Copper in PDB 1wx4: Crystal Structure of the Oxy-Form of the Copper-Bound Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein Prepared By the Addition of Dithiothreitol

Enzymatic activity of Crystal Structure of the Oxy-Form of the Copper-Bound Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein Prepared By the Addition of Dithiothreitol

All present enzymatic activity of Crystal Structure of the Oxy-Form of the Copper-Bound Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein Prepared By the Addition of Dithiothreitol:
1.14.18.1;

Protein crystallography data

The structure of Crystal Structure of the Oxy-Form of the Copper-Bound Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein Prepared By the Addition of Dithiothreitol, PDB code: 1wx4 was solved by Y.Matoba, T.Kumagai, A.Yamamoto, H.Yoshitsu, M.Sugiyama, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	30.00 / 1.50
*Space group*	P 2₁ 2₁ 2
*Cell size a, b, c (Å), α, β, γ (°)*	65.360, 98.230, 55.170, 90.00, 90.00, 90.00
*R / R_free (%)*	18.8 / 21.6

Copper Binding Sites:

The binding sites of Copper atom in the Crystal Structure of the Oxy-Form of the Copper-Bound Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein Prepared By the Addition of Dithiothreitol (pdb code 1wx4). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 4 binding sites of Copper where determined in the Crystal Structure of the Oxy-Form of the Copper-Bound Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein Prepared By the Addition of Dithiothreitol, PDB code: 1wx4:
Jump to Copper binding site number: 1; 2; 3; 4;

Copper binding site 1 out of 4 in 1wx4

Go back to

Copper Binding Sites List in 1wx4

Copper binding site 1 out of 4 in the Crystal Structure of the Oxy-Form of the Copper-Bound Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein Prepared By the Addition of Dithiothreitol

Mono view

Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure of the Oxy-Form of the Copper-Bound Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein Prepared By the Addition of Dithiothreitol within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Cu400 b:24.7 occ:1.00	O2	A:PER409	2.0	20.3	1.0
	O1	A:PER409	2.0	22.2	1.0
	NE2	A:HIS38	2.1	15.7	1.0
	NE2	A:HIS54	2.2	19.7	1.0
	NE2	A:HIS63	2.4	10.3	1.0
	CD2	A:HIS54	2.8	16.7	1.0
	CE1	A:HIS63	3.0	9.5	1.0
	CE1	A:HIS38	3.1	14.7	1.0
	CD2	A:HIS38	3.1	13.8	1.0
	CE1	A:HIS54	3.3	18.1	1.0
	CU	A:CU401	3.6	15.3	1.0
	CD2	A:HIS63	3.6	8.9	1.0
	OH	B:TYR98	4.0	16.5	1.0
	CG	A:HIS54	4.1	16.6	1.0
	NE2	A:HIS216	4.1	10.3	1.0
	ND1	A:HIS38	4.2	13.8	1.0
	CG	A:HIS38	4.2	13.7	1.0
	ND1	A:HIS63	4.2	9.6	1.0
	ND1	A:HIS54	4.3	17.5	1.0
	CE2	A:PHE212	4.4	8.8	1.0
	CE1	A:HIS216	4.5	8.1	1.0
	CZ	A:PHE212	4.5	7.7	1.0
	CD1	A:ILE42	4.5	18.4	1.0
	CG	A:HIS63	4.6	9.4	1.0
	CE2	B:TYR98	4.8	14.4	1.0
	CZ	B:TYR98	4.9	14.7	1.0
	NE2	A:HIS190	4.9	8.0	1.0
	CE1	A:PHE59	4.9	7.5	1.0

Copper binding site 2 out of 4 in 1wx4

Go back to

Copper Binding Sites List in 1wx4

Copper binding site 2 out of 4 in the Crystal Structure of the Oxy-Form of the Copper-Bound Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein Prepared By the Addition of Dithiothreitol

Mono view

Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure of the Oxy-Form of the Copper-Bound Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein Prepared By the Addition of Dithiothreitol within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Cu401 b:15.3 occ:1.00	O2	A:PER409	2.0	20.3	1.0
	O1	A:PER409	2.0	22.2	1.0
	NE2	A:HIS190	2.1	8.0	1.0
	NE2	A:HIS194	2.1	11.3	1.0
	NE2	A:HIS216	2.2	10.3	1.0
	CE1	A:HIS190	3.0	8.6	1.0
	CE1	A:HIS216	3.0	8.1	1.0
	CE1	A:HIS194	3.0	10.3	1.0
	CD2	A:HIS194	3.1	10.4	1.0
	CD2	A:HIS190	3.1	7.8	1.0
	CD2	A:HIS216	3.3	8.7	1.0
	CU	A:CU400	3.6	24.7	1.0
	CE2	B:TYR98	3.9	14.4	1.0
	OH	B:TYR98	4.0	16.5	1.0
	ND1	A:HIS190	4.1	8.4	1.0
	ND1	A:HIS194	4.1	11.0	1.0
	CG	A:HIS194	4.2	9.3	1.0
	ND1	A:HIS216	4.2	7.6	1.0
	CG	A:HIS190	4.2	9.0	1.0
	CZ	B:TYR98	4.2	14.7	1.0
	CG	A:HIS216	4.3	8.3	1.0
	CE2	A:PHE212	4.3	8.8	1.0
	NE2	A:HIS63	4.4	10.3	1.0
	CD2	A:HIS215	4.6	9.5	1.0
	CE1	A:PHE59	4.8	7.5	1.0
	NE2	A:HIS215	4.8	9.1	1.0
	CD2	B:TYR98	4.8	14.3	1.0
	NE2	A:HIS38	4.8	15.7	1.0
	CD2	A:HIS63	4.9	8.9	1.0
	CD2	A:PHE212	5.0	7.8	1.0
	CZ	A:PHE212	5.0	7.7	1.0

Copper binding site 3 out of 4 in 1wx4

Go back to

Copper Binding Sites List in 1wx4

Copper binding site 3 out of 4 in the Crystal Structure of the Oxy-Form of the Copper-Bound Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein Prepared By the Addition of Dithiothreitol

Mono view

Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Crystal Structure of the Oxy-Form of the Copper-Bound Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein Prepared By the Addition of Dithiothreitol within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Cu403 b:56.9 occ:0.50	O	A:HOH707	2.8	31.5	0.5
	O	A:HOH725	4.9	33.5	1.0
	CG	A:PRO231	4.9	20.5	1.0

Copper binding site 4 out of 4 in 1wx4

Go back to

Copper Binding Sites List in 1wx4

Copper binding site 4 out of 4 in the Crystal Structure of the Oxy-Form of the Copper-Bound Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein Prepared By the Addition of Dithiothreitol

Mono view

Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Crystal Structure of the Oxy-Form of the Copper-Bound Streptomyces Castaneoglobisporus Tyrosinase Complexed with A Caddie Protein Prepared By the Addition of Dithiothreitol within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
B:Cu402 b:48.0 occ:1.00	NE2	B:HIS82	2.1	32.6	1.0
	CD2	B:HIS82	2.9	26.7	1.0
	CE1	B:HIS82	3.2	26.8	1.0
	O	A:HOH751	3.4	28.6	1.0
	CG	B:HIS82	4.1	23.7	1.0
	ND1	B:HIS82	4.2	25.8	1.0
	O	A:MET43	4.3	16.6	1.0
	O	B:HOH596	5.0	20.9	1.0

Reference:

Y.Matoba, T.Kumagai, A.Yamamoto, H.Yoshitsu, M.Sugiyama. Crystallographic Evidence That the Dinuclear Copper Center of Tyrosinase Is Flexible During Catalysis J.Biol.Chem. V. 281 8981 2006.
ISSN: ISSN 0021-9258
PubMed: 16436386
DOI: 10.1074/JBC.M509785200

Page generated: Tue Jul 30 23:00:09 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW

	© Copyright 2008-2020 by atomistry.com
Home \| Site Map \| Copyright \| Contact us \| Privacy