Copper in PDB 2pnc: Crystal Structure of Bovine Plasma Copper-Containing Amine Oxidase in Complex with Clonidine

All present enzymatic activity of Crystal Structure of Bovine Plasma Copper-Containing Amine Oxidase in Complex with Clonidine:
1.4.3.6;

The structure of Crystal Structure of Bovine Plasma Copper-Containing Amine Oxidase in Complex with Clonidine, PDB code: 2pnc was solved by L.Cendron, A.Holt, D.J.Smith, G.Zanotti, A.Rigo, M.L.Di Paolo, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.11 / 2.40
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	78.360, 131.956, 134.159, 90.00, 90.00, 90.00
R / Rfree (%)	23.7 / 25.2

The structure of Crystal Structure of Bovine Plasma Copper-Containing Amine Oxidase in Complex with Clonidine also contains other interesting chemical elements:

Chlorine	(Cl)	7 atoms
Calcium	(Ca)	4 atoms

The binding sites of Copper atom in the Crystal Structure of Bovine Plasma Copper-Containing Amine Oxidase in Complex with Clonidine (pdb code 2pnc). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Crystal Structure of Bovine Plasma Copper-Containing Amine Oxidase in Complex with Clonidine, PDB code: 2pnc:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the Crystal Structure of Bovine Plasma Copper-Containing Amine Oxidase in Complex with Clonidine


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure of Bovine Plasma Copper-Containing Amine Oxidase in Complex with Clonidine within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu804 b:29.2 occ:1.00 O4 A:TPQ470 2.1 35.7 1.0 ND1 A:HIS683 2.1 19.8 1.0 NE2 A:HIS519 2.1 16.1 1.0 NE2 A:HIS521 2.1 36.6 1.0 CE1 A:HIS683 2.8 19.8 1.0 CE1 A:HIS521 3.0 34.0 1.0 CD2 A:HIS519 3.1 10.5 1.0 CE1 A:HIS519 3.2 5.1 1.0 CD2 A:HIS521 3.2 34.9 1.0 CG A:HIS683 3.3 13.9 1.0 C4 A:TPQ470 3.3 35.5 1.0 O5 A:TPQ470 3.8 34.3 1.0 CB A:HIS683 3.9 18.0 1.0 C5 A:TPQ470 4.0 37.2 1.0 NE2 A:HIS683 4.0 9.6 1.0 ND1 A:HIS521 4.2 34.5 1.0 CG A:HIS519 4.2 12.2 1.0 ND1 A:HIS519 4.3 6.2 1.0 CD2 A:HIS683 4.3 11.4 1.0 CG A:HIS521 4.3 31.5 1.0 C3 A:TPQ470 4.4 36.6 1.0 O A:HOH901 4.5 21.8 1.0 CG2 A:ILE494 4.5 23.7 1.0

Copper binding site 2 out of 2 in the Crystal Structure of Bovine Plasma Copper-Containing Amine Oxidase in Complex with Clonidine


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure of Bovine Plasma Copper-Containing Amine Oxidase in Complex with Clonidine within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu804 b:27.9 occ:1.00 NE2 B:HIS519 2.1 15.2 1.0 NE2 B:HIS521 2.1 33.5 1.0 O4 B:TPQ470 2.1 42.0 1.0 ND1 B:HIS683 2.2 18.6 1.0 CE1 B:HIS521 3.0 32.6 1.0 CE1 B:HIS683 3.0 20.2 1.0 CE1 B:HIS519 3.1 17.0 1.0 CD2 B:HIS519 3.1 19.9 1.0 CD2 B:HIS521 3.2 30.0 1.0 C4 B:TPQ470 3.2 40.5 1.0 CG B:HIS683 3.3 18.3 1.0 O B:HOH1021 3.6 50.0 1.0 CB B:HIS683 3.7 17.1 1.0 C3 B:TPQ470 4.0 42.3 1.0 C5 B:TPQ470 4.1 40.8 1.0 ND1 B:HIS521 4.2 34.4 1.0 ND1 B:HIS519 4.2 14.9 1.0 O5 B:TPQ470 4.2 37.5 1.0 NE2 B:HIS683 4.2 16.9 1.0 CG B:HIS519 4.2 18.4 1.0 CG B:HIS521 4.3 31.1 1.0 CD2 B:HIS683 4.4 14.8 1.0 O B:HOH902 4.4 26.4 1.0 CG2 B:ILE494 4.8 25.3 1.0

A.Holt, D.J.Smith, L.Cendron, G.Zanotti, A.Rigo, M.L.Di Paolo. Multiple Binding Sites For Substrates and Modulators of Semicarbazide-Sensitive Amine Oxidases: Kinetic Consequences Mol.Pharmacol. V. 73 525 2008.
ISSN: ISSN 0026-895X
PubMed: 17989349
DOI: 10.1124/MOL.107.040964