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Copper in PDB 3awu: Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 40 H

Enzymatic activity of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 40 H

All present enzymatic activity of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 40 H:
1.14.18.1;

Protein crystallography data

The structure of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 40 H, PDB code: 3awu was solved by Y.Matoba, M.Sugiyama, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 30.00 / 1.16
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 65.100, 97.790, 54.990, 90.00, 90.00, 90.00
R / Rfree (%) 13.8 / 17.5

Copper Binding Sites:

The binding sites of Copper atom in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 40 H (pdb code 3awu). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 6 binding sites of Copper where determined in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 40 H, PDB code: 3awu:
Jump to Copper binding site number: 1; 2; 3; 4; 5; 6;

Copper binding site 1 out of 6 in 3awu

Go back to Copper Binding Sites List in 3awu
Copper binding site 1 out of 6 in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 40 H


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 40 H within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu500

b:12.1
occ:0.26
 CU A:CU500 0.0 12.1 0.3
CU A:CU500 1.2 13.9 0.4
NE2 A:HIS38 1.8 20.0 1.0
NE2 A:HIS63 1.9 12.8 1.0
NE2 A:HIS54 2.2 13.8 0.7
CD2 A:HIS38 2.6 18.3 1.0
CE1 A:HIS63 2.7 11.5 1.0
O B:HOH580 2.8 18.6 1.0
CE1 A:HIS38 2.8 19.6 1.0
CE1 A:HIS54 2.9 13.2 0.7
O A:HOH510 3.2 26.5 0.9
CD2 A:HIS63 3.2 11.7 1.0
CD2 A:HIS54 3.3 13.6 0.7
CG A:HIS38 3.8 15.3 1.0
ND1 A:HIS38 3.8 17.1 1.0
ND1 A:HIS63 3.9 10.2 1.0
ND1 A:HIS54 4.1 12.2 0.7
CZ A:PHE212 4.2 10.0 1.0
CG A:HIS63 4.2 9.4 1.0
CU A:CU501 4.2 14.8 0.8
CG A:HIS54 4.3 11.3 0.7
NE2 A:HIS216 4.4 11.3 1.0
CZ3 A:TRP62 4.4 12.0 1.0
CE2 A:PHE212 4.4 10.8 1.0
CE1 A:HIS216 4.6 10.7 1.0
OH B:TYR98 4.7 20.9 1.0
CD1 A:ILE42 4.8 34.0 1.0
O A:GLY53 4.9 11.5 1.0
CE3 A:TRP62 4.9 10.6 1.0

Copper binding site 2 out of 6 in 3awu

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Copper binding site 2 out of 6 in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 40 H


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 40 H within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu500

b:13.9
occ:0.37
 CU A:CU500 0.0 13.9 0.4
CU A:CU500 1.2 12.1 0.3
O B:HOH580 1.9 18.6 1.0
NE2 A:HIS54 2.0 13.8 0.7
NE2 A:HIS38 2.2 20.0 1.0
O A:HOH510 2.2 26.5 0.9
CE1 A:HIS38 2.7 19.6 1.0
NE2 A:HIS63 2.7 12.8 1.0
CD2 A:HIS54 2.9 13.6 0.7
CE1 A:HIS54 3.1 13.2 0.7
CE1 A:HIS63 3.3 11.5 1.0
CU A:CU501 3.4 14.8 0.8
CD2 A:HIS38 3.4 18.3 1.0
OH B:TYR98 3.7 20.9 1.0
CD2 A:HIS63 4.0 11.7 1.0
ND1 A:HIS38 4.0 17.1 1.0
CD1 A:ILE42 4.1 34.0 1.0
CG A:HIS54 4.1 11.3 0.7
NE2 A:HIS216 4.1 11.3 1.0
ND1 A:HIS54 4.2 12.2 0.7
CG A:HIS38 4.3 15.3 1.0
CE2 A:PHE212 4.4 10.8 1.0
CE1 A:HIS216 4.5 10.7 1.0
CZ A:PHE212 4.5 10.0 1.0
ND1 A:HIS63 4.6 10.2 1.0
CE2 B:TYR98 4.6 16.8 1.0
CZ B:TYR98 4.6 16.6 1.0
NE2 A:HIS190 4.7 10.4 1.0
CG1 A:ILE42 4.8 24.5 1.0
O B:HOH513 4.9 23.5 0.7
NE2 A:HIS194 4.9 9.1 1.0
CB A:HIS54 4.9 9.1 0.3
CG A:HIS63 4.9 9.4 1.0
CE1 A:PHE59 4.9 9.9 1.0

Copper binding site 3 out of 6 in 3awu

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Copper binding site 3 out of 6 in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 40 H


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 40 H within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu501

b:14.8
occ:0.78
 O B:HOH580 1.9 18.6 1.0
NE2 A:HIS190 2.0 10.4 1.0
NE2 A:HIS194 2.1 9.1 1.0
NE2 A:HIS216 2.1 11.3 1.0
O A:HOH510 2.5 26.5 0.9
CE1 A:HIS190 3.0 10.2 1.0
CE1 A:HIS216 3.0 10.7 1.0
CE1 A:HIS194 3.0 9.0 1.0
CD2 A:HIS190 3.1 9.5 1.0
CD2 A:HIS194 3.1 8.7 1.0
CD2 A:HIS216 3.3 11.4 1.0
CU A:CU500 3.4 13.9 0.4
OH B:TYR98 4.0 20.9 1.0
CE2 B:TYR98 4.0 16.8 1.0
ND1 A:HIS190 4.1 10.1 1.0
CG A:HIS190 4.2 9.1 1.0
ND1 A:HIS216 4.2 10.0 1.0
ND1 A:HIS194 4.2 8.8 1.0
CE2 A:PHE212 4.2 10.8 1.0
CG A:HIS194 4.2 8.6 1.0
CU A:CU500 4.2 12.1 0.3
CZ B:TYR98 4.2 16.6 1.0
CG A:HIS216 4.3 9.4 1.0
NE2 A:HIS63 4.5 12.8 1.0
CD2 A:HIS215 4.6 11.3 1.0
NE2 A:HIS215 4.7 12.2 1.0
CE1 A:PHE59 4.8 9.9 1.0
CZ A:PHE212 4.8 10.0 1.0
NE2 A:HIS38 4.9 20.0 1.0
CD2 A:PHE212 4.9 9.4 1.0
CD2 B:TYR98 4.9 15.1 1.0

Copper binding site 4 out of 6 in 3awu

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Copper binding site 4 out of 6 in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 40 H


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 40 H within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu503

b:25.2
occ:0.50
 NE2 A:HIS277 1.9 23.9 1.0
NE2 A:HIS279 2.0 45.8 1.0
O A:HOH675 2.6 26.8 0.5
CE1 A:HIS279 2.9 45.9 1.0
CE1 A:HIS277 2.9 24.9 1.0
CD2 A:HIS277 3.0 25.8 1.0
CD2 A:HIS279 3.1 47.5 1.0
ND1 A:HIS279 4.0 47.4 1.0
ND1 A:HIS277 4.0 25.8 1.0
CG A:HIS277 4.1 27.1 1.0
CG A:HIS279 4.2 48.6 1.0
O A:HOH714 4.7 28.4 1.0
CG A:PRO231 4.7 21.6 1.0

Copper binding site 5 out of 6 in 3awu

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Copper binding site 5 out of 6 in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 40 H


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 5 of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 40 H within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu502

b:18.2
occ:0.71
 OE1 B:GLU67 1.6 33.0 1.0
NE2 B:HIS82 2.0 15.9 0.8
O B:HIS68 2.0 29.7 1.0
ND1 B:HIS68 2.0 23.8 1.0
N B:HIS68 2.7 26.6 1.0
CD B:GLU67 2.7 32.1 1.0
C B:HIS68 2.8 30.5 1.0
CE1 B:HIS68 2.9 23.6 1.0
CD2 B:HIS82 2.9 15.8 0.8
CE1 B:HIS82 3.0 14.9 0.8
CA B:HIS68 3.1 26.2 1.0
CG B:HIS68 3.1 23.6 1.0
C B:GLU67 3.2 27.2 1.0
CE1 B:HIS82 3.3 14.6 0.2
ND1 B:HIS82 3.3 14.9 0.2
CB B:GLU67 3.4 29.0 1.0
CB B:HIS68 3.6 24.5 1.0
CG B:GLU67 3.6 30.7 1.0
OE2 B:GLU67 3.7 33.5 1.0
CA B:GLU67 3.9 25.4 1.0
O B:GLU67 3.9 30.3 1.0
N B:GLY69 3.9 34.5 1.0
N B:GLY70 4.0 42.8 1.0
NE2 B:HIS68 4.1 22.2 1.0
ND1 B:HIS82 4.1 14.0 0.8
CG B:HIS82 4.1 14.6 0.8
O A:MET43 4.2 17.2 1.0
CD2 B:HIS68 4.2 22.0 1.0
CA B:GLY69 4.5 37.9 1.0
NE2 B:HIS82 4.6 14.2 0.2
CG B:HIS82 4.7 13.9 0.2
CA B:GLY70 4.7 41.6 1.0
C B:GLY69 4.8 42.4 1.0
O B:HOH608 4.8 20.6 1.0

Copper binding site 6 out of 6 in 3awu

Go back to Copper Binding Sites List in 3awu
Copper binding site 6 out of 6 in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 40 H


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 6 of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 40 H within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu502

b:23.2
occ:0.20
 SD B:MET84 1.5 26.1 0.4
CG B:MET84 2.0 17.3 0.4
CE B:MET84 2.2 19.9 0.6
CE B:MET84 2.3 23.6 0.4
NE2 B:HIS82 2.5 14.2 0.2
SD B:MET84 2.7 23.1 0.6
N B:NO3504 2.8 21.9 0.6
O1 B:NO3504 2.9 29.2 0.6
O3 B:NO3504 3.0 23.1 0.6
CB B:MET84 3.1 15.7 0.4
CB B:MET84 3.1 15.4 0.6
CE1 B:HIS97 3.2 16.1 0.3
NE2 B:HIS97 3.3 16.5 0.3
CE1 B:HIS82 3.4 14.6 0.2
O2 B:NO3504 3.4 16.2 0.6
CG B:MET84 3.4 18.3 0.6
O A:ILE42 3.4 14.9 1.0
CD2 B:HIS82 3.5 13.7 0.2
CE1 B:HIS82 3.6 14.9 0.8
O A:MET43 3.7 17.2 1.0
CA A:MET43 3.8 14.6 1.0
O A:HOH511 3.8 35.6 1.0
ND1 B:HIS82 3.8 14.0 0.8
CE1 B:HIS97 3.9 16.3 0.7
C A:MET43 4.0 14.6 1.0
ND1 B:HIS97 4.3 15.6 0.7
ND1 B:HIS97 4.4 15.5 0.3
CA B:MET84 4.4 13.2 1.0
C A:ILE42 4.4 14.7 1.0
ND1 B:HIS82 4.5 14.9 0.2
CD2 B:HIS97 4.5 16.8 0.3
N A:MET43 4.6 13.3 1.0
CG B:HIS82 4.6 13.9 0.2
N B:MET84 4.7 12.6 1.0
CB A:MET43 4.7 16.5 1.0
NE2 B:HIS82 4.9 15.9 0.8
C B:VAL83 4.9 11.5 1.0
CG1 B:ILE92 5.0 14.7 1.0
O A:ASP45 5.0 20.8 1.0
CD1 B:ILE92 5.0 20.8 1.0
ND1 B:HIS68 5.0 23.8 1.0

Reference:

Y.Matoba, N.Bando, K.Oda, M.Noda, F.Higashikawa, T.Kumagai, M.Sugiyama. A Molecular Mechanism For Copper Transportation to Tyrosinase That Is Assisted By A Metallochaperone, Caddie Protein J.Biol.Chem. V. 286 30219 2011.
ISSN: ISSN 0021-9258
PubMed: 21730070
DOI: 10.1074/JBC.M111.256818
Page generated: Wed Jul 31 00:42:19 2024

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