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Copper in PDB 3awt: Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 20 Hr: Occupancy of Cu(II) Is High

Enzymatic activity of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 20 Hr: Occupancy of Cu(II) Is High

All present enzymatic activity of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 20 Hr: Occupancy of Cu(II) Is High:
1.14.18.1;

Protein crystallography data

The structure of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 20 Hr: Occupancy of Cu(II) Is High, PDB code: 3awt was solved by Y.Matoba, M.Sugiyama, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 30.00 / 1.35
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 65.150, 98.060, 55.090, 90.00, 90.00, 90.00
R / Rfree (%) 17.5 / 20.9

Copper Binding Sites:

The binding sites of Copper atom in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 20 Hr: Occupancy of Cu(II) Is High (pdb code 3awt). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 5 binding sites of Copper where determined in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 20 Hr: Occupancy of Cu(II) Is High, PDB code: 3awt:
Jump to Copper binding site number: 1; 2; 3; 4; 5;

Copper binding site 1 out of 5 in 3awt

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Copper binding site 1 out of 5 in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 20 Hr: Occupancy of Cu(II) Is High


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 20 Hr: Occupancy of Cu(II) Is High within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu500

b:19.2
occ:0.34
 O B:HOH624 2.0 21.1 1.0
NE2 A:HIS54 2.0 13.8 0.4
O A:HOH511 2.2 25.2 0.8
NE2 A:HIS38 2.2 16.1 1.0
CE1 A:HIS38 2.4 13.0 1.0
NE2 A:HIS63 2.8 10.1 1.0
CD2 A:HIS54 2.9 14.4 0.4
CE1 A:HIS54 3.1 12.9 0.4
CE1 A:HIS63 3.2 8.3 1.0
CU A:CU501 3.4 15.0 0.6
CD2 A:HIS38 3.6 16.1 1.0
OH B:TYR98 3.7 26.4 1.0
ND1 A:HIS38 3.7 13.5 1.0
CD2 A:HIS63 4.0 9.1 1.0
CD1 A:ILE42 4.0 45.9 1.0
NE2 A:HIS216 4.1 12.1 1.0
CG A:HIS54 4.1 12.0 0.4
ND1 A:HIS54 4.2 12.5 0.4
CG A:HIS38 4.3 12.8 1.0
CE2 A:PHE212 4.4 10.1 1.0
CE1 A:HIS216 4.4 13.8 1.0
CZ A:PHE212 4.5 9.5 1.0
ND1 A:HIS63 4.5 8.8 1.0
NE2 A:HIS190 4.7 11.8 1.0
CZ B:TYR98 4.9 17.9 1.0
O B:HOH512 4.9 12.3 0.6
CG A:HIS63 4.9 9.1 1.0
CE1 A:PHE59 4.9 8.9 1.0
CB A:HIS54 5.0 10.3 0.6

Copper binding site 2 out of 5 in 3awt

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Copper binding site 2 out of 5 in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 20 Hr: Occupancy of Cu(II) Is High


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 20 Hr: Occupancy of Cu(II) Is High within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu501

b:15.0
occ:0.55
 O B:HOH624 1.8 21.1 1.0
NE2 A:HIS190 2.0 11.8 1.0
NE2 A:HIS194 2.2 10.0 1.0
NE2 A:HIS216 2.3 12.1 1.0
O A:HOH511 2.7 25.2 0.8
CE1 A:HIS190 2.9 10.8 1.0
CE1 A:HIS216 3.0 13.8 1.0
CD2 A:HIS190 3.0 10.3 1.0
CE1 A:HIS194 3.1 9.2 1.0
CD2 A:HIS194 3.1 10.4 1.0
CU A:CU500 3.4 19.2 0.3
CD2 A:HIS216 3.4 13.0 1.0
OH B:TYR98 3.9 26.4 1.0
ND1 A:HIS190 4.0 9.7 1.0
CG A:HIS190 4.1 9.4 1.0
CE2 B:TYR98 4.2 16.0 1.0
ND1 A:HIS194 4.2 8.4 1.0
ND1 A:HIS216 4.2 10.8 1.0
CG A:HIS194 4.3 9.3 1.0
CE2 A:PHE212 4.3 10.1 1.0
CZ B:TYR98 4.3 17.9 1.0
CG A:HIS216 4.4 10.6 1.0
CD2 A:HIS215 4.6 13.9 1.0
NE2 A:HIS63 4.6 10.1 1.0
NE2 A:HIS215 4.6 14.6 1.0
CE1 A:PHE59 4.7 8.9 1.0
CZ A:PHE212 4.9 9.5 1.0
CE1 A:HIS38 5.0 13.0 1.0
CD2 A:PHE212 5.0 7.2 1.0

Copper binding site 3 out of 5 in 3awt

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Copper binding site 3 out of 5 in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 20 Hr: Occupancy of Cu(II) Is High


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 20 Hr: Occupancy of Cu(II) Is High within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu503

b:24.0
occ:0.50
 NE2 A:HIS277 2.0 26.8 1.0
NE2 A:HIS279 2.2 41.1 1.0
CD2 A:HIS277 3.0 30.0 1.0
CE1 A:HIS277 3.0 24.7 1.0
CD2 A:HIS279 3.1 43.5 1.0
CE1 A:HIS279 3.3 42.6 1.0
ND1 A:HIS277 4.1 25.8 1.0
CG A:HIS277 4.1 29.5 1.0
CG A:HIS279 4.3 45.5 1.0
ND1 A:HIS279 4.4 43.2 1.0
O A:HOH779 4.5 30.7 1.0
CG A:PRO231 4.7 22.2 1.0

Copper binding site 4 out of 5 in 3awt

Go back to Copper Binding Sites List in 3awt
Copper binding site 4 out of 5 in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 20 Hr: Occupancy of Cu(II) Is High


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 20 Hr: Occupancy of Cu(II) Is High within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu502

b:18.9
occ:0.82
 OE1 B:GLU67 1.7 29.7 1.0
NE2 B:HIS82 2.0 22.0 1.0
ND1 B:HIS68 2.0 22.5 1.0
O B:HIS68 2.1 28.8 1.0
N B:HIS68 2.6 24.6 1.0
O B:HOH851 2.8 37.0 1.0
C B:HIS68 2.9 30.5 1.0
CE1 B:HIS68 2.9 23.2 1.0
CD B:GLU67 2.9 33.6 1.0
CD2 B:HIS82 3.0 19.8 1.0
CE1 B:HIS82 3.0 18.9 1.0
CG B:HIS68 3.1 22.8 1.0
CA B:HIS68 3.1 24.1 1.0
C B:GLU67 3.4 25.5 1.0
CB B:GLU67 3.4 24.6 1.0
CB B:HIS68 3.5 22.7 1.0
CG B:GLU67 3.7 31.9 1.0
OE2 B:GLU67 3.9 31.5 1.0
CA B:GLU67 4.0 25.0 1.0
NE2 B:HIS68 4.0 23.5 1.0
N B:GLY69 4.1 38.4 1.0
ND1 B:HIS82 4.1 17.4 1.0
CD2 B:HIS68 4.1 20.8 1.0
CG B:HIS82 4.1 16.9 1.0
O B:GLU67 4.2 28.7 1.0
O A:MET43 4.2 16.8 1.0
N B:GLY70 4.4 49.7 1.0
O B:HOH597 4.7 19.2 1.0
CA B:GLY69 4.7 45.1 1.0
C B:GLY69 4.9 50.6 1.0

Copper binding site 5 out of 5 in 3awt

Go back to Copper Binding Sites List in 3awt
Copper binding site 5 out of 5 in the Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 20 Hr: Occupancy of Cu(II) Is High


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 5 of Crystal Structure of Streptomyces Tyrosinase in A Complex with Caddie Soaked in A Cu(II)-Containing Solution For 20 Hr: Occupancy of Cu(II) Is High within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu502

b:18.9
occ:0.17
 SD B:MET84 1.4 47.2 0.4
CE B:MET84 1.8 44.4 0.6
CE B:MET84 2.1 26.4 0.4
CG B:MET84 2.2 18.6 0.4
SD B:MET84 2.7 27.1 0.6
N B:NO3509 3.0 24.9 0.8
CB B:MET84 3.0 16.8 0.6
O1 B:NO3509 3.0 32.4 0.8
CB B:MET84 3.0 16.6 0.4
O3 B:NO3509 3.2 20.4 0.8
O A:HOH510 3.4 25.4 0.8
CG B:MET84 3.4 17.5 0.6
O2 B:NO3509 3.5 15.3 0.8
O A:ILE42 3.5 16.4 1.0
O A:MET43 3.7 16.8 1.0
CE1 B:HIS82 3.7 18.9 1.0
CE1 B:HIS97 3.9 17.1 1.0
CA A:MET43 3.9 15.4 1.0
ND1 B:HIS82 4.0 17.4 1.0
C A:MET43 4.1 16.3 1.0
ND1 B:HIS97 4.4 17.0 1.0
CA B:MET84 4.4 12.8 1.0
C A:ILE42 4.5 15.7 1.0
CD1 B:ILE92 4.7 16.6 1.0
O A:ASP45 4.7 17.5 1.0
N B:MET84 4.7 12.6 1.0
N A:MET43 4.8 15.3 1.0
CG1 B:ILE92 4.9 12.0 1.0
CB A:MET43 4.9 15.9 1.0
ND1 B:HIS68 5.0 22.5 1.0
NE2 B:HIS82 5.0 22.0 1.0
C B:VAL83 5.0 13.4 1.0

Reference:

Y.Matoba, N.Bando, K.Oda, M.Noda, F.Higashikawa, T.Kumagai, M.Sugiyama. A Molecular Mechanism For Copper Transportation to Tyrosinase That Is Assisted By A Metallochaperone, Caddie Protein J.Biol.Chem. V. 286 30219 2011.
ISSN: ISSN 0021-9258
PubMed: 21730070
DOI: 10.1074/JBC.M111.256818
Page generated: Wed Jul 31 00:42:19 2024

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