Copper in the structure of Galactose Oxidase W290G Mutant (pdb 2eid)

The binding sites of Copper atom in the structure of Galactose Oxidase W290G Mutant (pdb code 2eid). This binding sites where shown with 5.0 Angstroms radius around Copper atom. The 2eid structure was solved by S.E.PHILLIPS, M.J.MCPHERSON, P.F.KNOWLES, N.AKYUMANI, S.J.FIRBANK, S.TAMBER, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 10.0-2.2 Space group P6522 a (A) 89.884 b (A) 89.884 c (A) 415.325 alpha (°) 90.00 beta (°) 90.00 gamma (°) 120.00 Rfactor (%) 19.2 Rfree (%) 22.6 Copper Binding Sites: Copper binding site 1 out of 1 in 2eid Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Copper in the PDB 2eid. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Phe227, A: Cys228, A: Tyr272, A: Tyr405, A: Tyr495, A: His496, A: His581, A: Hoh993, conact list: Atom Atom Distance (A) Cu CE2 A:Phe227 4.14 Cu CZ A:Phe227 3.26 Cu CE1 A:Phe227 3.88 Cu CB A:Cys228 5.00 Cu SG A:Cys228 3.30 Cu CE2 A:Tyr272 3.92 Cu CD1 A:Tyr272 4.74 Cu CZ A:Tyr272 2.92 Cu CE1 A:Tyr272 3.46 Cu OH A:Tyr272 2.04 Cu OH A:Tyr405 4.98 Cu CE2 A:Tyr495 3.87 Cu CD2 A:Tyr495 4.98 Cu CZ A:Tyr495 3.43 Cu CE1 A:Tyr495 4.27 Cu OH A:Tyr495 2.85 Cu NE2 A:His496 2.27 Cu ND1 A:His496 4.39 Cu CD2 A:His496 3.16 Cu CE1 A:His496 3.31 Cu CG A:His496 4.36 Cu NE2 A:His581 2.41 Cu ND1 A:His581 4.48 Cu CD2 A:His581 2.96 Cu CE1 A:His581 3.58 Cu CG A:His581 4.22 Cu O A:Hoh993 4.70 interactive model: