Copper in PDB 2azu: X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa
Protein crystallography data
The structure of X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa, PDB code: 2azu
was solved by
H.Nar,
A.Messerschmidt,
R.Huber,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
N/A /
1.90
|
Space group
|
P 21 21 21
|
Cell size a, b, c (Å), α, β, γ (°)
|
57.820,
81.060,
110.030,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
17 /
n/a
|
Copper Binding Sites:
The binding sites of Copper atom in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa
(pdb code 2azu). This binding sites where shown within
5.0 Angstroms radius around Copper atom.
In total 4 binding sites of Copper where determined in the
X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa, PDB code: 2azu:
Jump to Copper binding site number:
1;
2;
3;
4;
Copper binding site 1 out
of 4 in 2azu
Go back to
Copper Binding Sites List in 2azu
Copper binding site 1 out
of 4 in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 1 of X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Cu129
b:14.4
occ:1.00
|
ND1
|
A:HIS46
|
2.1
|
24.1
|
1.0
|
ND1
|
A:HIS117
|
2.1
|
9.6
|
1.0
|
SG
|
A:CYS112
|
2.2
|
8.4
|
1.0
|
SD
|
A:MET121
|
3.0
|
14.0
|
1.0
|
CE1
|
A:HIS46
|
3.0
|
8.0
|
1.0
|
CE1
|
A:HIS117
|
3.0
|
4.0
|
1.0
|
CG
|
A:HIS117
|
3.1
|
9.0
|
1.0
|
CG
|
A:HIS46
|
3.1
|
10.4
|
1.0
|
O
|
A:GLY45
|
3.1
|
19.1
|
1.0
|
CB
|
A:CYS112
|
3.2
|
2.0
|
1.0
|
CB
|
A:HIS117
|
3.4
|
2.0
|
1.0
|
CA
|
A:HIS46
|
3.5
|
6.7
|
1.0
|
CB
|
A:HIS46
|
3.5
|
10.6
|
1.0
|
C
|
A:GLY45
|
4.0
|
36.0
|
1.0
|
CB
|
A:PHE114
|
4.0
|
4.2
|
1.0
|
CE
|
A:MET121
|
4.1
|
4.4
|
1.0
|
NE2
|
A:HIS46
|
4.1
|
8.5
|
1.0
|
NE2
|
A:HIS117
|
4.2
|
7.6
|
1.0
|
N
|
A:HIS46
|
4.2
|
10.9
|
1.0
|
CD2
|
A:HIS117
|
4.2
|
7.9
|
1.0
|
CD2
|
A:HIS46
|
4.3
|
14.7
|
1.0
|
CG
|
A:MET121
|
4.5
|
17.9
|
1.0
|
CA
|
A:CYS112
|
4.6
|
4.3
|
1.0
|
C
|
A:HIS46
|
4.7
|
3.0
|
1.0
|
N
|
A:ASN47
|
4.7
|
5.5
|
1.0
|
CA
|
A:HIS117
|
4.9
|
16.2
|
1.0
|
CG
|
A:PHE114
|
4.9
|
9.6
|
1.0
|
CB
|
A:MET121
|
4.9
|
15.9
|
1.0
|
N
|
A:PHE114
|
4.9
|
15.8
|
1.0
|
|
Copper binding site 2 out
of 4 in 2azu
Go back to
Copper Binding Sites List in 2azu
Copper binding site 2 out
of 4 in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 2 of X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Cu129
b:13.0
occ:1.00
|
ND1
|
B:HIS117
|
2.0
|
10.7
|
1.0
|
ND1
|
B:HIS46
|
2.1
|
18.2
|
1.0
|
SG
|
B:CYS112
|
2.2
|
8.4
|
1.0
|
CE1
|
B:HIS117
|
3.0
|
14.7
|
1.0
|
O
|
B:GLY45
|
3.0
|
9.7
|
1.0
|
CE1
|
B:HIS46
|
3.1
|
19.6
|
1.0
|
CG
|
B:HIS46
|
3.1
|
21.0
|
1.0
|
CG
|
B:HIS117
|
3.1
|
12.1
|
1.0
|
SD
|
B:MET121
|
3.1
|
13.3
|
1.0
|
CB
|
B:CYS112
|
3.2
|
2.0
|
1.0
|
CA
|
B:HIS46
|
3.4
|
2.0
|
1.0
|
CB
|
B:HIS46
|
3.4
|
12.4
|
1.0
|
CB
|
B:HIS117
|
3.5
|
10.2
|
1.0
|
CB
|
B:PHE114
|
3.8
|
17.7
|
1.0
|
CE
|
B:MET121
|
3.9
|
7.2
|
1.0
|
C
|
B:GLY45
|
4.0
|
5.8
|
1.0
|
NE2
|
B:HIS117
|
4.1
|
9.4
|
1.0
|
N
|
B:HIS46
|
4.2
|
5.5
|
1.0
|
NE2
|
B:HIS46
|
4.2
|
16.6
|
1.0
|
CD2
|
B:HIS117
|
4.2
|
8.7
|
1.0
|
CD2
|
B:HIS46
|
4.2
|
8.7
|
1.0
|
C
|
B:HIS46
|
4.6
|
10.6
|
1.0
|
CG
|
B:PHE114
|
4.6
|
9.3
|
1.0
|
CA
|
B:CYS112
|
4.6
|
4.2
|
1.0
|
CG
|
B:MET121
|
4.6
|
14.0
|
1.0
|
N
|
B:ASN47
|
4.7
|
2.0
|
1.0
|
N
|
B:PHE114
|
4.8
|
10.9
|
1.0
|
CA
|
B:PHE114
|
4.9
|
12.9
|
1.0
|
CB
|
B:MET121
|
5.0
|
3.1
|
1.0
|
CA
|
B:HIS117
|
5.0
|
14.3
|
1.0
|
|
Copper binding site 3 out
of 4 in 2azu
Go back to
Copper Binding Sites List in 2azu
Copper binding site 3 out
of 4 in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 3 of X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Cu129
b:12.2
occ:1.00
|
ND1
|
C:HIS117
|
2.0
|
6.6
|
1.0
|
ND1
|
C:HIS46
|
2.1
|
8.7
|
1.0
|
SG
|
C:CYS112
|
2.2
|
7.4
|
1.0
|
CE1
|
C:HIS117
|
3.0
|
2.9
|
1.0
|
CG
|
C:HIS117
|
3.0
|
19.8
|
1.0
|
SD
|
C:MET121
|
3.1
|
12.4
|
1.0
|
CE1
|
C:HIS46
|
3.1
|
12.5
|
1.0
|
CG
|
C:HIS46
|
3.1
|
13.9
|
1.0
|
O
|
C:GLY45
|
3.1
|
16.5
|
1.0
|
CB
|
C:CYS112
|
3.2
|
5.2
|
1.0
|
CB
|
C:HIS117
|
3.4
|
16.2
|
1.0
|
CA
|
C:HIS46
|
3.4
|
4.5
|
1.0
|
CB
|
C:HIS46
|
3.5
|
4.8
|
1.0
|
CE
|
C:MET121
|
3.9
|
3.4
|
1.0
|
CB
|
C:PHE114
|
3.9
|
5.9
|
1.0
|
C
|
C:GLY45
|
4.0
|
12.4
|
1.0
|
NE2
|
C:HIS117
|
4.1
|
14.3
|
1.0
|
CD2
|
C:HIS117
|
4.2
|
7.8
|
1.0
|
NE2
|
C:HIS46
|
4.2
|
14.9
|
1.0
|
N
|
C:HIS46
|
4.2
|
8.6
|
1.0
|
CD2
|
C:HIS46
|
4.3
|
14.5
|
1.0
|
C
|
C:HIS46
|
4.6
|
12.2
|
1.0
|
N
|
C:ASN47
|
4.6
|
5.4
|
1.0
|
CG
|
C:MET121
|
4.6
|
8.5
|
1.0
|
CA
|
C:CYS112
|
4.7
|
2.0
|
1.0
|
CG
|
C:PHE114
|
4.8
|
8.4
|
1.0
|
N
|
C:PHE114
|
4.8
|
16.9
|
1.0
|
CA
|
C:HIS117
|
4.9
|
12.2
|
1.0
|
CA
|
C:PHE114
|
5.0
|
7.3
|
1.0
|
CB
|
C:MET121
|
5.0
|
8.9
|
1.0
|
|
Copper binding site 4 out
of 4 in 2azu
Go back to
Copper Binding Sites List in 2azu
Copper binding site 4 out
of 4 in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa
Mono view
Stereo pair view
|
A full contact list of Copper with other atoms in the Cu binding
site number 4 of X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
D:Cu129
b:13.1
occ:1.00
|
ND1
|
D:HIS117
|
2.0
|
5.2
|
1.0
|
ND1
|
D:HIS46
|
2.1
|
18.3
|
1.0
|
SG
|
D:CYS112
|
2.2
|
8.8
|
1.0
|
SD
|
D:MET121
|
2.9
|
13.2
|
1.0
|
CE1
|
D:HIS117
|
2.9
|
2.0
|
1.0
|
CG
|
D:HIS117
|
2.9
|
6.1
|
1.0
|
CE1
|
D:HIS46
|
3.1
|
10.0
|
1.0
|
O
|
D:GLY45
|
3.1
|
12.9
|
1.0
|
CG
|
D:HIS46
|
3.1
|
13.8
|
1.0
|
CB
|
D:CYS112
|
3.3
|
5.0
|
1.0
|
CB
|
D:HIS117
|
3.3
|
5.4
|
1.0
|
CA
|
D:HIS46
|
3.4
|
5.1
|
1.0
|
CB
|
D:HIS46
|
3.5
|
4.4
|
1.0
|
CE
|
D:MET121
|
3.9
|
2.9
|
1.0
|
CB
|
D:PHE114
|
4.0
|
5.8
|
1.0
|
NE2
|
D:HIS117
|
4.0
|
4.6
|
1.0
|
C
|
D:GLY45
|
4.0
|
7.5
|
1.0
|
CD2
|
D:HIS117
|
4.1
|
7.6
|
1.0
|
N
|
D:HIS46
|
4.2
|
7.0
|
1.0
|
NE2
|
D:HIS46
|
4.2
|
7.5
|
1.0
|
CD2
|
D:HIS46
|
4.3
|
30.2
|
1.0
|
CG
|
D:MET121
|
4.4
|
12.4
|
1.0
|
C
|
D:HIS46
|
4.6
|
13.3
|
1.0
|
N
|
D:ASN47
|
4.6
|
7.3
|
1.0
|
CA
|
D:CYS112
|
4.6
|
4.3
|
1.0
|
CB
|
D:MET121
|
4.8
|
2.2
|
1.0
|
CA
|
D:HIS117
|
4.9
|
9.1
|
1.0
|
N
|
D:PHE114
|
4.9
|
17.1
|
1.0
|
CG
|
D:PHE114
|
4.9
|
14.7
|
1.0
|
|
Reference:
H.Nar,
A.Messerschmidt,
R.Huber,
M.Van De Kamp,
G.W.Canters.
X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa. J.Mol.Biol. V. 218 427 1991.
ISSN: ISSN 0022-2836
PubMed: 1901363
DOI: 10.1016/0022-2836(91)90723-J
Page generated: Tue Jul 30 23:11:52 2024
|