Copper in PDB 2eil: Cadmium Ion Binding Structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State
Enzymatic activity of Cadmium Ion Binding Structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State
All present enzymatic activity of Cadmium Ion Binding Structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State:
1.9.3.1;
Protein crystallography data
The structure of Cadmium Ion Binding Structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State, PDB code: 2eil
was solved by
K.Muramoto,
K.Hirata,
K.Shinzawa-Itoh,
S.Yoko-O,
E.Yamashita,
H.Aoyama,
T.Tsukihara,
S.Yoshikawa,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
40.00 /
2.10
|
Space group
|
P 21 21 21
|
Cell size a, b, c (Å), α, β, γ (°)
|
183.379,
205.898,
178.182,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
19.9 /
23.1
|
Other elements in 2eil:
The structure of Cadmium Ion Binding Structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State also contains other interesting chemical elements:
Copper Binding Sites:
The binding sites of Copper atom in the Cadmium Ion Binding Structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State
(pdb code 2eil). This binding sites where shown within
5.0 Angstroms radius around Copper atom.
In total 6 binding sites of Copper where determined in the
Cadmium Ion Binding Structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State, PDB code: 2eil:
Jump to Copper binding site number:
1;
2;
3;
4;
5;
6;
Copper binding site 1 out
of 6 in 2eil
Go back to
Copper Binding Sites List in 2eil
Copper binding site 1 out
of 6 in the Cadmium Ion Binding Structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 1 of Cadmium Ion Binding Structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Cu517
b:22.5
occ:1.00
|
NE2
|
A:HIS291
|
1.9
|
28.4
|
1.0
|
ND1
|
A:HIS240
|
2.0
|
28.8
|
1.0
|
NE2
|
A:HIS290
|
2.0
|
19.5
|
1.0
|
CE1
|
A:HIS291
|
2.9
|
26.5
|
1.0
|
CE1
|
A:HIS240
|
3.0
|
24.5
|
1.0
|
CD2
|
A:HIS291
|
3.0
|
24.9
|
1.0
|
CE1
|
A:HIS290
|
3.0
|
18.0
|
1.0
|
CG
|
A:HIS240
|
3.1
|
25.1
|
1.0
|
CD2
|
A:HIS290
|
3.1
|
15.3
|
1.0
|
CB
|
A:HIS240
|
3.4
|
19.5
|
1.0
|
CA
|
A:HIS240
|
3.9
|
19.0
|
1.0
|
ND1
|
A:HIS291
|
4.1
|
24.0
|
1.0
|
CG
|
A:HIS291
|
4.1
|
25.5
|
1.0
|
NE2
|
A:HIS240
|
4.1
|
28.1
|
1.0
|
CD2
|
A:HIS240
|
4.2
|
25.4
|
1.0
|
ND1
|
A:HIS290
|
4.2
|
16.1
|
1.0
|
CG
|
A:HIS290
|
4.3
|
17.9
|
1.0
|
NA
|
A:HEA516
|
4.5
|
20.6
|
1.0
|
C1A
|
A:HEA516
|
4.6
|
17.1
|
1.0
|
CG2
|
A:VAL243
|
4.7
|
15.6
|
1.0
|
N
|
A:HIS240
|
4.8
|
17.0
|
1.0
|
C4A
|
A:HEA516
|
4.8
|
20.5
|
1.0
|
FE
|
A:HEA516
|
4.8
|
19.6
|
1.0
|
C2A
|
A:HEA516
|
4.9
|
19.5
|
1.0
|
CHA
|
A:HEA516
|
4.9
|
18.0
|
1.0
|
C3A
|
A:HEA516
|
5.0
|
18.5
|
1.0
|
|
Copper binding site 2 out
of 6 in 2eil
Go back to
Copper Binding Sites List in 2eil
Copper binding site 2 out
of 6 in the Cadmium Ion Binding Structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 2 of Cadmium Ion Binding Structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Cu228
b:24.0
occ:1.00
|
CU1
|
B:CUA228
|
0.0
|
24.0
|
1.0
|
ND1
|
B:HIS161
|
2.0
|
17.9
|
1.0
|
SG
|
B:CYS196
|
2.3
|
20.3
|
1.0
|
SG
|
B:CYS200
|
2.3
|
20.9
|
1.0
|
CU2
|
B:CUA228
|
2.6
|
23.1
|
1.0
|
SD
|
B:MET207
|
2.7
|
24.6
|
1.0
|
CE1
|
B:HIS161
|
2.9
|
16.1
|
1.0
|
CE
|
B:MET207
|
3.0
|
11.4
|
1.0
|
CG
|
B:HIS161
|
3.2
|
16.0
|
1.0
|
CB
|
B:CYS200
|
3.3
|
22.2
|
1.0
|
CB
|
B:CYS196
|
3.4
|
19.4
|
1.0
|
CG
|
B:MET207
|
3.6
|
20.9
|
1.0
|
CB
|
B:HIS161
|
3.7
|
21.4
|
1.0
|
O
|
B:GLU198
|
4.1
|
30.1
|
1.0
|
NE2
|
B:HIS161
|
4.1
|
15.5
|
1.0
|
CA
|
B:HIS161
|
4.2
|
18.7
|
1.0
|
CD2
|
B:HIS161
|
4.3
|
15.3
|
1.0
|
ND1
|
B:HIS204
|
4.4
|
24.6
|
1.0
|
O
|
B:LEU160
|
4.6
|
21.4
|
1.0
|
CA
|
B:CYS200
|
4.7
|
25.0
|
1.0
|
O
|
B:HIS102
|
4.7
|
19.6
|
1.0
|
CD1
|
B:TRP104
|
4.7
|
23.6
|
1.0
|
CA
|
B:CYS196
|
4.7
|
22.1
|
1.0
|
CA
|
B:HIS204
|
4.8
|
24.9
|
1.0
|
N
|
B:CYS200
|
4.9
|
24.7
|
1.0
|
O
|
B:HIS204
|
5.0
|
20.5
|
1.0
|
|
Copper binding site 3 out
of 6 in 2eil
Go back to
Copper Binding Sites List in 2eil
Copper binding site 3 out
of 6 in the Cadmium Ion Binding Structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 3 of Cadmium Ion Binding Structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Cu228
b:23.1
occ:1.00
|
CU2
|
B:CUA228
|
0.0
|
23.1
|
1.0
|
ND1
|
B:HIS204
|
1.9
|
24.6
|
1.0
|
SG
|
B:CYS200
|
2.2
|
20.9
|
1.0
|
SG
|
B:CYS196
|
2.3
|
20.3
|
1.0
|
O
|
B:GLU198
|
2.4
|
30.1
|
1.0
|
CU1
|
B:CUA228
|
2.6
|
24.0
|
1.0
|
CE1
|
B:HIS204
|
2.8
|
19.2
|
1.0
|
CG
|
B:HIS204
|
3.0
|
22.4
|
1.0
|
CB
|
B:CYS196
|
3.3
|
19.4
|
1.0
|
CB
|
B:CYS200
|
3.5
|
22.2
|
1.0
|
CB
|
B:HIS204
|
3.5
|
22.4
|
1.0
|
C
|
B:GLU198
|
3.5
|
24.6
|
1.0
|
CA
|
B:HIS204
|
3.5
|
24.9
|
1.0
|
N
|
B:CYS200
|
3.6
|
24.7
|
1.0
|
O
|
B:HIS204
|
3.7
|
20.5
|
1.0
|
NE2
|
B:HIS204
|
4.0
|
24.4
|
1.0
|
C
|
B:HIS204
|
4.1
|
22.2
|
1.0
|
O
|
B:CYS196
|
4.1
|
21.1
|
1.0
|
CD2
|
B:HIS204
|
4.1
|
22.4
|
1.0
|
N
|
B:GLU198
|
4.1
|
23.3
|
1.0
|
C
|
B:ILE199
|
4.2
|
23.2
|
1.0
