Copper in PDB 1sfd: Oxidized Form of Amicyanin Mutant P94F

The structure of Oxidized Form of Amicyanin Mutant P94F, PDB code: 1sfd was solved by C.J.Carrell, D.Sun, S.Jiang, V.L.Davidson, F.S.Mathews, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 0.99
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.030, 59.500, 74.350, 90.00, 90.00, 90.00
R / Rfree (%)	11.6 / 14.7

The binding sites of Copper atom in the Oxidized Form of Amicyanin Mutant P94F (pdb code 1sfd). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Oxidized Form of Amicyanin Mutant P94F, PDB code: 1sfd:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the Oxidized Form of Amicyanin Mutant P94F


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Oxidized Form of Amicyanin Mutant P94F within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu107 b:6.2 occ:1.00 ND1 A:HIS53 2.0 4.7 1.0 ND1 A:HIS95 2.0 4.6 1.0 SG A:CYS92 2.2 5.1 1.0 SD A:MET98 2.8 5.8 1.0 CE1 A:HIS53 2.9 5.4 1.0 CE1 A:HIS95 3.0 5.2 1.0 CG A:HIS53 3.1 4.7 1.0 CG A:HIS95 3.1 5.0 1.0 CB A:CYS92 3.2 4.2 1.0 CB A:HIS95 3.4 4.3 1.0 CB A:HIS53 3.5 4.9 1.0 CE A:MET98 3.6 6.7 1.0 CA A:HIS53 3.7 4.2 1.0 O A:PRO52 3.8 5.8 1.0 NE2 A:HIS53 4.1 5.3 1.0 N A:HIS95 4.1 4.5 1.0 NE2 A:HIS95 4.2 4.8 1.0 CD2 A:HIS53 4.2 5.3 1.0 CD2 A:HIS95 4.2 5.1 1.0 CG A:MET98 4.3 8.3 1.0 CA A:HIS95 4.4 3.9 1.0 CB A:PHE94 4.4 5.6 1.0 CA A:CYS92 4.6 4.3 1.0 C A:PRO52 4.6 4.8 1.0 N A:HIS53 4.6 4.7 1.0 N A:ASN54 4.7 4.8 1.0 O A:HIS95 4.8 4.7 1.0 CD1 A:PHE94 4.8 10.6 1.0 C A:HIS53 4.8 4.5 1.0 CB A:MET98 4.8 8.6 1.0 C A:PHE94 4.9 4.3 1.0 CE A:MET28 5.0 8.5 1.0

Copper binding site 2 out of 2 in the Oxidized Form of Amicyanin Mutant P94F


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Oxidized Form of Amicyanin Mutant P94F within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu107 b:6.5 occ:1.00 ND1 B:HIS53 2.0 5.0 1.0 ND1 B:HIS95 2.0 5.2 1.0 SG B:CYS92 2.2 5.5 1.0 SD B:MET98 2.7 5.7 1.0 CE1 B:HIS53 2.9 5.7 1.0 CE1 B:HIS95 3.0 5.6 1.0 CG B:HIS95 3.1 4.9 1.0 CG B:HIS53 3.1 5.1 1.0 CB B:CYS92 3.2 5.2 1.0 CB B:HIS95 3.4 5.7 1.0 CB B:HIS53 3.5 5.2 1.0 CE B:MET98 3.6 7.2 1.0 CA B:HIS53 3.7 5.0 1.0 O B:PRO52 3.8 5.8 1.0 NE2 B:HIS53 4.1 6.1 1.0 N B:HIS95 4.1 5.4 1.0 NE2 B:HIS95 4.2 5.6 1.0 CG B:MET98 4.2 5.9 1.0 CD2 B:HIS95 4.2 5.8 1.0 CD2 B:HIS53 4.2 5.6 1.0 CA B:HIS95 4.4 4.9 1.0 CB B:PHE94 4.4 6.5 1.0 CA B:CYS92 4.6 4.2 1.0 C B:PRO52 4.6 5.5 1.0 N B:HIS53 4.7 4.9 1.0 N B:ASN54 4.7 4.8 1.0 CD1 B:PHE94 4.7 11.7 1.0 CB B:MET98 4.7 5.9 1.0 O B:HIS95 4.7 4.9 1.0 C B:HIS53 4.8 4.7 1.0 C B:PHE94 4.9 5.8 1.0 CE B:MET28 5.0 9.7 1.0

C.J.Carrell, D.Sun, S.Jiang, V.L.Davidson, F.S.Mathews. Structural Studies of Two Mutants of Amicyanin From Paracoccus Denitrificans That Stabilize the Reduced State of the Copper. Biochemistry V. 43 9372 2004.
ISSN: ISSN 0006-2960
PubMed: 15260480
DOI: 10.1021/BI049634Z