Copper in the structure of Pseudomonas Aeruginosa Azurin With Mutated Metal-Binding Loop Sequence (Caahaam), Chemically Reduced, PH4.2 (pdb 2xv2)

The binding sites of Copper atom in the structure of Pseudomonas Aeruginosa Azurin With Mutated Metal-Binding Loop Sequence (Caahaam), Chemically Reduced, PH4.2 (pdb code 2xv2). This binding sites where shown with 5.0 Angstroms radius around Copper atom. The 2xv2 structure was solved by C.LI, K.SATO, S.MONARI, I.SALARD, M.SOLA, M.J.BANFIELD, C.DENNISON, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 36.1-1.6 Space group P212121 a (A) 39.897 b (A) 46.249 c (A) 57.834 alpha (°) 90.00 beta (°) 90.00 gamma (°) 90.00 Rfactor (%) 14.017 Rfree (%) 20.039 Copper Binding Sites: Copper binding site 1 out of 1 in 2xv2 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Copper in the PDB 2xv2. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Gly45, A: His46, A: Asn47, A: Cys112, A: Ala114, A: His115, A: Met118, conact list: Atom Atom Distance (A) Cu O A:Gly45 3.61 Cu C A:Gly45 4.37 Cu NE2 A:His46 4.10 Cu N A:His46 4.37 Cu CB A:His46 3.40 Cu ND1 A:His46 2.02 Cu CD2 A:His46 4.16 Cu C A:His46 4.54 Cu CE1 A:His46 2.98 Cu CG A:His46 3.05 Cu CA A:His46 3.48 Cu O A:Asn47 4.88 Cu N A:Asn47 4.47 Cu CB A:Cys112 3.04 Cu SG A:Cys112 2.13 Cu CA A:Cys112 4.47 Cu C A:Ala114 4.96 Cu CB A:Ala114 4.64 Cu O A:His115 4.63 Cu NE2 A:His115 4.04 Cu N A:His115 4.21 Cu CB A:His115 3.59 Cu ND1 A:His115 4.82 Cu CD2 A:His115 2.84 Cu C A:His115 4.98 Cu CG A:His115 3.51 Cu CA A:His115 4.46 Cu CB A:Met118 4.74 Cu CE A:Met118 3.39 Cu CG A:Met118 4.32 Cu SD A:Met118 2.81 interactive model: