Copper in PDB 2xla: Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Cu- Cuca-Closed

The structure of Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Cu- Cuca-Closed, PDB code: 2xla was solved by K.J.Waldron, S.J.Firbank, S.J.Dainty, M.Perez-Rama, S.Tottey, N.J.Robinson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	37.42 / 1.93
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	50.310, 72.510, 262.100, 90.00, 90.00, 90.00
R / Rfree (%)	16.1 / 20.3

The binding sites of Copper atom in the Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Cu- Cuca-Closed (pdb code 2xla). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 4 binding sites of Copper where determined in the Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Cu- Cuca-Closed, PDB code: 2xla:
Jump to Copper binding site number: 1; 2; 3; 4;

Copper binding site 1 out of 4 in the Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Cu- Cuca-Closed


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Cu- Cuca-Closed within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu1298 b:4.9 occ:1.00 NE2 A:HIS119 2.1 5.1 1.0 NE2 A:HIS117 2.1 4.2 1.0 OE1 A:GLU124 2.1 12.0 1.0 NE2 A:HIS178 2.2 9.7 1.0 O A:HOH2318 2.2 25.3 1.0 O A:HOH2080 2.7 25.8 1.0 CD2 A:HIS119 2.9 4.2 1.0 CD2 A:HIS117 3.0 5.6 1.0 CD A:GLU124 3.1 8.5 1.0 CD2 A:HIS178 3.1 7.9 1.0 CE1 A:HIS119 3.1 8.2 1.0 CE1 A:HIS117 3.2 8.4 1.0 CE1 A:HIS178 3.2 10.1 1.0 OE2 A:GLU124 3.4 9.0 1.0 O A:HOH2319 3.9 18.4 1.0 CG A:HIS119 4.1 5.3 1.0 CG A:HIS117 4.2 5.9 1.0 ND1 A:HIS119 4.2 6.4 1.0 N2 A:URE1299 4.2 13.6 1.0 ND1 A:HIS117 4.2 7.0 1.0 ND1 A:HIS178 4.3 9.9 1.0 CG A:HIS178 4.3 8.7 1.0 CG A:GLU124 4.5 6.0 1.0 CZ A:PHE126 4.5 8.7 1.0 NE1 A:TRP194 4.7 9.6 1.0 CB A:GLU124 4.8 4.0 1.0 CE1 A:PHE126 5.0 7.8 1.0

Copper binding site 2 out of 4 in the Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Cu- Cuca-Closed


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Cu- Cuca-Closed within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu1298 b:6.0 occ:1.00 NE2 B:HIS119 2.1 9.4 1.0 NE2 B:HIS117 2.1 6.4 1.0 NE2 B:HIS178 2.2 12.8 1.0 OE1 B:GLU124 2.2 13.5 1.0 O B:HOH2247 2.3 20.1 1.0 CD2 B:HIS119 3.0 8.6 1.0 CD2 B:HIS117 3.0 7.7 1.0 CE1 B:HIS117 3.1 8.3 1.0 CE1 B:HIS178 3.1 13.3 1.0 CE1 B:HIS119 3.2 8.1 1.0 CD B:GLU124 3.2 10.7 1.0 CD2 B:HIS178 3.2 11.7 1.0 O B:HOH2058 3.2 35.0 1.0 OE2 B:GLU124 3.5 14.2 1.0 O B:HOH2246 3.8 19.0 1.0 CG B:HIS119 4.2 8.3 1.0 CG B:HIS117 4.2 6.0 1.0 ND1 B:HIS117 4.2 6.5 1.0 ND1 B:HIS119 4.2 9.6 1.0 ND1 B:HIS178 4.3 12.1 1.0 CG B:HIS178 4.3 8.8 1.0 NE1 B:TRP194 4.4 8.8 1.0 CZ B:PHE126 4.4 7.4 1.0 N2 B:URE1299 4.5 15.2 1.0 CG B:GLU124 4.5 7.5 1.0 CB B:GLU124 4.8 6.7 1.0 CD1 B:TRP194 4.8 7.9 1.0 CE1 B:PHE126 4.9 8.7 1.0

Copper binding site 3 out of 4 in the Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Cu- Cuca-Closed


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Cu- Cuca-Closed within 5.0Å range: probe atom residue distance (Å) B Occ C:Cu1298 b:7.6 occ:1.00 NE2 C:HIS119 2.0 8.9 1.0 NE2 C:HIS117 2.1 11.4 1.0 NE2 C:HIS178 2.1 12.7 1.0 OE1 C:GLU124 2.2 12.9 1.0 O C:HOH2279 2.4 21.2 1.0 O C:HOH2071 2.5 26.1 1.0 CD2 C:HIS119 2.9 5.4 1.0 CD2 C:HIS117 3.0 10.7 1.0 CE1 C:HIS178 3.0 13.2 1.0 CD2 C:HIS178 3.1 11.4 1.0 CE1 C:HIS117 3.1 11.8 1.0 CE1 C:HIS119 3.1 7.8 1.0 CD C:GLU124 3.2 11.9 1.0 OE2 C:GLU124 3.5 16.3 1.0 O C:HOH2278 3.9 18.8 1.0 CG C:HIS119 4.1 7.3 1.0 CG C:HIS117 4.1 10.0 1.0 ND1 C:HIS117 4.2 10.8 1.0 ND1 C:HIS178 4.2 13.5 1.0 ND1 C:HIS119 4.2 7.6 1.0 CG C:HIS178 4.2 10.9 1.0 N1 C:URE1299 4.3 18.2 1.0 NE1 C:TRP194 4.5 8.4 1.0 CG C:GLU124 4.5 9.6 1.0 CZ C:PHE126 4.5 13.6 1.0 CB C:GLU124 4.8 8.6 1.0 CD1 C:TRP194 4.9 8.8 1.0 CE1 C:PHE126 5.0 13.0 1.0

Copper binding site 4 out of 4 in the Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Cu- Cuca-Closed


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Structure and Metal-Loading of A Soluble Periplasm Cupro-Protein: Cu- Cuca-Closed within 5.0Å range: probe atom residue distance (Å) B Occ D:Cu1298 b:7.8 occ:1.00 O D:HOH2047 1.9 33.8 1.0 NE2 D:HIS117 2.1 10.8 1.0 NE2 D:HIS119 2.1 8.2 1.0 OE1 D:GLU124 2.2 12.6 1.0 NE2 D:HIS178 2.2 13.7 1.0 CD2 D:HIS119 2.9 9.8 1.0 CD2 D:HIS117 3.0 11.0 1.0 CE1 D:HIS117 3.1 10.5 1.0 CD2 D:HIS178 3.1 9.9 1.0 CE1 D:HIS119 3.2 9.9 1.0 CD D:GLU124 3.2 15.1 1.0 CE1 D:HIS178 3.2 13.6 1.0 OE2 D:GLU124 3.5 18.9 1.0 O D:HOH2228 3.9 22.5 1.0 CG D:HIS119 4.1 10.2 1.0 CG D:HIS117 4.1 8.7 1.0 ND1 D:HIS117 4.1 9.6 1.0 ND1 D:HIS119 4.2 9.3 1.0 CG D:HIS178 4.3 10.7 1.0 ND1 D:HIS178 4.3 14.4 1.0 N1 D:URE1299 4.4 17.8 1.0 NE1 D:TRP194 4.6 11.2 1.0 CG D:GLU124 4.6 12.8 1.0 CZ D:PHE126 4.6 14.0 1.0 CB D:GLU124 4.8 12.2 1.0

K.J.Waldron, S.J.Firbank, S.J.Dainty, M.Perez-Rama, S.Tottey, N.J.Robinson. Structure and Metal Loading of A Soluble Periplasm Cuproprotein. J.Biol.Chem. V. 285 32504 2010.
ISSN: ISSN 0021-9258
PubMed: 20702411
DOI: 10.1074/JBC.M110.153080