|
CA
|
B:CYS200
|
4.2
|
25.0
|
1.0
|
C
|
B:CYS196
|
4.2
|
24.6
|
1.0
|
CA
|
B:ILE199
|
4.2
|
20.6
|
1.0
|
ND1
|
B:HIS161
|
4.2
|
17.9
|
1.0
|
N
|
B:ILE199
|
4.3
|
24.8
|
1.0
|
CA
|
B:CYS196
|
4.3
|
22.1
|
1.0
|
CA
|
B:GLU198
|
4.5
|
22.0
|
1.0
|
SD
|
B:MET207
|
4.5
|
24.6
|
1.0
|
CG
|
B:MET207
|
4.7
|
20.9
|
1.0
|
N
|
B:SER197
|
4.8
|
22.9
|
1.0
|
N
|
B:HIS204
|
4.8
|
28.2
|
1.0
|
CA
|
B:HIS161
|
5.0
|
18.7
|
1.0
|
|
Copper binding site 4 out
of 6 in 2eil
Go back to
Copper Binding Sites List in 2eil
Copper binding site 4 out
of 6 in the Cadmium Ion Binding Structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 4 of Cadmium Ion Binding Structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
N:Cu517
b:27.1
occ:1.00
|
NE2
|
N:HIS291
|
2.0
|
29.1
|
1.0
|
ND1
|
N:HIS240
|
2.0
|
25.4
|
1.0
|
NE2
|
N:HIS290
|
2.0
|
19.8
|
1.0
|
CE1
|
N:HIS291
|
2.9
|
30.7
|
1.0
|
CE1
|
N:HIS290
|
2.9
|
25.0
|
1.0
|
CE1
|
N:HIS240
|
2.9
|
24.0
|
1.0
|
CD2
|
N:HIS291
|
3.0
|
29.4
|
1.0
|
CG
|
N:HIS240
|
3.1
|
27.3
|
1.0
|
CD2
|
N:HIS290
|
3.1
|
21.7
|
1.0
|
CB
|
N:HIS240
|
3.4
|
26.4
|
1.0
|
CA
|
N:HIS240
|
3.9
|
26.2
|
1.0
|
ND1
|
N:HIS291
|
4.1
|
28.9
|
1.0
|
NE2
|
N:HIS240
|
4.1
|
27.9
|
1.0
|
CG
|
N:HIS291
|
4.1
|
28.4
|
1.0
|
ND1
|
N:HIS290
|
4.1
|
21.3
|
1.0
|
CD2
|
N:HIS240
|
4.2
|
27.6
|
1.0
|
CG
|
N:HIS290
|
4.2
|
23.5
|
1.0
|
NA
|
N:HEA516
|
4.5
|
25.4
|
1.0
|
C1A
|
N:HEA516
|
4.7
|
24.1
|
1.0
|
N
|
N:HIS240
|
4.7
|
24.0
|
1.0
|
CG2
|
N:VAL243
|
4.7
|
21.5
|
1.0
|
C4A
|
N:HEA516
|
4.8
|
26.3
|
1.0
|
FE
|
N:HEA516
|
4.9
|
24.1
|
1.0
|
C2A
|
N:HEA516
|
4.9
|
24.5
|
1.0
|
CHA
|
N:HEA516
|
4.9
|
22.8
|
1.0
|
ND
|
N:HEA516
|
5.0
|
24.3
|
1.0
|
C3A
|
N:HEA516
|
5.0
|
20.5
|
1.0
|
|
Copper binding site 5 out
of 6 in 2eil
Go back to
Copper Binding Sites List in 2eil
Copper binding site 5 out
of 6 in the Cadmium Ion Binding Structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 5 of Cadmium Ion Binding Structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
O:Cu228
b:32.8
occ:1.00
|
CU1
|
O:CUA228
|
0.0
|
32.8
|
1.0
|
ND1
|
O:HIS161
|
2.0
|
32.8
|
1.0
|
SG
|
O:CYS196
|
2.3
|
27.3
|
1.0
|
SG
|
O:CYS200
|
2.3
|
29.7
|
1.0
|
CU2
|
O:CUA228
|
2.4
|
32.3
|
1.0
|
SD
|
O:MET207
|
2.7
|
34.0
|
1.0
|
CE1
|
O:HIS161
|
2.8
|
29.1
|
1.0
|
CE
|
O:MET207
|
3.1
|
22.2
|
1.0
|
CG
|
O:HIS161
|
3.2
|
28.6
|
1.0
|
CB
|
O:CYS196
|
3.3
|
34.8
|
1.0
|
CB
|
O:CYS200
|
3.3
|
33.1
|
1.0
|
CG
|
O:MET207
|
3.7
|
33.2
|
1.0
|
CB
|
O:HIS161
|
3.7
|
30.1
|
1.0
|
NE2
|
O:HIS161
