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Copper in PDB 2azu: X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa

Protein crystallography data

The structure of X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa, PDB code: 2azu was solved by H.Nar, A.Messerschmidt, R.Huber, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) N/A / 1.90
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 57.820, 81.060, 110.030, 90.00, 90.00, 90.00
R / Rfree (%) 17 / n/a

Copper Binding Sites:

The binding sites of Copper atom in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa (pdb code 2azu). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 4 binding sites of Copper where determined in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa, PDB code: 2azu:
Jump to Copper binding site number: 1; 2; 3; 4;

Copper binding site 1 out of 4 in 2azu

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Copper binding site 1 out of 4 in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu129

b:14.4
occ:1.00
ND1 A:HIS46 2.1 24.1 1.0
ND1 A:HIS117 2.1 9.6 1.0
SG A:CYS112 2.2 8.4 1.0
SD A:MET121 3.0 14.0 1.0
CE1 A:HIS46 3.0 8.0 1.0
CE1 A:HIS117 3.0 4.0 1.0
CG A:HIS117 3.1 9.0 1.0
CG A:HIS46 3.1 10.4 1.0
O A:GLY45 3.1 19.1 1.0
CB A:CYS112 3.2 2.0 1.0
CB A:HIS117 3.4 2.0 1.0
CA A:HIS46 3.5 6.7 1.0
CB A:HIS46 3.5 10.6 1.0
C A:GLY45 4.0 36.0 1.0
CB A:PHE114 4.0 4.2 1.0
CE A:MET121 4.1 4.4 1.0
NE2 A:HIS46 4.1 8.5 1.0
NE2 A:HIS117 4.2 7.6 1.0
N A:HIS46 4.2 10.9 1.0
CD2 A:HIS117 4.2 7.9 1.0
CD2 A:HIS46 4.3 14.7 1.0
CG A:MET121 4.5 17.9 1.0
CA A:CYS112 4.6 4.3 1.0
C A:HIS46 4.7 3.0 1.0
N A:ASN47 4.7 5.5 1.0
CA A:HIS117 4.9 16.2 1.0
CG A:PHE114 4.9 9.6 1.0
CB A:MET121 4.9 15.9 1.0
N A:PHE114 4.9 15.8 1.0

Copper binding site 2 out of 4 in 2azu

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Copper binding site 2 out of 4 in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu129

b:13.0
occ:1.00
ND1 B:HIS117 2.0 10.7 1.0
ND1 B:HIS46 2.1 18.2 1.0
SG B:CYS112 2.2 8.4 1.0
CE1 B:HIS117 3.0 14.7 1.0
O B:GLY45 3.0 9.7 1.0
CE1 B:HIS46 3.1 19.6 1.0
CG B:HIS46 3.1 21.0 1.0
CG B:HIS117 3.1 12.1 1.0
SD B:MET121 3.1 13.3 1.0
CB B:CYS112 3.2 2.0 1.0
CA B:HIS46 3.4 2.0 1.0
CB B:HIS46 3.4 12.4 1.0
CB B:HIS117 3.5 10.2 1.0
CB B:PHE114 3.8 17.7 1.0
CE B:MET121 3.9 7.2 1.0
C B:GLY45 4.0 5.8 1.0
NE2 B:HIS117 4.1 9.4 1.0
N B:HIS46 4.2 5.5 1.0
NE2 B:HIS46 4.2 16.6 1.0
CD2 B:HIS117 4.2 8.7 1.0
CD2 B:HIS46 4.2 8.7 1.0
C B:HIS46 4.6 10.6 1.0
CG B:PHE114 4.6 9.3 1.0
CA B:CYS112 4.6 4.2 1.0
CG B:MET121 4.6 14.0 1.0
N B:ASN47 4.7 2.0 1.0
N B:PHE114 4.8 10.9 1.0
CA B:PHE114 4.9 12.9 1.0
CB B:MET121 5.0 3.1 1.0
CA B:HIS117 5.0 14.3 1.0

