The binding sites of Copper atom in the structure of Crystal Structure Of The Spinach Plastocyanin Mutants G8D/K30C/T69C And K30C/T69C- A Study Of The Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond (pdb code 1teg). This binding sites where shown with 5.0 Angstroms radius around Copper atom. The 1teg structure was solved by M.OKVIST, F.JACOBSON, H.JANSSON, O.HANSSON, L.SJOLIN, with X-Ray Crystallography technique, brief refinement statistics is given in the table below:
Resolution (A) | 45.2-2.0 | Space group | P3221 | a (A) | 52.196 | b (A) | 52.196 | c (A) | 126.928 | alpha (°) | 90.00 | beta (°) | 90.00 | gamma (°) | 120.00 | Rfactor (%) | 28.7 | Rfree (%) | 30.4 |
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Copper binding site 1 out of 2 in 1teg
|  Click to enlarge |  Click to enlarge | Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Copper in the PDB 1teg. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Gly34, A: Pro36, A: His37, A: Asn38, A: Cys84, A: Pro86, A: His87, A: Met92, | conact list:
Atom | Atom | Distance (A) | Cu | O A:Gly34 | 4.79 | Cu | O A:Pro36 | 3.59 | Cu | C A:Pro36 | 4.42 | Cu | NE2 A:His37 | 4.26 | Cu | N A:His37 | 4.47 | Cu | CB A:His37 | 3.72 | Cu | ND1 A:His37 | 2.23 | Cu | CD2 A:His37 | 4.39 | Cu | C A:His37 | 4.76 | Cu | CE1 A:His37 | 3.07 | Cu | CG A:His37 | 3.33 | Cu | CA A:His37 | 3.62 | Cu | N A:Asn38 | 4.74 | Cu | CB A:Cys84 | 3.21 | Cu | SG A:Cys84 | 2.15 | Cu | CA A:Cys84 | 4.64 | Cu | CG A:Pro86 | 4.23 | Cu | NE2 A:His87 | 4.03 | Cu | N A:His87 | 4.25 | Cu | CB A:His87 | 3.42 | Cu | ND1 A:His87 | 2.03 | Cu | CD2 A:His87 | 4.10 | Cu | CE1 A:His87 | 2.92 | Cu | CG A:His87 | 3.04 | Cu | CA A:His87 | 4.48 | Cu | CB A:Met92 | 4.70 | Cu | CE A:Met92 | 3.62 | Cu | CG A:Met92 | 4.27 | Cu | SD A:Met92 | 2.90 |
| interactive model:
| Copper binding site 2 out of 2 in 1teg
|  Click to enlarge |  Click to enlarge | Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Copper in the PDB 1teg. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: B: Pro36, B: His37, B: Asn38, B: Cys84, B: Pro86, B: His87, B: Met92, | conact list:
Atom | Atom | Distance (A) | Cu | O B:Pro36 | 4.08 | Cu | C B:Pro36 | 4.81 | Cu | NE2 B:His37 | 4.00 | Cu | N B:His37 | 4.72 | Cu | CB B:His37 | 3.48 | Cu | ND1 B:His37 | 1.95 | Cu | CD2 B:His37 | 4.11 | Cu | C B:His37 | 4.87 | Cu | CE1 B:His37 | 2.85 | Cu | CG B:His37 | 3.04 | Cu | CA B:His37 | 3.76 | Cu | N B:Asn38 | 4.81 | Cu | CB B:Cys84 | 3.20 | Cu | SG B:Cys84 | 2.20 | Cu | CA B:Cys84 | 4.63 | Cu | CG B:Pro86 | 4.42 | Cu | NE2 B:His87 | 4.13 | Cu | N B:His87 | 4.56 | Cu | CB B:His87 | 3.51 | Cu | ND1 B:His87 | 2.21 | Cu | CD2 B:His87 | 4.18 | Cu | CE1 B:His87 | 3.07 | Cu | CG B:His87 | 3.13 | Cu | CA B:His87 | 4.68 | Cu | CB B:Met92 | 4.66 | Cu | CE B:Met92 | 3.52 | Cu | CG B:Met92 | 4.11 | Cu | SD B:Met92 | 2.79 |
| interactive model:
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