Copper in the structure of Site-Directed Mutagenesis Of HIS592 Involved In Binding of Copper Ion in Arthrobacter Globiformis Amine Oxidase (pdb 1ui8)

The binding sites of Copper atom in the structure of Site-Directed Mutagenesis Of HIS592 Involved In Binding of Copper Ion in Arthrobacter Globiformis Amine Oxidase (pdb code 1ui8). This binding sites where shown with 5.0 Angstroms radius around Copper atom. The 1ui8 structure was solved by H.MATSUNAMI, T.OKAJIMA, S.HIROTA, H.YAMAGUCHI, H.HORI, S.KURODA, K.TANIZAWA, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 10.0-1.8 Space group I121 a (A) 157.762 b (A) 63.210 c (A) 184.244 alpha (°) 90.00 beta (°) 111.64 gamma (°) 90.00 Rfactor (%) 21.7 Rfree (%) 25.8 Copper Binding Sites: Copper binding site 1 out of 2 in 1ui8 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Copper in the PDB 1ui8. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Tpq382, A: His431, A: His433, A: Ala592, A: Met602, A: Hoh1185, conact list: Atom Atom Distance (A) Cu O5 A:Tpq382 2.59 Cu C6 A:Tpq382 4.42 Cu C5 A:Tpq382 3.10 Cu C4 A:Tpq382 2.83 Cu C3 A:Tpq382 4.05 Cu O4 A:Tpq382 1.93 Cu NE2 A:His431 2.21 Cu ND1 A:His431 4.30 Cu CD2 A:His431 3.20 Cu CE1 A:His431 3.18 Cu CG A:His431 4.34 Cu NE2 A:His433 2.32 Cu ND1 A:His433 4.45 Cu CD2 A:His433 3.09 Cu CE1 A:His433 3.43 Cu CG A:His433 4.32 Cu CB A:Ala592 4.30 Cu CE A:Met602 4.58 Cu SD A:Met602 4.70 Cu O A:Hoh1185 4.09 interactive model: Copper binding site 2 out of 2 in 1ui8 Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Copper in the PDB 1ui8. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: B: Tpq382, B: His431, B: His433, B: Ala592, B: Met602, B: Hoh1105, B: Hoh1435, conact list: Atom Atom Distance (A) Cu O5 B:Tpq382 2.43 Cu C6 B:Tpq382 4.39 Cu C5 B:Tpq382 3.04 Cu C4 B:Tpq382 2.83 Cu C3 B:Tpq382 4.09 Cu O4 B:Tpq382 1.99 Cu NE2 B:His431 2.20 Cu ND1 B:His431 4.29 Cu CD2 B:His431 3.17 Cu CE1 B:His431 3.19 Cu CG B:His431 4.32 Cu NE2 B:His433 2.41 Cu ND1 B:His433 4.53 Cu CD2 B:His433 3.11 Cu CE1 B:His433 3.53 Cu CG B:His433 4.36 Cu CB B:Ala592 4.28 Cu CE B:Met602 4.46 Cu SD B:Met602 4.55 Cu O B:Hoh1105 4.09 Cu O B:Hoh1435 4.54 interactive model: