Copper in the structure of Crystal Structure of Cu(II) Azurin With the Metal-Binding Loop Sequence "Ctfpghsalm" Replaced With "Csphqgagm" (pdb 2hx7)

The binding sites of Copper atom in the structure of Crystal Structure of Cu(II) Azurin With the Metal-Binding Loop Sequence "Ctfpghsalm" Replaced With "Csphqgagm" (pdb code 2hx7). This binding sites where shown with 5.0 Angstroms radius around Copper atom. The 2hx7 structure was solved by M.J.BANFIELD, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 30.0-1.6 Space group P212121 a (A) 38.989 b (A) 65.276 c (A) 97.909 alpha (°) 90.00 beta (°) 90.00 gamma (°) 90.00 Rfactor (%) 14.4 Rfree (%) 19 Copper Binding Sites: Copper binding site 1 out of 2 in 2hx7 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Copper in the PDB 2hx7. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Gly45, A: His46, A: Asn47, A: Cys112, A: Pro114, A: His115, A: Met120, conact list: Atom Atom Distance (A) Cu O A:Gly45 3.08 Cu C A:Gly45 3.93 Cu NE2 A:His46 4.09 Cu N A:His46 4.11 Cu CB A:His46 3.49 Cu ND1 A:His46 2.03 Cu CD2 A:His46 4.18 Cu C A:His46 4.63 Cu CE1 A:His46 2.94 Cu CG A:His46 3.09 Cu CA A:His46 3.43 Cu N A:Asn47 4.68 Cu CB A:Cys112 3.23 Cu SG A:Cys112 2.16 Cu C A:Cys112 4.92 Cu CA A:Cys112 4.61 Cu CD A:Pro114 4.81 Cu CG A:Pro114 4.24 Cu NE2 A:His115 4.18 Cu N A:His115 4.25 Cu CB A:His115 3.33 Cu ND1 A:His115 2.09 Cu CD2 A:His115 4.18 Cu CE1 A:His115 3.08 Cu CG A:His115 3.03 Cu CA A:His115 4.42 Cu CB A:Met120 4.98 Cu CE A:Met120 3.83 Cu CG A:Met120 4.56 Cu SD A:Met120 3.13 interactive model: Copper binding site 2 out of 2 in 2hx7 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Copper in the PDB 2hx7. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: B: Gly45, B: His46, B: Asn47, B: Cys112, B: Pro114, B: His115, B: Met120, conact list: Atom Atom Distance (A) Cu O B:Gly45 3.20 Cu C B:Gly45 4.05 Cu NE2 B:His46 4.09 Cu N B:His46 4.19 Cu CB B:His46 3.55 Cu ND1 B:His46 2.05 Cu CD2 B:His46 4.20 Cu C B:His46 4.67 Cu CE1 B:His46 2.92 Cu CG B:His46 3.12 Cu CA B:His46 3.49 Cu N B:Asn47 4.66 Cu CB B:Cys112 3.27 Cu SG B:Cys112 2.21 Cu C B:Cys112 4.98 Cu CA B:Cys112 4.62 Cu CD B:Pro114 4.77 Cu CG B:Pro114 4.10 Cu NE2 B:His115 4.18 Cu N B:His115 4.33 Cu CB B:His115 3.33 Cu ND1 B:His115 2.08 Cu CD2 B:His115 4.18 Cu CE1 B:His115 3.08 Cu CG B:His115 3.04 Cu CA B:His115 4.45 Cu CB B:Met120 4.76 Cu CE B:Met120 3.79 Cu CG B:Met120 4.54 Cu SD B:Met120 3.10 interactive model: