Copper in the structure of Edta Treated E. Coli Copper Amine Oxidase (pdb 2wof)

The binding sites of Copper atom in the structure of Edta Treated E. Coli Copper Amine Oxidase (pdb code 2wof). This binding sites where shown with 5.0 Angstroms radius around Copper atom. The 2wof structure was solved by M.A.SMITH, P.PIRRAT, A.R.PEARSON, P.F.KNOWLES, S.E.V.PHILLIPS, M.J.MCPHERSON, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 20.0-2.2 Space group P212121 a (A) 135.079 b (A) 166.880 c (A) 79.802 alpha (°) 90.00 beta (°) 90.00 gamma (°) 90.00 Rfactor (%) 16.686 Rfree (%) 22.041 Copper Binding Sites: Copper binding site 1 out of 2 in 2wof Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Copper in the PDB 2wof. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Tpq466, A: His524, A: His526, A: His689, A: Met699, A: Hoh2341, A: Hoh2434, conact list: Atom Atom Distance (A) Cu O5 A:Tpq466 3.44 Cu C6 A:Tpq466 4.99 Cu C5 A:Tpq466 3.71 Cu O2 A:Tpq466 4.69 Cu C4 A:Tpq466 3.22 Cu C3 A:Tpq466 4.30 Cu O4 A:Tpq466 2.26 Cu NE2 A:His524 2.17 Cu ND1 A:His524 4.23 Cu CD2 A:His524 3.07 Cu CE1 A:His524 3.16 Cu CG A:His524 4.23 Cu NE2 A:His526 2.85 Cu ND1 A:His526 2.94 Cu CD2 A:His526 4.03 Cu CE1 A:His526 1.92 Cu CG A:His526 4.08 Cu NE2 A:His689 4.34 Cu CB A:His689 3.48 Cu ND1 A:His689 2.24 Cu CD2 A:His689 4.36 Cu CE1 A:His689 3.22 Cu CG A:His689 3.22 Cu CE A:Met699 4.75 Cu CE A:Met699 4.46 Cu SD A:Met699 4.97 Cu SD A:Met699 4.80 Cu O A:Hoh2341 4.74 Cu O A:Hoh2434 2.85 interactive model: Copper binding site 2 out of 2 in 2wof Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Copper in the PDB 2wof. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: B: Tpq466, B: His524, B: His526, B: His689, B: Met699, B: Hoh2231, B: Hoh2307, conact list: Atom Atom Distance (A) Cu O5 B:Tpq466 3.87 Cu C5 B:Tpq466 3.86 Cu O2 B:Tpq466 4.80 Cu C4 B:Tpq466 3.03 Cu C3 B:Tpq466 3.91 Cu O4 B:Tpq466 1.91 Cu NE2 B:His524 2.11 Cu ND1 B:His524 4.10 Cu CD2 B:His524 3.17 Cu CE1 B:His524 2.96 Cu CG B:His524 4.23 Cu NE2 B:His526 2.08 Cu ND1 B:His526 4.15 Cu CD2 B:His526 2.98 Cu CE1 B:His526 3.09 Cu CG B:His526 4.13 Cu NE2 B:His689 4.32 Cu CB B:His689 3.49 Cu ND1 B:His689 2.23 Cu CD2 B:His689 4.34 Cu CE1 B:His689 3.20 Cu CG B:His689 3.20 Cu CE B:Met699 4.84 Cu CE B:Met699 4.54 Cu SD B:Met699 4.89 Cu SD B:Met699 4.52 Cu O B:Hoh2231 4.79 Cu O B:Hoh2307 3.05 interactive model: