Copper in PDB 1tef: Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond

The structure of Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond, PDB code: 1tef was solved by M.Okvist, F.Jacobson, H.Jansson, O.Hansson, L.Sjolin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.20 / 1.90
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	52.320, 52.320, 127.360, 90.00, 90.00, 120.00
R / Rfree (%)	15.7 / 20.4

The structure of Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

The binding sites of Copper atom in the Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond (pdb code 1tef). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond, PDB code: 1tef:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu401 b:12.4 occ:1.00 ND1 A:HIS37 2.1 8.7 1.0 ND1 A:HIS87 2.1 11.7 1.0 SG A:CYS84 2.2 11.0 1.0 SD A:MET92 2.7 9.4 1.0 CE1 A:HIS37 3.0 9.5 1.0 CE1 A:HIS87 3.1 12.2 1.0 CG A:HIS87 3.1 10.4 1.0 CG A:HIS37 3.1 7.9 1.0 CB A:CYS84 3.2 11.6 1.0 CB A:HIS87 3.4 10.9 1.0 CB A:HIS37 3.5 9.9 1.0 CE A:MET92 3.6 9.5 1.0 CA A:HIS37 3.6 10.5 1.0 O A:PRO36 3.7 12.0 1.0 NE2 A:HIS37 4.1 9.0 1.0 CG A:MET92 4.1 9.7 1.0 NE2 A:HIS87 4.2 11.0 1.0 CD2 A:HIS87 4.2 12.5 1.0 CD2 A:HIS37 4.2 7.7 1.0 N A:HIS87 4.4 12.4 1.0 CG A:PRO86 4.4 14.9 1.0 C A:PRO36 4.5 12.4 1.0 N A:HIS37 4.5 10.3 1.0 CA A:HIS87 4.5 11.1 1.0 CB A:MET92 4.6 10.2 1.0 CA A:CYS84 4.6 10.6 1.0 N A:ASN38 4.7 10.1 1.0 C A:HIS37 4.8 10.2 1.0 O A:GLY34 4.9 12.0 1.0 C A:CYS84 5.0 11.7 1.0

Copper binding site 2 out of 2 in the Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure of the Spinach Plastocyanin Mutants G8D/K30C/T69C and K30C/T69C- A Study of the Effect on Crystal Packing and Thermostability From the Introduction of A Novel Disulfide Bond within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu402 b:12.1 occ:1.00 ND1 B:HIS37 2.0 8.0 1.0 ND1 B:HIS87 2.1 7.9 1.0 SG B:CYS84 2.2 11.3 1.0 SD B:MET92 2.6 10.8 1.0 CE1 B:HIS37 3.0 11.3 1.0 CE1 B:HIS87 3.0 10.5 1.0 CG B:HIS37 3.1 7.3 1.0 CG B:HIS87 3.1 9.7 1.0 CB B:CYS84 3.2 10.8 1.0 CE B:MET92 3.5 12.2 1.0 CB B:HIS37 3.5 9.6 1.0 CB B:HIS87 3.5 11.5 1.0 CA B:HIS37 3.7 9.5 1.0 CG B:MET92 3.9 12.3 1.0 NE2 B:HIS37 4.1 9.3 1.0 O B:PRO36 4.1 10.5 1.0 NE2 B:HIS87 4.1 8.1 1.0 CD2 B:HIS37 4.2 9.2 1.0 CD2 B:HIS87 4.2 8.7 1.0 CG B:PRO86 4.5 10.9 1.0 CB B:MET92 4.5 10.8 1.0 CA B:CYS84 4.6 10.6 1.0 N B:HIS87 4.7 10.7 1.0 N B:HIS37 4.7 9.7 1.0 CA B:HIS87 4.7 11.3 1.0 N B:ASN38 4.7 10.6 1.0 C B:HIS37 4.8 9.8 1.0 C B:PRO36 4.8 11.1 1.0

M.Okvist, F.Jacobson, H.Jansson, O.Hansson, L.Sjolin. Novel Disulfide Bonds Effect the Thermostability of Plastocyanin. Crystal Structures of the Triple Plastocyanin Mutant G8D/K30C/T69C and the Double Plastocyanin Mutant K30C/T69C From Spinach at 1.90 and 1.96 Resolution, Respectively. To Be Published.