Copper in PDB 1xb3: The D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin

The structure of The D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin, PDB code: 1xb3 was solved by A.Tigerstrom, F.Schwarz, G.Karlsson, M.Okvist, C.Alvarez-Rua, D.Maeder, F.T.Robb, L.Sjolin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	59.76 / 1.50
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	81.412, 87.338, 31.366, 90.00, 90.00, 90.00
R / Rfree (%)	17 / 21.4

The binding sites of Copper atom in the The D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin (pdb code 1xb3). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the The D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin, PDB code: 1xb3:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the The D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of The D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu1001 b:10.8 occ:1.00 ND1 A:HIS117 2.0 9.2 1.0 ND1 A:HIS46 2.0 9.3 1.0 SG A:CYS112 2.3 10.0 1.0 O A:GLY45 2.7 12.1 1.0 CE1 A:HIS117 3.0 10.4 1.0 CE1 A:HIS46 3.0 11.7 1.0 CG A:HIS117 3.1 10.6 1.0 CG A:HIS46 3.1 10.1 1.0 CB A:CYS112 3.2 9.7 1.0 SD A:MET121 3.3 14.6 1.0 CA A:HIS46 3.3 8.8 1.0 CB A:HIS117 3.4 11.5 1.0 CB A:HIS46 3.5 9.4 1.0 C A:GLY45 3.7 10.8 1.0 CB A:PHE114 3.8 11.9 1.0 N A:HIS46 3.9 9.9 1.0 CE A:MET121 4.0 13.7 1.0 NE2 A:HIS117 4.1 11.1 1.0 NE2 A:HIS46 4.1 11.6 1.0 CD2 A:HIS117 4.2 11.0 1.0 CD2 A:HIS46 4.2 10.8 1.0 C A:HIS46 4.5 8.7 1.0 N A:ASN47 4.6 8.2 1.0 CA A:CYS112 4.6 9.9 1.0 CG A:PHE114 4.7 11.0 1.0 CG A:MET121 4.7 15.1 1.0 N A:PHE114 4.8 11.7 1.0 SD A:MET13 4.9 14.5 0.5 CA A:PHE114 4.9 12.3 1.0 CA A:HIS117 4.9 12.3 1.0

Copper binding site 2 out of 2 in the The D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of The D62C/K74C Double Mutant of Pseudomonas Aeruginosa Azurin within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu1002 b:10.3 occ:1.00 ND1 B:HIS117 2.0 9.3 1.0 ND1 B:HIS46 2.1 8.7 1.0 SG B:CYS112 2.2 8.7 1.0 O B:GLY45 2.8 10.1 1.0 CE1 B:HIS117 3.0 10.7 1.0 CE1 B:HIS46 3.0 8.9 1.0 CG B:HIS117 3.1 9.2 1.0 CG B:HIS46 3.1 9.3 1.0 SD B:MET121 3.3 11.3 1.0 CB B:CYS112 3.3 9.7 1.0 CA B:HIS46 3.3 8.3 1.0 CB B:HIS117 3.4 10.3 1.0 CB B:HIS46 3.5 8.5 1.0 C B:GLY45 3.7 8.8 1.0 CB B:PHE114 3.8 9.5 1.0 CE B:MET121 3.9 11.8 1.0 N B:HIS46 4.0 8.4 1.0 NE2 B:HIS117 4.1 10.2 1.0 NE2 B:HIS46 4.1 9.6 1.0 CD2 B:HIS117 4.2 9.1 1.0 CD2 B:HIS46 4.2 10.1 1.0 C B:HIS46 4.5 7.7 1.0 N B:ASN47 4.6 7.9 1.0 CG B:PHE114 4.7 8.8 1.0 CA B:CYS112 4.7 9.6 1.0 CG B:MET121 4.7 12.0 1.0 N B:PHE114 4.7 9.4 1.0 CA B:PHE114 4.9 8.9 1.0 CB B:MET121 4.9 11.6 1.0 O B:MET44 4.9 12.8 1.0 CA B:HIS117 5.0 11.0 1.0

A.Tigerstrom, F.Schwarz, G.Karlsson, M.Okvist, C.Alvarez-Rua, D.Maeder, F.T.Robb, L.Sjolin. Effects of A Novel Disulfide Bond and Engineered Electrostatic Interactions on the Thermostability of Azurin Biochemistry V. 43 12563 2004.
ISSN: ISSN 0006-2960
PubMed: 15449946
DOI: 10.1021/BI048926X