|
4.0
|
27.8
|
1.0
|
O
|
O:GLU198
|
4.1
|
32.2
|
1.0
|
CD2
|
O:HIS161
|
4.2
|
27.0
|
1.0
|
CA
|
O:HIS161
|
4.2
|
28.8
|
1.0
|
ND1
|
O:HIS204
|
4.3
|
32.9
|
1.0
|
CD1
|
O:TRP104
|
4.7
|
34.4
|
1.0
|
CA
|
O:CYS200
|
4.7
|
33.0
|
1.0
|
CA
|
O:HIS204
|
4.7
|
30.8
|
1.0
|
CA
|
O:CYS196
|
4.7
|
33.3
|
1.0
|
O
|
O:LEU160
|
4.7
|
28.6
|
1.0
|
O
|
O:HIS102
|
4.8
|
26.6
|
1.0
|
O
|
O:HIS204
|
4.9
|
29.0
|
1.0
|
N
|
O:CYS200
|
4.9
|
34.3
|
1.0
|
|
Copper binding site 6 out
of 6 in 2eil
Go back to
Copper Binding Sites List in 2eil
Copper binding site 6 out
of 6 in the Cadmium Ion Binding Structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 6 of Cadmium Ion Binding Structure of Bovine Heart Cytochrome C Oxidase in the Fully Oxidized State within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
O:Cu228
b:32.3
occ:1.00
|
CU2
|
O:CUA228
|
0.0
|
32.3
|
1.0
|
ND1
|
O:HIS204
|
2.1
|
32.9
|
1.0
|
SG
|
O:CYS196
|
2.2
|
27.3
|
1.0
|
SG
|
O:CYS200
|
2.3
|
29.7
|
1.0
|
CU1
|
O:CUA228
|
2.4
|
32.8
|
1.0
|
O
|
O:GLU198
|
2.4
|
32.2
|
1.0
|
CE1
|
O:HIS204
|
2.9
|
29.0
|
1.0
|
CG
|
O:HIS204
|
3.2
|
29.8
|
1.0
|
CB
|
O:CYS196
|
3.2
|
34.8
|
1.0
|
CB
|
O:CYS200
|
3.4
|
33.1
|
1.0
|
C
|
O:GLU198
|
3.5
|
25.9
|
1.0
|
N
|
O:CYS200
|
3.6
|
34.3
|
1.0
|
CA
|
O:HIS204
|
3.6
|
30.8
|
1.0
|
CB
|
O:HIS204
|
3.7
|
30.0
|
1.0
|
O
|
O:HIS204
|
3.9
|
29.0
|
1.0
|
ND1
|
O:HIS161
|
3.9
|
32.8
|
1.0
|
NE2
|
O:HIS204
|
4.1
|
28.5
|
1.0
|
CA
|
O:CYS200
|
4.1
|
33.0
|
1.0
|
N
|
O:GLU198
|
4.1
|
29.9
|
1.0
|
O
|
O:CYS196
|
4.2
|
30.0
|
1.0
|
C
|
O:ILE199
|
4.2
|
32.3
|
1.0
|
C
|
O:HIS204
|
4.2
|
27.0
|
1.0
|
CD2
|
O:HIS204
|
4.2
|
26.4
|
1.0
|
C
|
O:CYS196
|
4.3
|
32.9
|
1.0
|
CA
|
O:ILE199
|
4.3
|
28.4
|
1.0
|
N
|
O:ILE199
|
4.3
|
27.3
|
1.0
|
CA
|
O:CYS196
|
4.4
|
33.3
|
1.0
|
CA
|
O:GLU198
|
4.5
|
26.3
|
1.0
|
SD
|
O:MET207
|
4.5
|
34.0
|
1.0
|
CE1
|
O:HIS161
|
4.7
|
29.1
|
1.0
|
CG
|
O:MET207
|
4.8
|
33.2
|
1.0
|
N
|
O:SER197
|
4.8
|
31.5
|
1.0
|
N
|
O:HIS204
|
4.9
|
31.4
|
1.0
|
CA
|
O:HIS161
|
4.9
|
28.8
|
1.0
|
CG
|
O:HIS161
|
5.0
|
28.6
|
1.0
|
|
Reference:
K.Muramoto,
K.Hirata,
K.Shinzawa-Itoh,
S.Yoko-O,
E.Yamashita,
H.Aoyama,
T.Tsukihara,
S.Yoshikawa.
A Histidine Residue Acting As A Controlling Site For Dioxygen Reduction and Proton Pumping By Cytochrome C Oxidase Proc.Natl.Acad.Sci.Usa V. 104 7881 2007.
ISSN: ISSN 0027-8424
PubMed: 17470809
DOI: 10.1073/PNAS.0610031104
Page generated: Tue Jul 30 23:26:58 2024
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