Copper in the structure of Crystal Structure of Azurin PHE114PRO Mutant (pdb 2ghz)

The binding sites of Copper atom in the structure of Crystal Structure of Azurin PHE114PRO Mutant (pdb code 2ghz). This binding sites where shown with 5.0 Angstroms radius around Copper atom. The 2ghz structure was solved by S.YANAGISAWA, M.J.BANFIELD, C.DENNISON, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 38.8-1.6 Space group P212121 a (A) 35.350 b (A) 47.810 c (A) 132.520 alpha (°) 90.00 beta (°) 90.00 gamma (°) 90.00 Rfactor (%) 14.2 Rfree (%) 19 Copper Binding Sites: Copper binding site 1 out of 2 in 2ghz Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Copper in the PDB 2ghz. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Gly45, A: His46, A: Asn47, A: Cys112, A: Pro114, A: His117, A: Met121, conact list: Atom Atom Distance (A) Cu O A:Gly45 3.59 Cu C A:Gly45 4.37 Cu NE2 A:His46 4.08 Cu N A:His46 4.42 Cu CB A:His46 3.53 Cu ND1 A:His46 2.06 Cu CD2 A:His46 4.19 Cu C A:His46 4.70 Cu CE1 A:His46 2.93 Cu CG A:His46 3.12 Cu CA A:His46 3.58 Cu N A:Asn47 4.62 Cu CB A:Cys112 3.24 Cu SG A:Cys112 2.18 Cu C A:Cys112 4.84 Cu CA A:Cys112 4.56 Cu CB A:Pro114 4.58 Cu CD A:Pro114 4.19 Cu CG A:Pro114 4.08 Cu NE2 A:His117 4.06 Cu CB A:His117 3.36 Cu ND1 A:His117 1.97 Cu CD2 A:His117 4.12 Cu CE1 A:His117 2.93 Cu CG A:His117 3.00 Cu CA A:His117 4.81 Cu CB A:Met121 4.76 Cu CE A:Met121 3.60 Cu CG A:Met121 4.47 Cu SD A:Met121 2.99 interactive model: Copper binding site 2 out of 2 in 2ghz Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Copper in the PDB 2ghz. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: B: Gly45, B: His46, B: Asn47, B: Cys112, B: Pro114, B: His117, B: Met121, conact list: Atom Atom Distance (A) Cu O B:Gly45 3.60 Cu C B:Gly45 4.40 Cu NE2 B:His46 4.15 Cu N B:His46 4.42 Cu CB B:His46 3.52 Cu ND1 B:His46 2.11 Cu CD2 B:His46 4.23 Cu C B:His46 4.69 Cu CE1 B:His46 3.00 Cu CG B:His46 3.14 Cu CA B:His46 3.58 Cu N B:Asn47 4.59 Cu O B:Cys112 4.95 Cu CB B:Cys112 3.27 Cu SG B:Cys112 2.12 Cu C B:Cys112 4.78 Cu CA B:Cys112 4.54 Cu CB B:Pro114 4.65 Cu CD B:Pro114 4.17 Cu CG B:Pro114 4.14 Cu NE2 B:His117 4.07 Cu CB B:His117 3.36 Cu ND1 B:His117 1.99 Cu CD2 B:His117 4.12 Cu CE1 B:His117 2.94 Cu CG B:His117 3.01 Cu CA B:His117 4.78 Cu CB B:Met121 4.69 Cu CE B:Met121 3.50 Cu CG B:Met121 4.37 Cu SD B:Met121 2.85 interactive model: