Copper in the structure of Crystal Structure Of The Hydroxylamine-Induced Deoxy-Form of the Copper-Bound Streptomyces Castaneoglobisporus Tyrosinase in Complex With A Caddie Protein (pdb 2ahl)

The binding sites of Copper atom in the structure of Crystal Structure Of The Hydroxylamine-Induced Deoxy-Form of the Copper-Bound Streptomyces Castaneoglobisporus Tyrosinase in Complex With A Caddie Protein (pdb code 2ahl). This binding sites where shown with 5.0 Angstroms radius around Copper atom. The 2ahl structure was solved by Y.MATOBA, T.KUMAGAI, A.YAMAMOTO, H.YOSHITSU, M.SUGIYAMA, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 30.0-1.6 Space group P21212 a (A) 65.470 b (A) 97.920 c (A) 55.160 alpha (°) 90.00 beta (°) 90.00 gamma (°) 90.00 Rfactor (%) 21.5 Rfree (%) 26.3 Copper Binding Sites: Copper binding site 1 out of 3 in 2ahl Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Copper in the PDB 2ahl. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: His38, A: Ile42, A: Gly53, A: His54, A: Trp62, A: His63, A: Phe212, A: His216, B: Tyr98, A: Cu1401, B: Hoh411, conact list: Atom Atom Distance (A) Cu NE2 A:His38 2.11 Cu ND1 A:His38 4.23 Cu CD2 A:His38 2.79 Cu CE1 A:His38 3.28 Cu CG A:His38 4.04 Cu CD1 A:Ile42 4.33 Cu O A:Gly53 5.00 Cu NE2 A:His54 2.19 Cu ND1 A:His54 4.27 Cu CD2 A:His54 3.03 Cu CE1 A:His54 3.24 Cu CG A:His54 4.21 Cu CZ3 A:Trp62 4.54 Cu NE2 A:His63 2.05 Cu ND1 A:His63 4.00 Cu CD2 A:His63 3.23 Cu CE1 A:His63 2.78 Cu CG A:His63 4.24 Cu CE2 A:Phe212 4.32 Cu CZ A:Phe212 4.13 Cu NE2 A:His216 4.27 Cu CE1 A:His216 4.51 Cu OH B:Tyr98 4.52 Cu CU A:Cu1401 4.17 Cu O B:Hoh411 2.35 interactive model: Copper binding site 2 out of 3 in 2ahl Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Copper in the PDB 2ahl. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Phe59, A: His63, A: His190, A: His194, A: Phe212, A: His215, A: His216, B: Tyr98, A: Cu1400, B: Hoh411, conact list: Atom Atom Distance (A) Cu CE1 A:Phe59 4.85 Cu NE2 A:His63 4.62 Cu CD2 A:His63 5.00 Cu NE2 A:His190 2.11 Cu ND1 A:His190 4.13 Cu CD2 A:His190 3.15 Cu CE1 A:His190 3.00 Cu CG A:His190 4.23 Cu NE2 A:His194 2.09 Cu ND1 A:His194 4.13 Cu CD2 A:His194 3.11 Cu CE1 A:His194 3.00 Cu CG A:His194 4.21 Cu CE2 A:Phe212 4.13 Cu CD2 A:Phe212 4.78 Cu CZ A:Phe212 4.76 Cu NE2 A:His215 4.58 Cu CD2 A:His215 4.42 Cu NE2 A:His216 2.12 Cu ND1 A:His216 4.11 Cu CD2 A:His216 3.21 Cu CE1 A:His216 2.95 Cu CG A:His216 4.26 Cu CE2 B:Tyr98 4.03 Cu CD2 B:Tyr98 4.94 Cu CZ B:Tyr98 4.28 Cu OH B:Tyr98 3.98 Cu CU A:Cu1400 4.17 Cu O B:Hoh411 2.37 interactive model: Copper binding site 3 out of 3 in 2ahl Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 3 of Copper in the PDB 2ahl. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Ile42, A: Met43, B: His82, B: Val83, B: Met84, B: Ile92, B: His97, A: Hoh720, B: Hoh746, conact list: Atom Atom Distance (A) Cu O A:Ile42 3.50 Cu C A:Ile42 4.38 Cu O A:Met43 4.13 Cu N A:Met43 4.62 Cu CB A:Met43 4.89 Cu C A:Met43 4.40 Cu CG A:Met43 4.96 Cu CA A:Met43 3.96 Cu NE2 B:His82 2.25 Cu ND1 B:His82 4.39 Cu CD2 B:His82 3.05 Cu CE1 B:His82 3.36 Cu CG B:His82 4.28 Cu C B:Val83 4.85 Cu N B:Met84 4.66 Cu CB B:Met84 3.47 Cu CE B:Met84 3.72 Cu CG B:Met84 3.37 Cu SD B:Met84 2.50 Cu CA B:Met84 4.65 Cu CD1 B:Ile92 4.74 Cu NE2 B:His97 2.32 Cu ND1 B:His97 4.41 Cu CD2 B:His97 3.32 Cu CE1 B:His97 3.27 Cu CG B:His97 4.46 Cu O A:Hoh720 4.70 Cu O B:Hoh746 2.74 interactive model: