Copper in the structure of The 1.37-A Crystal Structure Of The Hydroxylamine-Induced Deoxy-Form of the Copper-Bound Tyrosinase in Complex With A Caddie Protein From Streptomyces Castaneoglobisporus (pdb 2zmz)

The binding sites of Copper atom in the structure of The 1.37-A Crystal Structure Of The Hydroxylamine-Induced Deoxy-Form of the Copper-Bound Tyrosinase in Complex With A Caddie Protein From Streptomyces Castaneoglobisporus (pdb code 2zmz). This binding sites where shown with 5.0 Angstroms radius around Copper atom. The 2zmz structure was solved by Y.MATOBA, M.SUGIYAMA, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 30.0-1.4 Space group P21212 a (A) 65.180 b (A) 98.030 c (A) 55.200 alpha (°) 90.00 beta (°) 90.00 gamma (°) 90.00 Rfactor (%) 18.6 Rfree (%) 23.2 Copper Binding Sites: Copper binding site 1 out of 4 in 2zmz Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Copper in the PDB 2zmz. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: His38, A: Gly53, A: His54, A: Trp62, A: His63, A: Phe212, A: His216, B: Tyr98, A: Cu1302, B: Hoh340, conact list: Atom Atom Distance (A) Cu NE2 A:His38 1.86 Cu ND1 A:His38 3.93 Cu CD2 A:His38 2.77 Cu CE1 A:His38 2.88 Cu CG A:His38 3.92 Cu O A:Gly53 5.00 Cu NE2 A:His54 2.12 Cu ND1 A:His54 4.26 Cu CD2 A:His54 3.00 Cu CE1 A:His54 3.19 Cu CG A:His54 4.18 Cu CZ3 A:Trp62 4.71 Cu NE2 A:His63 2.16 Cu ND1 A:His63 4.14 Cu CD2 A:His63 3.35 Cu CE1 A:His63 2.92 Cu CG A:His63 4.38 Cu CE2 A:Phe212 4.30 Cu CZ A:Phe212 4.10 Cu NE2 A:His216 4.25 Cu CE1 A:His216 4.54 Cu OH B:Tyr98 4.35 Cu CU A:Cu1302 4.07 Cu O B:Hoh340 2.35 interactive model: Copper binding site 2 out of 4 in 2zmz Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Copper in the PDB 2zmz. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Phe59, A: His63, A: His190, A: His194, A: Phe212, A: His215, A: His216, B: Tyr98, A: Cu1301, B: Hoh340, conact list: Atom Atom Distance (A) Cu CE1 A:Phe59 4.90 Cu NE2 A:His63 4.66 Cu NE2 A:His190 2.01 Cu ND1 A:His190 4.10 Cu CD2 A:His190 2.99 Cu CE1 A:His190 3.00 Cu CG A:His190 4.13 Cu NE2 A:His194 1.98 Cu ND1 A:His194 4.10 Cu CD2 A:His194 3.05 Cu CE1 A:His194 2.96 Cu CG A:His194 4.18 Cu CE2 A:Phe212 4.06 Cu CD2 A:Phe212 4.75 Cu CZ A:Phe212 4.67 Cu NE2 A:His215 4.52 Cu CD2 A:His215 4.31 Cu NE2 A:His216 2.01 Cu ND1 A:His216 4.06 Cu CD2 A:His216 3.10 Cu CE1 A:His216 2.90 Cu CG A:His216 4.18 Cu CE2 B:Tyr98 4.13 Cu CZ B:Tyr98 4.36 Cu OH B:Tyr98 4.08 Cu CU A:Cu1301 4.07 Cu O B:Hoh340 2.03 interactive model: Copper binding site 3 out of 4 in 2zmz Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 3 of Copper in the PDB 2zmz. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Ile42, A: Met43, B: His82, B: Val83, B: Met84, B: Ile92, B: His97, B: No3304, A: Hoh412, conact list: Atom Atom Distance (A) Cu O A:Ile42 3.30 Cu C A:Ile42 4.28 Cu O A:Met43 4.01 Cu N A:Met43 4.54 Cu CB A:Met43 4.83 Cu C A:Met43 4.25 Cu CG A:Met43 4.87 Cu CA A:Met43 3.85 Cu NE2 B:His82 2.04 Cu NE2 B:His82 4.87 Cu ND1 B:His82 4.10 Cu ND1 B:His82 3.56 Cu CD2 B:His82 3.10 Cu CE1 B:His82 2.98 Cu CE1 B:His82 3.56 Cu CG B:His82 4.23 Cu CG B:His82 4.92 Cu C B:Val83 4.95 Cu N B:Met84 4.75 Cu CB B:Met84 3.46 Cu CE B:Met84 3.26 Cu CG B:Met84 3.26 Cu SD B:Met84 2.36 Cu CA B:Met84 4.68 Cu CD1 B:Ile92 4.78 Cu CG1 B:Ile92 4.84 Cu NE2 B:His97 2.30 Cu NE2 B:His97 2.97 Cu ND1 B:His97 4.15 Cu ND1 B:His97 3.94 Cu CD2 B:His97 3.52 Cu CD2 B:His97 4.20 Cu CE1 B:His97 2.90 Cu CE1 B:His97 2.78 Cu CG B:His97 4.47 Cu CG B:His97 4.69 Cu O1 B:No3304 3.03 Cu O2 B:No3304 2.98 Cu N B:No3304 2.58 Cu O3 B:No3304 2.52 Cu O A:Hoh412 4.55 interactive model: Copper binding site 4 out of 4 in 2zmz Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 4 of Copper in the PDB 2zmz. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Met43, B: Glu67, B: His68, B: Gly69, B: His82, B: Hoh424, conact list: Atom Atom Distance (A) Cu O A:Met43 4.16 Cu O B:Glu67 4.80 Cu N B:Glu67 4.49 Cu OE1 B:Glu67 2.30 Cu CB B:Glu67 2.16 Cu OE2 B:Glu67 4.13 Cu CD B:Glu67 2.89 Cu C B:Glu67 3.59 Cu CG B:Glu67 2.80 Cu CA B:Glu67 3.44 Cu O B:His68 4.14 Cu NE2 B:His68 3.41 Cu N B:His68 2.79 Cu CB B:His68 3.85 Cu ND1 B:His68 1.69 Cu CD2 B:His68 3.82 Cu C B:His68 4.07 Cu CE1 B:His68 2.15 Cu CG B:His68 3.01 Cu CA B:His68 3.70 Cu O B:Gly69 4.84 Cu N B:Gly69 4.86 Cu C B:Gly69 4.99 Cu NE2 B:His82 4.92 Cu NE2 B:His82 1.96 Cu ND1 B:His82 3.44 Cu ND1 B:His82 4.08 Cu CD2 B:His82 2.88 Cu CE1 B:His82 3.59 Cu CE1 B:His82 3.02 Cu CG B:His82 4.76 Cu CG B:His82 4.06 Cu O B:Hoh424 4.71 interactive model: