Copper in PDB 2hx9: Crystal Structure of Cu(I) Azurin with the Metal-Binding Loop Sequence "Ctfpghsalm" Replaced with "Csphqgagm", at PH4

The structure of Crystal Structure of Cu(I) Azurin with the Metal-Binding Loop Sequence "Ctfpghsalm" Replaced with "Csphqgagm", at PH4, PDB code: 2hx9 was solved by M.J.Banfield, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.00 / 1.70
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	38.420, 65.335, 97.242, 90.00, 90.00, 90.00
R / Rfree (%)	17 / 21.8

The binding sites of Copper atom in the Crystal Structure of Cu(I) Azurin with the Metal-Binding Loop Sequence "Ctfpghsalm" Replaced with "Csphqgagm", at PH4 (pdb code 2hx9). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Crystal Structure of Cu(I) Azurin with the Metal-Binding Loop Sequence "Ctfpghsalm" Replaced with "Csphqgagm", at PH4, PDB code: 2hx9:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the Crystal Structure of Cu(I) Azurin with the Metal-Binding Loop Sequence "Ctfpghsalm" Replaced with "Csphqgagm", at PH4


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure of Cu(I) Azurin with the Metal-Binding Loop Sequence "Ctfpghsalm" Replaced with "Csphqgagm", at PH4 within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu201 b:17.8 occ:1.00 ND1 A:HIS46 2.1 12.9 1.0 ND1 A:HIS115 2.2 15.4 1.0 SG A:CYS112 2.2 12.0 1.0 CE1 A:HIS46 3.0 12.2 1.0 CG A:HIS46 3.1 11.9 1.0 CG A:HIS115 3.1 13.7 1.0 SD A:MET120 3.1 15.6 1.0 CE1 A:HIS115 3.2 15.7 1.0 O A:GLY45 3.2 13.3 1.0 CB A:CYS112 3.2 12.0 1.0 CB A:HIS115 3.4 14.8 1.0 CA A:HIS46 3.4 12.5 1.0 CB A:HIS46 3.5 12.0 1.0 CE A:MET120 3.7 14.2 1.0 C A:GLY45 4.0 14.1 1.0 NE2 A:HIS46 4.1 14.1 1.0 N A:HIS46 4.2 13.2 1.0 CD2 A:HIS46 4.2 15.1 1.0 NE2 A:HIS115 4.3 14.6 1.0 CD2 A:HIS115 4.3 15.1 1.0 N A:HIS115 4.3 14.0 1.0 CG A:PRO114 4.3 15.1 1.0 CA A:HIS115 4.5 14.0 1.0 N A:ASN47 4.5 11.0 1.0 C A:HIS46 4.5 12.6 1.0 CG A:MET120 4.6 15.1 1.0 CA A:CYS112 4.6 11.1 1.0 CD A:PRO114 4.8 13.4 1.0 CB A:MET120 5.0 14.2 1.0 C A:CYS112 5.0 11.4 1.0

Copper binding site 2 out of 2 in the Crystal Structure of Cu(I) Azurin with the Metal-Binding Loop Sequence "Ctfpghsalm" Replaced with "Csphqgagm", at PH4


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure of Cu(I) Azurin with the Metal-Binding Loop Sequence "Ctfpghsalm" Replaced with "Csphqgagm", at PH4 within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu201 b:15.0 occ:1.00 ND1 B:HIS115 2.1 13.9 1.0 ND1 B:HIS46 2.1 12.9 1.0 SG B:CYS112 2.2 11.5 1.0 CE1 B:HIS46 3.0 11.7 1.0 CG B:HIS115 3.0 12.1 1.0 CE1 B:HIS115 3.1 13.0 1.0 CG B:HIS46 3.1 13.3 1.0 O B:GLY45 3.2 12.6 1.0 SD B:MET120 3.2 12.6 1.0 CB B:HIS115 3.3 12.2 1.0 CA B:HIS46 3.3 12.7 1.0 CB B:CYS112 3.4 10.8 1.0 CB B:HIS46 3.5 13.1 1.0 CE B:MET120 3.8 16.0 1.0 C B:GLY45 4.0 14.1 1.0 CG B:PRO114 4.1 11.4 1.0 N B:HIS46 4.1 12.9 1.0 NE2 B:HIS46 4.2 12.2 1.0 CD2 B:HIS115 4.2 15.0 1.0 NE2 B:HIS115 4.2 14.6 1.0 N B:HIS115 4.2 11.4 1.0 CD2 B:HIS46 4.2 13.4 1.0 CA B:HIS115 4.4 11.2 1.0 C B:HIS46 4.5 11.7 1.0 N B:ASN47 4.6 11.3 1.0 CG B:MET120 4.7 11.5 1.0 CA B:CYS112 4.7 10.4 1.0 CD B:PRO114 4.8 12.3 1.0 CB B:MET120 4.9 11.3 1.0 CB B:PRO114 4.9 11.9 1.0

C.Li, M.J.Banfield, C.Dennison. Engineering Copper Sites in Proteins: Loops Confer Native Structures and Properties to Chimeric Cupredoxins. J.Am.Chem.Soc. V. 129 709 2007.
ISSN: ISSN 0002-7863
PubMed: 17227035
DOI: 10.1021/JA0661562