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Copper in PDB 2zwe: Crystal Structure of the Copper-Bound Tyrosinase in Complex with A Caddie Protein From Streptomyces Castaneoglobisporus Obtained By Soaking the Deoxy-Form Crystal in Dioxygen-Saturated Solution For 40 Minutes

Enzymatic activity of Crystal Structure of the Copper-Bound Tyrosinase in Complex with A Caddie Protein From Streptomyces Castaneoglobisporus Obtained By Soaking the Deoxy-Form Crystal in Dioxygen-Saturated Solution For 40 Minutes

All present enzymatic activity of Crystal Structure of the Copper-Bound Tyrosinase in Complex with A Caddie Protein From Streptomyces Castaneoglobisporus Obtained By Soaking the Deoxy-Form Crystal in Dioxygen-Saturated Solution For 40 Minutes:
1.14.18.1;

Protein crystallography data

The structure of Crystal Structure of the Copper-Bound Tyrosinase in Complex with A Caddie Protein From Streptomyces Castaneoglobisporus Obtained By Soaking the Deoxy-Form Crystal in Dioxygen-Saturated Solution For 40 Minutes, PDB code: 2zwe was solved by Y.Matoba, M.Sugiyama, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 30.00 / 1.32
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 65.240, 97.880, 55.090, 90.00, 90.00, 90.00
R / Rfree (%) 17.4 / 20.8

Copper Binding Sites:

The binding sites of Copper atom in the Crystal Structure of the Copper-Bound Tyrosinase in Complex with A Caddie Protein From Streptomyces Castaneoglobisporus Obtained By Soaking the Deoxy-Form Crystal in Dioxygen-Saturated Solution For 40 Minutes (pdb code 2zwe). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 6 binding sites of Copper where determined in the Crystal Structure of the Copper-Bound Tyrosinase in Complex with A Caddie Protein From Streptomyces Castaneoglobisporus Obtained By Soaking the Deoxy-Form Crystal in Dioxygen-Saturated Solution For 40 Minutes, PDB code: 2zwe:
Jump to Copper binding site number: 1; 2; 3; 4; 5; 6;

Copper binding site 1 out of 6 in 2zwe

Go back to Copper Binding Sites List in 2zwe
Copper binding site 1 out of 6 in the Crystal Structure of the Copper-Bound Tyrosinase in Complex with A Caddie Protein From Streptomyces Castaneoglobisporus Obtained By Soaking the Deoxy-Form Crystal in Dioxygen-Saturated Solution For 40 Minutes


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure of the Copper-Bound Tyrosinase in Complex with A Caddie Protein From Streptomyces Castaneoglobisporus Obtained By Soaking the Deoxy-Form Crystal in Dioxygen-Saturated Solution For 40 Minutes within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu501

b:30.3
occ:0.61
CU A:CU501 0.0 30.3 0.6
NE2 A:HIS38 1.9 16.5 0.6
CU A:CU501 2.0 17.5 0.4
O B:HOH784 2.0 34.2 1.0
NE2 A:HIS54 2.1 21.5 1.0
O B:HOH555 2.1 16.9 1.0
NE2 A:HIS38 2.5 15.0 0.4
NE2 A:HIS63 2.5 10.8 1.0
CE1 A:HIS38 2.6 16.1 0.6
CD2 A:HIS38 2.9 13.2 0.4
CD2 A:HIS54 3.0 20.6 1.0
CE1 A:HIS54 3.0 17.4 1.0
CE1 A:HIS63 3.1 8.5 1.0
CD2 A:HIS38 3.1 14.7 0.6
OE2 B:DAH98 3.5 10.2 0.3
CD2 A:HIS63 3.7 10.0 1.0
CE1 A:HIS38 3.7 13.0 0.4
CU A:CU502 3.8 20.0 1.0
ND1 A:HIS38 3.8 13.0 0.6
OZ B:DAH98 3.8 17.9 1.0
CG A:HIS38 4.0 12.5 0.6
CG A:HIS54 4.1 16.8 1.0
ND1 A:HIS54 4.1 15.8 1.0
NE2 A:HIS216 4.2 10.4 1.0
CG A:HIS38 4.2 12.0 0.4
CE2 A:PHE212 4.3 11.5 1.0
CZ A:PHE212 4.3 9.2 1.0
ND1 A:HIS63 4.3 8.5 1.0
CE1 A:HIS216 4.5 9.8 1.0
CG1 A:ILE42 4.5 35.4 1.0
ND1 A:HIS38 4.6 11.8 0.4
CG A:HIS63 4.7 8.6 1.0
CE2 B:DAH98 4.7 13.6 1.0
CZ B:DAH98 4.8 14.9 1.0
NE2 A:HIS190 4.9 9.7 1.0
CE1 A:PHE59 5.0 9.4 1.0

Copper binding site 2 out of 6 in 2zwe

Go back to Copper Binding Sites List in 2zwe
Copper binding site 2 out of 6 in the Crystal Structure of the Copper-Bound Tyrosinase in Complex with A Caddie Protein From Streptomyces Castaneoglobisporus Obtained By Soaking the Deoxy-Form Crystal in Dioxygen-Saturated Solution For 40 Minutes


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure of the Copper-Bound Tyrosinase in Complex with A Caddie Protein From Streptomyces Castaneoglobisporus Obtained By Soaking the Deoxy-Form Crystal in Dioxygen-Saturated Solution For 40 Minutes within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu501

b:17.5
occ:0.39
CU A:CU501 0.0 17.5 0.4
CU A:CU501 2.0 30.3 0.6
OZ B:DAH98 2.0 17.9 1.0
OE2 B:DAH98 2.1 10.2 0.3
NE2 A:HIS38 2.2 15.0 0.4
O B:HOH555 2.2 16.9 1.0
CE1 A:HIS38 2.3 16.1 0.6
NE2 A:HIS54 2.5 21.5 1.0
O B:HOH784 2.6 34.2 1.0
NE2 A:HIS38 2.7 16.5 0.6
CE1 A:HIS38 2.8 13.0 0.4
CZ B:DAH98 3.0 14.9 1.0
CE2 B:DAH98 3.1 13.6 1.0
CD2 A:HIS54 3.2 20.6 1.0
CG1 A:ILE42 3.4 35.4 1.0
CD2 A:HIS38 3.4 13.2 0.4
CD1 A:ILE42 3.4 35.3 1.0
ND1 A:HIS38 3.6 13.0 0.6
CE1 A:HIS54 3.6 17.4 1.0
CU A:CU502 3.9 20.0 1.0
CD2 A:HIS38 4.1 14.7 0.6
ND1 A:HIS38 4.1 11.8 0.4
OG A:SER206 4.2 10.0 1.0
CE1 B:DAH98 4.3 11.7 1.0
CG A:HIS54 4.4 16.8 1.0
CG A:HIS38 4.4 12.0 0.4
CD2 B:DAH98 4.4 12.3 1.0
CG A:HIS38 4.5 12.5 0.6
NE2 A:HIS63 4.5 10.8 1.0
NE2 A:HIS194 4.5 8.1 1.0
ND1 A:HIS54 4.5 15.8 1.0
CE1 A:HIS194 4.6 8.3 1.0
O B:HOH627 4.6 21.9 1.0
CE2 A:PHE212 4.6 11.5 1.0
CB A:ILE42 4.8 22.2 1.0
CE1 A:HIS63 4.9 8.5 1.0

Copper binding site 3 out of 6 in 2zwe

Go back to Copper Binding Sites List in 2zwe
Copper binding site 3 out of 6 in the Crystal Structure of the Copper-Bound Tyrosinase in Complex with A Caddie Protein From Streptomyces Castaneoglobisporus Obtained By Soaking the Deoxy-Form Crystal in Dioxygen-Saturated Solution For 40 Minutes


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Crystal Structure of the Copper-Bound Tyrosinase in Complex with A Caddie Protein From Streptomyces Castaneoglobisporus Obtained By Soaking the Deoxy-Form Crystal in Dioxygen-Saturated Solution For 40 Minutes within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu502

b:20.0
occ:1.00
O B:HOH555 1.9 16.9 1.0
NE2 A:HIS194 2.0 8.1 1.0
NE2 A:HIS190 2.0 9.7 1.0
NE2 A:HIS216 2.0 10.4 1.0
O B:HOH784 2.7 34.2 1.0
CE1 A:HIS216 2.9 9.8 1.0
CE1 A:HIS194 2.9 8.3 1.0
CE1 A:HIS190 3.0 9.7 1.0
CD2 A:HIS190 3.0 10.1 1.0
CD2 A:HIS194 3.0 8.9 1.0
CD2 A:HIS216 3.1 10.8 1.0
OE2 B:DAH98 3.4 10.2 0.3
CU A:CU501 3.8 30.3 0.6
CU A:CU501 3.9 17.5 0.4
CE2 B:DAH98 4.0 13.6 1.0
ND1 A:HIS194 4.1 9.3 1.0
ND1 A:HIS216 4.1 9.7 1.0
OZ B:DAH98 4.1 17.9 1.0
ND1 A:HIS190 4.1 9.2 1.0
CE2 A:PHE212 4.1 11.5 1.0
CG A:HIS194 4.1 8.7 1.0
CG A:HIS190 4.1 9.6 1.0
CG A:HIS216 4.2 8.8 1.0
CD2 A:HIS215 4.3 11.5 1.0
CZ B:DAH98 4.4 14.9 1.0
NE2 A:HIS215 4.5 10.7 1.0
NE2 A:HIS63 4.6 10.8 1.0
CD2 A:PHE212 4.7 8.5 1.0
CZ A:PHE212 4.8 9.2 1.0
CD2 B:DAH98 4.8 12.3 1.0
CE1 A:PHE59 4.9 9.4 1.0
NE2 A:HIS38 5.0 16.5 0.6

Copper binding site 4 out of 6 in 2zwe

Go back to Copper Binding Sites List in 2zwe
Copper binding site 4 out of 6 in the Crystal Structure of the Copper-Bound Tyrosinase in Complex with A Caddie Protein From Streptomyces Castaneoglobisporus Obtained By Soaking the Deoxy-Form Crystal in Dioxygen-Saturated Solution For 40 Minutes


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Crystal Structure of the Copper-Bound Tyrosinase in Complex with A Caddie Protein From Streptomyces Castaneoglobisporus Obtained By Soaking the Deoxy-Form Crystal in Dioxygen-Saturated Solution For 40 Minutes within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu504

b:40.3
occ:0.50
NE2 A:HIS277 2.0 30.1 1.0
O A:HOH939 2.4 29.3 0.5
CE1 A:HIS277 2.8 31.3 1.0
CD2 A:HIS277 3.1 32.6 1.0
ND1 A:HIS277 4.0 32.3 1.0
CG A:HIS277 4.2 36.7 1.0
O A:HOH934 4.4 54.6 1.0
CG A:PRO231 4.6 22.7 1.0
O A:HOH814 4.7 34.9 1.0

Copper binding site 5 out of 6 in 2zwe

Go back to Copper Binding Sites List in 2zwe
Copper binding site 5 out of 6 in the Crystal Structure of the Copper-Bound Tyrosinase in Complex with A Caddie Protein From Streptomyces Castaneoglobisporus Obtained By Soaking the Deoxy-Form Crystal in Dioxygen-Saturated Solution For 40 Minutes


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 5 of Crystal Structure of the Copper-Bound Tyrosinase in Complex with A Caddie Protein From Streptomyces Castaneoglobisporus Obtained By Soaking the Deoxy-Form Crystal in Dioxygen-Saturated Solution For 40 Minutes within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu503

b:18.2
occ:0.57
NE2 B:HIS82 2.0 12.8 0.6
NE2 B:HIS97 2.1 26.8 1.0
SD B:MET84 2.2 26.1 1.0
CG B:MET84 2.9 24.9 1.0
CE1 B:HIS82 3.0 15.5 0.6
CE1 B:HIS97 3.0 22.4 1.0
CD2 B:HIS82 3.1 14.5 0.6
CD2 B:HIS97 3.2 24.8 1.0
O A:ILE42 3.3 16.7 1.0
CE B:MET84 3.3 23.6 1.0
CE1 B:HIS82 3.4 15.5 0.4
ND1 B:HIS82 3.5 14.7 0.4
CB B:MET84 3.6 14.7 1.0
CA A:MET43 3.7 16.3 1.0
O A:MET43 4.0 19.1 1.0
ND1 B:HIS82 4.1 15.3 0.6
C A:MET43 4.1 15.4 1.0
ND1 B:HIS97 4.2 19.5 1.0
C A:ILE42 4.2 17.8 1.0
CG B:HIS82 4.2 12.1 0.6
CG B:HIS97 4.3 15.7 1.0
N A:MET43 4.4 13.5 1.0
O A:HOH634 4.6 22.3 1.0
CB A:MET43 4.6 17.1 1.0
NE2 B:HIS82 4.7 16.7 0.4
CA B:MET84 4.8 13.8 1.0
CG A:MET43 4.8 19.3 1.0
N B:MET84 4.8 12.7 1.0
CG B:HIS82 4.8 13.7 0.4
C B:VAL83 5.0 12.1 1.0

Copper binding site 6 out of 6 in 2zwe

Go back to Copper Binding Sites List in 2zwe
Copper binding site 6 out of 6 in the Crystal Structure of the Copper-Bound Tyrosinase in Complex with A Caddie Protein From Streptomyces Castaneoglobisporus Obtained By Soaking the Deoxy-Form Crystal in Dioxygen-Saturated Solution For 40 Minutes


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 6 of Crystal Structure of the Copper-Bound Tyrosinase in Complex with A Caddie Protein From Streptomyces Castaneoglobisporus Obtained By Soaking the Deoxy-Form Crystal in Dioxygen-Saturated Solution For 40 Minutes within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu503

b:21.2
occ:0.43
OE1 B:GLU67 1.4 68.2 1.0
ND1 B:HIS68 1.8 54.2 1.0
NE2 B:HIS82 2.0 16.7 0.4
O B:HIS68 2.2 55.4 1.0
N B:HIS68 2.6 61.8 1.0
CD B:GLU67 2.7 67.3 1.0
CE1 B:HIS68 2.7 55.4 1.0
C B:HIS68 2.8 58.4 1.0
CG B:HIS68 2.9 56.2 1.0
CD2 B:HIS82 3.0 16.0 0.4
CA B:HIS68 3.0 59.7 1.0
CE1 B:HIS82 3.0 15.5 0.4
C B:GLU67 3.3 64.5 1.0
CB B:HIS68 3.4 57.8 1.0
OE2 B:GLU67 3.4 62.9 1.0
ND1 B:HIS82 3.5 15.3 0.6
CB B:GLU67 3.5 67.3 1.0
CE1 B:HIS82 3.6 15.5 0.6
CG B:GLU67 3.7 67.2 1.0
O B:GLY69 3.8 69.9 1.0
NE2 B:HIS68 3.9 56.0 1.0
N B:GLY69 3.9 60.8 1.0
CD2 B:HIS68 4.0 55.9 1.0
CA B:GLU67 4.0 66.3 1.0
O B:GLU67 4.0 67.7 1.0
O A:MET43 4.1 19.1 1.0
ND1 B:HIS82 4.1 14.7 0.4
CG B:HIS82 4.1 13.7 0.4
C B:GLY69 4.2 61.6 1.0
CA B:GLY69 4.6 62.4 1.0
O B:HOH562 4.7 17.2 1.0
CG B:HIS82 4.8 12.1 0.6
NE2 B:HIS82 4.9 12.8 0.6
N B:GLY70 4.9 57.2 1.0

Reference:

Y.Matoba, H.Yoshitsu, H.J.Jeon, K.Oda, M.Noda, T.Kumagai, M.Sugiyama. Crystallographic Evidence of Drastic Movement of A Copper Ion Toward the Substrate Tyrosine For Starting Hydroxylation Reaction of Tyrosinase To Be Published.
Page generated: Sun Dec 13 11:08:32 2020

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