Copper in the structure of Crystal Structure Of The Oxy-Form Of The Copper-Bound Streptomyces Castaneoglobisporus Tyrosinase Complexed With A Caddie Protein Prepared By the Addition of Hydrogenperoxide (pdb 1wx2)

The binding sites of Copper atom in the structure of Crystal Structure Of The Oxy-Form Of The Copper-Bound Streptomyces Castaneoglobisporus Tyrosinase Complexed With A Caddie Protein Prepared By the Addition of Hydrogenperoxide (pdb code 1wx2). This binding sites where shown with 5.0 Angstroms radius around Copper atom. The 1wx2 structure was solved by Y.MATOBA, T.KUMAGAI, A.YAMAMOTO, H.YOSHITSU, M.SUGIYAMA, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 30.0-1.8 Space group P21212 a (A) 65.160 b (A) 96.550 c (A) 54.720 alpha (°) 90.00 beta (°) 90.00 gamma (°) 90.00 Rfactor (%) 21 Rfree (%) 24.9 Copper Binding Sites: Copper binding site 1 out of 5 in 1wx2 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Copper in the PDB 1wx2. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: His38, A: Ile42, A: His54, A: Phe59, A: His63, A: His190, A: Phe212, A: His216, B: Tyr98, A: Cu401, A: Per404, conact list: Atom Atom Distance (A) Cu NE2 A:His38 2.14 Cu ND1 A:His38 4.26 Cu CD2 A:His38 3.00 Cu CE1 A:His38 3.21 Cu CG A:His38 4.20 Cu CD1 A:Ile42 4.11 Cu NE2 A:His54 2.20 Cu ND1 A:His54 4.32 Cu CD2 A:His54 2.96 Cu CE1 A:His54 3.32 Cu CG A:His54 4.19 Cu CE1 A:Phe59 4.90 Cu NE2 A:His63 2.21 Cu ND1 A:His63 4.12 Cu CD2 A:His63 3.37 Cu CE1 A:His63 2.91 Cu CG A:His63 4.37 Cu NE2 A:His190 4.90 Cu CE2 A:Phe212 4.40 Cu CZ A:Phe212 4.56 Cu NE2 A:His216 4.02 Cu CD2 A:His216 4.96 Cu CE1 A:His216 4.29 Cu OH B:Tyr98 4.37 Cu CU A:Cu401 3.48 Cu O1 A:Per404 1.96 Cu O2 A:Per404 1.89 interactive model: Copper binding site 2 out of 5 in 1wx2 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Copper in the PDB 1wx2. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: His38, A: Phe59, A: His63, A: His190, A: His194, A: Phe212, A: His215, A: His216, B: Tyr98, A: Cu400, A: Per404, conact list: Atom Atom Distance (A) Cu NE2 A:His38 4.83 Cu CE1 A:Phe59 4.75 Cu NE2 A:His63 4.45 Cu CD2 A:His63 4.86 Cu NE2 A:His190 2.09 Cu ND1 A:His190 4.10 Cu CD2 A:His190 3.15 Cu CE1 A:His190 2.95 Cu CG A:His190 4.22 Cu NE2 A:His194 2.07 Cu ND1 A:His194 4.09 Cu CD2 A:His194 3.09 Cu CE1 A:His194 2.98 Cu CG A:His194 4.18 Cu CE2 A:Phe212 4.45 Cu NE2 A:His215 4.69 Cu CD2 A:His215 4.69 Cu NE2 A:His216 2.15 Cu ND1 A:His216 4.16 Cu CD2 A:His216 3.26 Cu CE1 A:His216 2.98 Cu CG A:His216 4.32 Cu CE2 B:Tyr98 3.99 Cu CD2 B:Tyr98 4.81 Cu CZ B:Tyr98 4.44 Cu OH B:Tyr98 4.25 Cu CU A:Cu400 3.48 Cu O1 A:Per404 2.12 Cu O2 A:Per404 1.92 interactive model: Copper binding site 3 out of 5 in 1wx2 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 3 of Copper in the PDB 1wx2. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Glu175, A: His180, A: Arg185, A: Gly186, A: Hoh453, A: Hoh567, conact list: Atom Atom Distance (A) Cu OE1 A:Glu175 3.87 Cu OE2 A:Glu175 3.32 Cu CD A:Glu175 4.00 Cu NE2 A:His180 2.17 Cu ND1 A:His180 4.26 Cu CD2 A:His180 3.10 Cu CE1 A:His180 3.18 Cu CG A:His180 4.26 Cu C A:Arg185 4.94 Cu N A:Gly186 4.56 Cu CA A:Gly186 4.29 Cu O A:Hoh453 4.91 Cu O A:Hoh567 4.24 interactive model: Copper binding site 4 out of 5 in 1wx2 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 4 of Copper in the PDB 1wx2. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Met43, B: His82, B: Hoh562, conact list: Atom Atom Distance (A) Cu O A:Met43 4.00 Cu C A:Met43 4.95 Cu NE2 B:His82 2.13 Cu NE2 B:His82 4.21 Cu ND1 B:His82 4.25 Cu ND1 B:His82 3.15 Cu CD2 B:His82 2.93 Cu CE1 B:His82 3.23 Cu CE1 B:His82 2.92 Cu CG B:His82 4.15 Cu CG B:His82 4.53 Cu O B:Hoh562 4.78 interactive model: Copper binding site 5 out of 5 in 1wx2 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 5 of Copper in the PDB 1wx2. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Ile42, A: Met43, B: His82, B: Val83, B: Met84, B: Ile92, B: His97, A: Hoh617, B: Hoh644, conact list: Atom Atom Distance (A) Cu O A:Ile42 3.34 Cu C A:Ile42 4.26 Cu O A:Met43 4.05 Cu N A:Met43 4.54 Cu CB A:Met43 4.81 Cu C A:Met43 4.34 Cu CG A:Met43 4.77 Cu CA A:Met43 3.88 Cu NE2 B:His82 4.66 Cu NE2 B:His82 2.44 Cu ND1 B:His82 3.33 Cu ND1 B:His82 4.57 Cu CD2 B:His82 2.96 Cu CE1 B:His82 3.34 Cu CE1 B:His82 3.67 Cu CG B:His82 4.70 Cu CG B:His82 4.26 Cu O B:Val83 4.79 Cu C B:Val83 4.71 Cu N B:Met84 4.60 Cu CB B:Met84 3.38 Cu CE B:Met84 4.50 Cu CG B:Met84 3.03 Cu SD B:Met84 2.76 Cu CA B:Met84 4.57 Cu CD1 B:Ile92 4.87 Cu NE2 B:His97 3.12 Cu NE2 B:His97 2.48 Cu ND1 B:His97 3.36 Cu ND1 B:His97 4.62 Cu CD2 B:His97 4.36 Cu CD2 B:His97 3.26 Cu CE1 B:His97 2.30 Cu CE1 B:His97 3.59 Cu CG B:His97 4.48 Cu CG B:His97 4.50 Cu O A:Hoh617 4.75 Cu O B:Hoh644 2.26 interactive model: