Copper in PDB 2bwd: Atomic Resolution Structure of Achromobacter Cycloclastes Cu Nitrite Reductase with Endogenously Bound Nitrite and No

All present enzymatic activity of Atomic Resolution Structure of Achromobacter Cycloclastes Cu Nitrite Reductase with Endogenously Bound Nitrite and No:
1.7.2.1;

The structure of Atomic Resolution Structure of Achromobacter Cycloclastes Cu Nitrite Reductase with Endogenously Bound Nitrite and No, PDB code: 2bwd was solved by S.V.Antonyuk, R.W.Strange, G.Sawers, R.R.Eady, S.S.Hasnain, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.15
Space group	P 21 3
Cell size a, b, c (Å), α, β, γ (°)	96.237, 96.237, 96.237, 90.00, 90.00, 90.00
R / Rfree (%)	11.3 / 14.4

The binding sites of Copper atom in the Atomic Resolution Structure of Achromobacter Cycloclastes Cu Nitrite Reductase with Endogenously Bound Nitrite and No (pdb code 2bwd). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Atomic Resolution Structure of Achromobacter Cycloclastes Cu Nitrite Reductase with Endogenously Bound Nitrite and No, PDB code: 2bwd:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the Atomic Resolution Structure of Achromobacter Cycloclastes Cu Nitrite Reductase with Endogenously Bound Nitrite and No


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Atomic Resolution Structure of Achromobacter Cycloclastes Cu Nitrite Reductase with Endogenously Bound Nitrite and No within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu501 b:7.2 occ:1.00 ND1 A:HIS145 2.0 6.4 1.0 ND1 A:HIS95 2.0 6.9 1.0 SG A:CYS136 2.2 7.7 1.0 SD A:MET150 2.5 6.8 1.0 CE1 A:HIS145 2.9 7.3 1.0 CE1 A:HIS95 3.0 7.0 1.0 CG A:HIS95 3.1 6.9 1.0 CG A:HIS145 3.1 6.4 1.0 CB A:CYS136 3.2 6.7 1.0 CE A:MET150 3.3 7.7 1.0 CB A:HIS95 3.5 7.2 1.0 CB A:HIS145 3.6 6.6 1.0 CA A:HIS95 3.9 7.8 1.0 CG A:MET150 3.9 6.2 1.0 NE2 A:HIS145 4.1 8.8 1.0 NE2 A:HIS95 4.2 7.5 1.0 CD2 A:HIS145 4.2 8.2 1.0 CD2 A:HIS95 4.2 6.8 1.0 O A:LEU94 4.3 9.4 1.0 CG A:PRO138 4.3 7.7 1.0 CB A:MET150 4.4 6.6 1.0 SD A:MET62 4.4 8.6 1.0 CA A:CYS136 4.7 6.4 1.0 N A:ASN96 4.7 7.2 1.0 CD A:PRO138 4.7 7.8 1.0 CA A:HIS145 4.8 6.2 1.0 C A:HIS95 4.9 6.9 1.0 CB A:MET62 4.9 7.6 1.0 N A:HIS95 4.9 7.5 1.0

Copper binding site 2 out of 2 in the Atomic Resolution Structure of Achromobacter Cycloclastes Cu Nitrite Reductase with Endogenously Bound Nitrite and No


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Atomic Resolution Structure of Achromobacter Cycloclastes Cu Nitrite Reductase with Endogenously Bound Nitrite and No within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu502 b:6.8 occ:0.80 N A:HOA506 2.0 6.8 0.3 O A:HOA506 2.0 10.9 0.3 NE2 A:HIS100 2.1 5.8 1.0 NE2 A:HIS135 2.1 7.5 1.0 N A:NO2507 2.1 8.8 0.5 O2 A:NO2507 2.1 13.3 0.5 O1 A:NO2507 2.1 12.1 0.5 CE1 A:HIS100 3.0 6.6 1.0 CE1 A:HIS135 3.1 7.3 1.0 CD2 A:HIS135 3.1 7.7 1.0 CD2 A:HIS100 3.1 5.0 1.0 O A:HOH2238 3.8 16.3 0.5 OD2 A:ASP98 3.9 13.6 0.6 ND1 A:HIS100 4.2 5.5 1.0 O A:HOH2563 4.2 14.0 0.5 ND1 A:HIS135 4.2 6.7 1.0 CG A:HIS100 4.2 5.3 1.0 CG A:HIS135 4.3 5.8 1.0 O A:HOH2235 4.3 12.8 0.5 CG A:ASP98 4.6 10.6 0.6 OD1 A:ASP98 4.8 10.8 0.6

S.V.Antonyuk, R.W.Strange, G.Sawers, R.R.Eady, S.S.Hasnain. Atomic Resolution Structures of Resting-State, Substrate- and Product-Complexed Cu-Nitrite Reductase Provide Insight Into Catalytic Mechanism Proc.Natl.Acad.Sci.Usa V. 102 12041 2005.
ISSN: ISSN 0027-8424
PubMed: 16093314
DOI: 10.1073/PNAS.0504207102