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Copper in PDB 1xb3: The D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin

Protein crystallography data

The structure of The D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin, PDB code: 1xb3 was solved by A.Tigerstrom, F.Schwarz, G.Karlsson, M.Okvist, C.Alvarez-Rua, D.Maeder, F.T.Robb, L.Sjolin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 59.76 / 1.50
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 81.412, 87.338, 31.366, 90.00, 90.00, 90.00
R / Rfree (%) 17 / 21.4

Copper Binding Sites:

The binding sites of Copper atom in the The D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin (pdb code 1xb3). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the The D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin, PDB code: 1xb3:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in 1xb3

Go back to Copper Binding Sites List in 1xb3
Copper binding site 1 out of 2 in the The D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of The D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu1001

b:10.8
occ:1.00
ND1 A:HIS117 2.0 9.2 1.0
ND1 A:HIS46 2.0 9.3 1.0
SG A:CYS112 2.3 10.0 1.0
O A:GLY45 2.7 12.1 1.0
CE1 A:HIS117 3.0 10.4 1.0
CE1 A:HIS46 3.0 11.7 1.0
CG A:HIS117 3.1 10.6 1.0
CG A:HIS46 3.1 10.1 1.0
CB A:CYS112 3.2 9.7 1.0
SD A:MET121 3.3 14.6 1.0
CA A:HIS46 3.3 8.8 1.0
CB A:HIS117 3.4 11.5 1.0
CB A:HIS46 3.5 9.4 1.0
C A:GLY45 3.7 10.8 1.0
CB A:PHE114 3.8 11.9 1.0
N A:HIS46 3.9 9.9 1.0
CE A:MET121 4.0 13.7 1.0
NE2 A:HIS117 4.1 11.1 1.0
NE2 A:HIS46 4.1 11.6 1.0
CD2 A:HIS117 4.2 11.0 1.0
CD2 A:HIS46 4.2 10.8 1.0
C A:HIS46 4.5 8.7 1.0
N A:ASN47 4.6 8.2 1.0
CA A:CYS112 4.6 9.9 1.0
CG A:PHE114 4.7 11.0 1.0
CG A:MET121 4.7 15.1 1.0
N A:PHE114 4.8 11.7 1.0
SD A:MET13 4.9 14.5 0.5
CA A:PHE114 4.9 12.3 1.0
CA A:HIS117 4.9 12.3 1.0

Copper binding site 2 out of 2 in 1xb3

Go back to Copper Binding Sites List in 1xb3
Copper binding site 2 out of 2 in the The D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of The D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu1002

b:10.3
occ:1.00
ND1 B:HIS117 2.0 9.3 1.0
ND1 B:HIS46 2.1 8.7 1.0
SG B:CYS112 2.2 8.7 1.0
O B:GLY45 2.8 10.1 1.0
CE1 B:HIS117 3.0 10.7 1.0
CE1 B:HIS46 3.0 8.9 1.0
CG B:HIS117 3.1 9.2 1.0
CG B:HIS46 3.1 9.3 1.0
SD B:MET121 3.3 11.3 1.0
CB B:CYS112 3.3 9.7 1.0
CA B:HIS46 3.3 8.3 1.0
CB B:HIS117 3.4 10.3 1.0
CB B:HIS46 3.5 8.5 1.0
C B:GLY45 3.7 8.8 1.0
CB B:PHE114 3.8 9.5 1.0
CE B:MET121 3.9 11.8 1.0
N B:HIS46 4.0 8.4 1.0
NE2 B:HIS117 4.1 10.2 1.0
NE2 B:HIS46 4.1 9.6 1.0
CD2 B:HIS117 4.2 9.1 1.0
CD2 B:HIS46 4.2 10.1 1.0
C B:HIS46 4.5 7.7 1.0
N B:ASN47 4.6 7.9 1.0
CG B:PHE114 4.7 8.8 1.0
CA B:CYS112 4.7 9.6 1.0
CG B:MET121 4.7 12.0 1.0
N B:PHE114 4.7 9.4 1.0
CA B:PHE114 4.9 8.9 1.0
CB B:MET121 4.9 11.6 1.0
O B:MET44 4.9 12.8 1.0
CA B:HIS117 5.0 11.0 1.0

Reference:

A.Tigerstrom, F.Schwarz, G.Karlsson, M.Okvist, C.Alvarez-Rua, D.Maeder, F.T.Robb, L.Sjolin. Effects of A Novel Disulfide Bond and Engineered Electrostatic Interactions on the Thermostability of Azurin Biochemistry V. 43 12563 2004.
ISSN: ISSN 0006-2960
PubMed: 15449946
DOI: 10.1021/BI048926X
Page generated: Sun Dec 13 11:02:59 2020

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