Copper binding site 3 out of 4 in 2azu

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Copper binding site 3 out of 4 in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cu129

b:12.2
occ:1.00
ND1 C:HIS117 2.0 6.6 1.0
ND1 C:HIS46 2.1 8.7 1.0
SG C:CYS112 2.2 7.4 1.0
CE1 C:HIS117 3.0 2.9 1.0
CG C:HIS117 3.0 19.8 1.0
SD C:MET121 3.1 12.4 1.0
CE1 C:HIS46 3.1 12.5 1.0
CG C:HIS46 3.1 13.9 1.0
O C:GLY45 3.1 16.5 1.0
CB C:CYS112 3.2 5.2 1.0
CB C:HIS117 3.4 16.2 1.0
CA C:HIS46 3.4 4.5 1.0
CB C:HIS46 3.5 4.8 1.0
CE C:MET121 3.9 3.4 1.0
CB C:PHE114 3.9 5.9 1.0
C C:GLY45 4.0 12.4 1.0
NE2 C:HIS117 4.1 14.3 1.0
CD2 C:HIS117 4.2 7.8 1.0
NE2 C:HIS46 4.2 14.9 1.0
N C:HIS46 4.2 8.6 1.0
CD2 C:HIS46 4.3 14.5 1.0
C C:HIS46 4.6 12.2 1.0
N C:ASN47 4.6 5.4 1.0
CG C:MET121 4.6 8.5 1.0
CA C:CYS112 4.7 2.0 1.0
CG C:PHE114 4.8 8.4 1.0
N C:PHE114 4.8 16.9 1.0
CA C:HIS117 4.9 12.2 1.0
CA C:PHE114 5.0 7.3 1.0
CB C:MET121 5.0 8.9 1.0

Copper binding site 4 out of 4 in 2azu

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Copper binding site 4 out of 4 in the X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35*Gln and HIS35*Leu of Azurin From Pseudomonas Aeruginosa within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Cu129

b:13.1
occ:1.00
ND1 D:HIS117 2.0 5.2 1.0
ND1 D:HIS46 2.1 18.3 1.0
SG D:CYS112 2.2 8.8 1.0
SD D:MET121 2.9 13.2 1.0
CE1 D:HIS117 2.9 2.0 1.0
CG D:HIS117 2.9 6.1 1.0
CE1 D:HIS46 3.1 10.0 1.0
O D:GLY45 3.1 12.9 1.0
CG D:HIS46 3.1 13.8 1.0
CB D:CYS112 3.3 5.0 1.0
CB D:HIS117 3.3 5.4 1.0
CA D:HIS46 3.4 5.1 1.0
CB D:HIS46 3.5 4.4 1.0
CE D:MET121 3.9 2.9 1.0
CB D:PHE114 4.0 5.8 1.0
NE2 D:HIS117 4.0 4.6 1.0
C D:GLY45 4.0 7.5 1.0
CD2 D:HIS117 4.1 7.6 1.0
N D:HIS46 4.2 7.0 1.0
NE2 D:HIS46 4.2 7.5 1.0
CD2 D:HIS46 4.3 30.2 1.0
CG D:MET121 4.4 12.4 1.0
C D:HIS46 4.6 13.3 1.0
N D:ASN47 4.6 7.3 1.0
CA D:CYS112 4.6 4.3 1.0
CB D:MET121 4.8 2.2 1.0
CA D:HIS117 4.9 9.1 1.0
N D:PHE114 4.9 17.1 1.0
CG D:PHE114 4.9 14.7 1.0

Reference:

H.Nar, A.Messerschmidt, R.Huber, M.Van De Kamp, G.W.Canters. X-Ray Crystal Structure of the Two Site-Specific Mutants HIS35GLN and HIS35LEU of Azurin From Pseudomonas Aeruginosa. J.Mol.Biol. V. 218 427 1991.
ISSN: ISSN 0022-2836
PubMed: 1901363
DOI: 10.1016/0022-2836(91)90723-J
Page generated: Tue Jul 30 23:11:52 2024

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