Copper in the structure of Crystal Structure of Galactose Oxidase Mutant W290F (pdb 2eic)

The binding sites of Copper atom in the structure of Crystal Structure of Galactose Oxidase Mutant W290F (pdb code 2eic). This binding sites where shown with 5.0 Angstroms radius around Copper atom. The 2eic structure was solved by N.AKYUMANI, S.TAMBER, S.J.FIRBANK, P.F.KNOWLES, S.E.PHILLIPS, M.J.MCPHERSON, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 30.0-2.8 Space group C121 a (A) 97.770 b (A) 88.890 c (A) 86.190 alpha (°) 90.00 beta (°) 117.90 gamma (°) 90.00 Rfactor (%) 17.7 Rfree (%) 23.3 Copper Binding Sites: Copper binding site 1 out of 1 in 2eic Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Copper in the PDB 2eic. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Phe227, A: Cys228, A: Tyr272, A: Tyr495, A: His496, A: His581, A: Hoh804, conact list: Atom Atom Distance (A) Cu CE2 A:Phe227 4.07 Cu CZ A:Phe227 3.30 Cu CE1 A:Phe227 3.98 Cu SG A:Cys228 3.45 Cu CE2 A:Tyr272 3.97 Cu CD1 A:Tyr272 4.66 Cu CZ A:Tyr272 2.92 Cu CE1 A:Tyr272 3.40 Cu OH A:Tyr272 2.07 Cu CE2 A:Tyr495 3.56 Cu CD2 A:Tyr495 4.76 Cu CZ A:Tyr495 3.33 Cu CE1 A:Tyr495 4.42 Cu OH A:Tyr495 2.62 Cu NE2 A:His496 2.17 Cu ND1 A:His496 4.25 Cu CD2 A:His496 3.16 Cu CE1 A:His496 3.13 Cu CG A:His496 4.30 Cu NE2 A:His581 2.21 Cu ND1 A:His581 4.30 Cu CD2 A:His581 3.07 Cu CE1 A:His581 3.25 Cu CG A:His581 4.25 Cu O A:Hoh804 4.58 interactive model: