
|
17.3
|
1.0
|
NE2
|
A:HIS54
|
2.3
|
19.2
|
1.0
|
NE2
|
A:HIS63
|
2.5
|
15.7
|
1.0
|
CE1
|
A:HIS38
|
2.9
|
18.7
|
1.0
|
CD2
|
A:HIS54
|
3.1
|
21.4
|
1.0
|
CU
|
A:CU501
|
3.2
|
15.2
|
1.0
|
CD2
|
A:HIS38
|
3.3
|
17.0
|
1.0
|
CE1
|
A:HIS63
|
3.3
|
12.4
|
1.0
|
CE1
|
A:HIS54
|
3.4
|
18.5
|
1.0
|
CD2
|
A:HIS63
|
3.6
|
14.0
|
1.0
|
OH
|
B:TYR98
|
3.7
|
17.8
|
1.0
|
NE2
|
A:HIS216
|
3.8
|
15.0
|
1.0
|
ND1
|
A:HIS38
|
4.2
|
16.9
|
1.0
|
CE1
|
A:HIS216
|
4.2
|
14.2
|
1.0
|
CE2
|
A:PHE212
|
4.3
|
10.7
|
1.0
|
CZ
|
A:PHE212
|
4.3
|
12.2
|
1.0
|
CG
|
A:HIS54
|
4.3
|
17.6
|
1.0
|
CG
|
A:HIS38
|
4.3
|
15.7
|
1.0
|
CE2
|
B:TYR98
|
4.3
|
15.9
|
1.0
|
CD1
|
A:ILE42
|
4.4
|
23.8
|
1.0
|
ND1
|
A:HIS54
|
4.4
|
16.1
|
1.0
|
ND1
|
A:HIS63
|
4.5
|
12.7
|
1.0
|
CZ
|
B:TYR98
|
4.5
|
15.5
|
1.0
|
NE2
|
A:HIS190
|
4.5
|
13.6
|
1.0
|
CG
|
A:HIS63
|
4.7
|
12.9
|
1.0
|
NE2
|
A:HIS194
|
4.7
|
13.9
|
1.0
|
CD2
|
A:HIS216
|
4.8
|
13.0
|
1.0
|
CE1
|
A:PHE59
|
4.9
|
12.9
|
1.0
|
CE1
|
A:HIS190
|
5.0
|
15.9
|
1.0
|
|
Copper binding site 3 out
of 6 in 3awy
Go back to
Copper Binding Sites List in 3awy
Copper binding site 3 out
of 6 in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie M84L Mutant Soaked in A Cu(II)-Containing Solution For 80 Hr
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 3 of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie M84L Mutant Soaked in A Cu(II)-Containing Solution For 80 Hr within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Cu501
b:15.2
occ:0.97
|
O
|
B:HOH537
|
2.0
|
18.9
|
1.0
|
NE2
|
A:HIS190
|
2.0
|
13.6
|
1.0
|
NE2
|
A:HIS216
|
2.1
|
15.0
|
1.0
|
NE2
|
A:HIS194
|
2.1
|
13.9
|
1.0
|
O
|
A:HOH509
|
2.3
|
18.1
|
0.5
|
CE1
|
A:HIS216
|
2.9
|
14.2
|
1.0
|
CE1
|
A:HIS190
|
3.0
|
15.9
|
1.0
|
CD2
|
A:HIS190
|
3.0
|
12.9
|
1.0
|
CE1
|
A:HIS194
|
3.0
|
12.8
|
1.0
|
CD2
|
A:HIS194
|
3.1
|
12.3
|
1.0
|
CU
|
A:CU500
|
3.2
|
21.2
|
0.5
|
CD2
|
A:HIS216
|
3.2
|
13.0
|
1.0
|
CE2
|
B:TYR98
|
3.8
|
15.9
|
1.0
|
OH
|
B:TYR98
|
3.9
|
17.8
|
1.0
|
CU
|
A:CU500
|
4.1
|
17.2
|
0.5
|
ND1
|
A:HIS190
|
4.1
|
12.9
|
1.0
|
ND1
|
A:HIS216
|
4.1
|
13.5
|
1.0
|
CZ
|
B:TYR98
|
4.1
|
15.5
|
1.0
|
CG
|
A:HIS190
|
4.1
|
13.5
|
1.0
|
ND1
|
A:HIS194
|
4.2
|
12.1
|
1.0
|
CG
|
A:HIS194
|
4.2
|
12.4
|
1.0
|
CG
|
A:HIS216
|
4.2
|
12.2
|
1.0
|
CE2
|
A:PHE212
|
4.3
|
10.7
|
1.0
|
NE2
|
A:HIS63
|
4.4
|
15.7
|
1.0
|
CD2
|
A:HIS215
|
4.6
|
15.0
|
1.0
|
CD2
|
B:TYR98
|
4.7
|
13.5
|
1.0
|
NE2
|
A:HIS215
|
4.7
|
12.1
|
1.0
|
CE1
|
A:PHE59
|
4.8
|
12.9
|
1.0
|
NE2
|
A:HIS38
|
4.8
|
17.3
|
1.0
|
CD2
|
A:HIS63
|
4.8
|
14.0
|
1.0
|
CZ
|
A:PHE212
|
4.9
|
12.2
|
1.0
|
|
Copper binding site 4 out
of 6 in 3awy
Go back to
Copper Binding Sites List in 3awy
Copper binding site 4 out
of 6 in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie M84L Mutant Soaked in A Cu(II)-Containing Solution For 80 Hr
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 4 of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie M84L Mutant Soaked in A Cu(II)-Containing Solution For 80 Hr within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Cu503
b:42.0
occ:0.50
|
NE2
|
A:HIS277
|
1.9
|
38.4
|
1.0
|
NE2
|
A:HIS279
|
2.6
|
84.8
|
1.0
|
O
|
A:HOH663
|
2.6
|
32.9
|
0.5
|
CE1
|
A:HIS277
|
2.8
|
38.3
|
1.0
|
CD2
|
A:HIS277
|
3.0
|
41.2
|
1.0
|
CD2
|
A:HIS279
|
3.3
|
85.1
|
1.0
|
CE1
|
A:HIS279
|
3.7
|
84.5
|
1.0
|
ND1
|
A:HIS277
|
4.0
|
39.9
|
1.0
|
CG
|
A:HIS277
|
4.1
|
43.6
|
1.0
|
CG
|
A:HIS279
|
4.6
|
85.2
|
1.0
|
ND1
|
A:HIS279
|
4.7
|
84.6
|
1.0
|
CG
|
A:PRO231
|
4.7
|
29.9
|
1.0
|
|
Copper binding site 5 out
of 6 in 3awy
Go back to
Copper Binding Sites List in 3awy
Copper binding site 5 out
of 6 in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie M84L Mutant Soaked in A Cu(II)-Containing Solution For 80 Hr
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 5 of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie M84L Mutant Soaked in A Cu(II)-Containing Solution For 80 Hr within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Cu504
b:64.0
occ:0.51
|
NE2
|
A:HIS180
|
2.0
|
20.4
|
1.0
|
CD2
|
A:HIS180
|
3.0
|
18.5
|
1.0
|
CE1
|
A:HIS180
|
3.0
|
21.3
|
1.0
|
O
|
A:HOH639
|
3.7
|
29.0
|
1.0
|
CA
|
A:GLY186
|
3.9
|
17.3
|
1.0
|
OE2
|
A:GLU175
|
3.9
|
43.7
|
1.0
|
ND1
|
A:HIS180
|
4.1
|
20.8
|
1.0
|
CG
|
A:HIS180
|
4.1
|
16.9
|
1.0
|
N
|
A:GLY186
|
4.3
|
19.5
|
1.0
|
O
|
A:HOH756
|
4.3
|
46.0
|
1.0
|
OE1
|
A:GLU175
|
4.5
|
49.6
|
1.0
|
CD
|
A:GLU175
|
4.6
|
37.3
|
1.0
|
C
|
A:ARG185
|
4.8
|
19.3
|
1.0
|
|
Copper binding site 6 out
of 6 in 3awy
Go back to
Copper Binding Sites List in 3awy
Copper binding site 6 out
of 6 in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie M84L Mutant Soaked in A Cu(II)-Containing Solution For 80 Hr
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 6 of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie M84L Mutant Soaked in A Cu(II)-Containing Solution For 80 Hr within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Cu502
b:46.6
occ:0.80
|
NE2
|
B:HIS82
|
2.1
|
30.4
|
1.0
|
CD2
|
B:HIS82
|
2.8
|
27.1
|
1.0
|
CE1
|
B:HIS82
|
3.2
|
27.2
|
1.0
|
O
|
A:HOH760
|
3.6
|
50.5
|
1.0
|
CG
|
B:HIS82
|
4.0
|
25.5
|
1.0
|
ND1
|
B:HIS82
|
4.2
|
24.7
|
1.0
|
O
|
A:MET43
|
4.2
|
28.8
|
1.0
|
|
Reference:
Y.Matoba,
N.Bando,
K.Oda,
M.Noda,
F.Higashikawa,
T.Kumagai,
M.Sugiyama.
A Molecular Mechanism For Copper Transportation to Tyrosinase That Is Assisted By A Metallochaperone, Caddie Protein J.Biol.Chem. V. 286 30219 2011.
ISSN: ISSN 0021-9258
PubMed: 21730070
DOI: 10.1074/JBC.M111.256818
Page generated: Wed Jul 31 00:42:19 2024
|