Copper in PDB 1teg: Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond

The structure of Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond, PDB code: 1teg was solved by M.Okvist, F.Jacobson, H.Jansson, O.Hansson, L.Sjolin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.20 / 1.96
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	52.196, 52.196, 126.928, 90.00, 90.00, 120.00
R / Rfree (%)	28.7 / 30.4

The binding sites of Copper atom in the Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond (pdb code 1teg). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond, PDB code: 1teg:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu101 b:17.0 occ:1.00 ND1 A:HIS87 2.0 12.0 1.0 SG A:CYS84 2.1 10.9 1.0 ND1 A:HIS37 2.2 13.9 1.0 SD A:MET92 2.9 9.6 1.0 CE1 A:HIS87 2.9 12.8 1.0 CG A:HIS87 3.0 12.2 1.0 CE1 A:HIS37 3.1 14.4 1.0 CB A:CYS84 3.2 11.9 1.0 CG A:HIS37 3.3 12.0 1.0 CB A:HIS87 3.4 12.4 1.0 O A:PRO36 3.6 13.8 1.0 CE A:MET92 3.6 7.8 1.0 CA A:HIS37 3.6 14.9 1.0 CB A:HIS37 3.7 14.1 1.0 NE2 A:HIS87 4.0 12.8 1.0 CD2 A:HIS87 4.1 11.3 1.0 CG A:PRO86 4.2 15.8 1.0 N A:HIS87 4.3 13.8 1.0 NE2 A:HIS37 4.3 12.7 1.0 CG A:MET92 4.3 11.5 1.0 CD2 A:HIS37 4.4 11.9 1.0 C A:PRO36 4.4 16.2 1.0 N A:HIS37 4.5 15.1 1.0 CA A:HIS87 4.5 13.0 1.0 CA A:CYS84 4.6 12.9 1.0 CB A:MET92 4.7 13.3 1.0 N A:ASN38 4.7 13.9 1.0 C A:HIS37 4.8 15.3 1.0 O A:GLY34 4.8 14.7 1.0

Copper binding site 2 out of 2 in the Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu102 b:15.1 occ:1.00 ND1 B:HIS37 2.0 8.7 1.0 SG B:CYS84 2.2 15.4 1.0 ND1 B:HIS87 2.2 14.9 1.0 SD B:MET92 2.8 14.9 1.0 CE1 B:HIS37 2.8 9.1 1.0 CG B:HIS37 3.0 10.6 1.0 CE1 B:HIS87 3.1 14.3 1.0 CG B:HIS87 3.1 14.5 1.0 CB B:CYS84 3.2 15.3 1.0 CB B:HIS37 3.5 10.9 1.0 CB B:HIS87 3.5 15.1 1.0 CE B:MET92 3.5 13.9 1.0 CA B:HIS37 3.8 12.2 1.0 NE2 B:HIS37 4.0 10.4 1.0 O B:PRO36 4.1 14.0 1.0 CG B:MET92 4.1 15.7 1.0 CD2 B:HIS37 4.1 9.5 1.0 NE2 B:HIS87 4.1 13.6 1.0 CD2 B:HIS87 4.2 14.4 1.0 CG B:PRO86 4.4 15.6 1.0 N B:HIS87 4.6 15.9 1.0 CA B:CYS84 4.6 15.4 1.0 CB B:MET92 4.7 16.7 1.0 CA B:HIS87 4.7 15.6 1.0 N B:HIS37 4.7 13.4 1.0 C B:PRO36 4.8 14.5 1.0 N B:ASN38 4.8 13.3 1.0 C B:HIS37 4.9 12.8 1.0

M.Okvist, F.Jacobson, H.Jansson, O.Hansson, L.Sjolin. Novel Disulfide Bonds Effect the Thermostability of Plastocyanin. Crystal Structures of the Triple Plastocyanin Mutant G8D/K30C/T69C and the Double Plastocyanin Mutant K30C/T69C From Spinach at 1.90 A and 1.96 A Resolution, Respectively. To Be Published.