Copper in PDB 2dws: Cu-Containing Nitrite Reductase at pH 8.4 with Bound Nitrite

All present enzymatic activity of Cu-Containing Nitrite Reductase at pH 8.4 with Bound Nitrite:
1.7.2.1;

The structure of Cu-Containing Nitrite Reductase at pH 8.4 with Bound Nitrite, PDB code: 2dws was solved by F.Jacobson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	26.46 / 1.85
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	82.204, 82.204, 146.152, 90.00, 90.00, 120.00
R / Rfree (%)	16.8 / 19.9

The binding sites of Copper atom in the Cu-Containing Nitrite Reductase at pH 8.4 with Bound Nitrite (pdb code 2dws). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Cu-Containing Nitrite Reductase at pH 8.4 with Bound Nitrite, PDB code: 2dws:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the Cu-Containing Nitrite Reductase at pH 8.4 with Bound Nitrite


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Cu-Containing Nitrite Reductase at pH 8.4 with Bound Nitrite within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu401 b:25.7 occ:1.00 ND1 A:HIS177 2.0 22.6 1.0 ND1 A:HIS126 2.1 23.7 1.0 SG A:CYS167 2.2 25.5 1.0 SD A:MET182 2.4 24.9 1.0 CE1 A:HIS177 3.0 26.0 1.0 CE1 A:HIS126 3.1 28.0 1.0 CG A:HIS126 3.1 27.2 1.0 CG A:HIS177 3.1 22.0 1.0 CB A:CYS167 3.1 22.9 1.0 CE A:MET182 3.2 24.1 1.0 CB A:HIS126 3.4 25.9 1.0 CB A:HIS177 3.5 20.0 1.0 CG A:MET182 3.8 25.3 1.0 CA A:HIS126 3.9 25.8 1.0 NE2 A:HIS177 4.1 25.5 1.0 CD2 A:HIS177 4.2 22.7 1.0 NE2 A:HIS126 4.2 23.6 1.0 CD2 A:HIS126 4.2 25.1 1.0 CG A:PRO169 4.3 26.1 1.0 CB A:MET182 4.4 20.3 1.0 O A:PRO125 4.4 26.4 1.0 SD A:MET93 4.5 23.1 0.5 CA A:CYS167 4.6 22.7 1.0 N A:ASN127 4.7 24.4 1.0 CA A:HIS177 4.8 19.8 1.0 CD A:PRO169 4.8 25.5 1.0 C A:HIS126 4.8 25.7 1.0 N A:HIS126 4.9 27.3 1.0 CB A:MET93 4.9 23.1 0.5

Copper binding site 2 out of 2 in the Cu-Containing Nitrite Reductase at pH 8.4 with Bound Nitrite


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Cu-Containing Nitrite Reductase at pH 8.4 with Bound Nitrite within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu402 b:25.8 occ:1.00 NE2 A:HIS131 2.0 22.4 1.0 NE2 A:HIS166 2.1 21.4 1.0 CE1 A:HIS131 2.9 20.9 1.0 CD2 A:HIS166 3.0 22.4 1.0 CD2 A:HIS131 3.1 20.8 1.0 CE1 A:HIS166 3.1 23.1 1.0 OD2 A:ASP129 3.6 33.9 1.0 ND1 A:HIS131 4.0 20.0 1.0 CG A:HIS131 4.2 18.6 1.0 CG A:HIS166 4.2 22.6 1.0 ND1 A:HIS166 4.2 22.4 1.0 CG A:ASP129 4.3 29.6 1.0 OD1 A:ASP129 4.6 27.6 1.0 O A:HOH582 4.7 27.7 1.0

F.Jacobson, A.Pistorius, D.Farkas, W.De Grip, O.Hansson, L.Sjolin, R.Neutze. pH Dependence of Copper Geometry, Reduction Potential, and Nitrite Affinity in Nitrite Reductase J.Biol.Chem. V. 282 6347 2007.
ISSN: ISSN 0021-9258
PubMed: 17148448
DOI: 10.1074/JBC.M605746200