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Copper in PDB 1uri: Azurin Mutant with Met 121 Replaced By Gln

Protein crystallography data

The structure of Azurin Mutant with Met 121 Replaced By Gln, PDB code: 1uri was solved by A.Romero, H.Nar, R.Huber, A.Messerschmidt, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 8.00 / 1.94
Space group C 2 2 21
Cell size a, b, c (Å), α, β, γ (°) 75.480, 74.340, 98.200, 90.00, 90.00, 90.00
R / Rfree (%) 17.3 / n/a

Copper Binding Sites:

The binding sites of Copper atom in the Azurin Mutant with Met 121 Replaced By Gln (pdb code 1uri). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Azurin Mutant with Met 121 Replaced By Gln, PDB code: 1uri:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in 1uri

Go back to Copper Binding Sites List in 1uri
Copper binding site 1 out of 2 in the Azurin Mutant with Met 121 Replaced By Gln


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Azurin Mutant with Met 121 Replaced By Gln within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu200

b:15.7
occ:1.00
ND1 A:HIS46 1.9 7.0 1.0
ND1 A:HIS117 2.1 20.0 1.0
SG A:CYS112 2.1 8.0 1.0
OE1 A:GLN121 2.3 7.7 1.0
CG A:HIS46 2.9 6.7 1.0
CE1 A:HIS46 2.9 8.7 1.0
CG A:HIS117 3.0 5.5 1.0
CE1 A:HIS117 3.1 5.0 1.0
CB A:CYS112 3.1 6.7 1.0
CD A:GLN121 3.2 8.7 1.0
CB A:HIS46 3.3 18.1 1.0
CB A:HIS117 3.4 14.1 1.0
CA A:HIS46 3.4 14.0 1.0
O A:GLY45 3.5 15.6 1.0
NE2 A:GLN121 3.7 16.7 1.0
NE2 A:HIS46 4.0 7.9 1.0
CD2 A:HIS46 4.1 15.9 1.0
NE2 A:HIS117 4.2 9.6 1.0
CB A:PHE114 4.2 7.3 1.0
CD2 A:HIS117 4.2 8.4 1.0
C A:GLY45 4.3 11.9 1.0
N A:HIS46 4.4 12.7 1.0
N A:ASN47 4.4 9.9 1.0
C A:HIS46 4.5 11.3 1.0
CG A:GLN121 4.5 9.6 1.0
CA A:CYS112 4.5 16.9 1.0
CB A:GLN121 4.8 8.1 1.0
CA A:HIS117 4.9 16.7 1.0
C A:CYS112 4.9 19.8 1.0
CG A:MET13 5.0 39.2 1.0

Copper binding site 2 out of 2 in 1uri

Go back to Copper Binding Sites List in 1uri
Copper binding site 2 out of 2 in the Azurin Mutant with Met 121 Replaced By Gln


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Azurin Mutant with Met 121 Replaced By Gln within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu201

b:13.8
occ:1.00
ND1 B:HIS46 2.0 12.7 1.0
ND1 B:HIS117 2.0 15.3 1.0
SG B:CYS112 2.1 11.4 1.0
OE1 B:GLN121 2.3 13.4 1.0
CE1 B:HIS46 2.9 21.1 1.0
CE1 B:HIS117 3.0 9.2 1.0
CG B:HIS46 3.0 23.7 1.0
CG B:HIS117 3.1 13.2 1.0
CB B:CYS112 3.2 9.8 1.0
CD B:GLN121 3.2 15.0 1.0
O B:GLY45 3.3 11.4 1.0
CB B:HIS117 3.4 11.2 1.0
CA B:HIS46 3.5 6.3 1.0
CB B:HIS46 3.5 4.8 1.0
NE2 B:GLN121 3.6 13.2 1.0
NE2 B:HIS46 4.1 9.1 1.0
NE2 B:HIS117 4.1 8.9 1.0
CB B:PHE114 4.1 9.8 1.0
CD2 B:HIS46 4.2 13.4 1.0
C B:GLY45 4.2 15.4 1.0
CD2 B:HIS117 4.2 14.5 1.0
N B:HIS46 4.3 10.8 1.0
CG B:GLN121 4.6 12.3 1.0
N B:ASN47 4.6 5.7 1.0
CA B:CYS112 4.6 7.5 1.0
C B:HIS46 4.6 16.0 1.0
CB B:GLN121 4.8 18.8 1.0
CG B:PHE114 4.9 20.6 1.0
CA B:HIS117 5.0 5.5 1.0

Reference:

A.Romero, C.W.Hoitink, H.Nar, R.Huber, A.Messerschmidt, G.W.Canters. X-Ray Analysis and Spectroscopic Characterization of M121Q Azurin. A Copper Site Model For Stellacyanin. J.Mol.Biol. V. 229 1007 1993.
ISSN: ISSN 0022-2836
PubMed: 8383207
DOI: 10.1006/JMBI.1993.1101
Page generated: Thu Sep 3 16:21:36 2020
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