Copper in the structure of Peptidylglycine Alpha-Hydroxylating Monooxygenase (Phm) From Rat (pdb 1phm)

The binding sites of Copper atom in the structure of Peptidylglycine Alpha-Hydroxylating Monooxygenase (Phm) From Rat (pdb code 1phm). This binding sites where shown with 5.0 Angstroms radius around Copper atom. The 1phm structure was solved by S.T.PRIGGE, L.M.AMZEL, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 6.0-1.9 Space group P212121 a (A) 68.400 b (A) 68.660 c (A) 81.380 alpha (°) 90.00 beta (°) 90.00 gamma (°) 90.00 Rfactor (%) 19.6 Rfree (%) 26.1 Copper Binding Sites: Copper binding site 1 out of 3 in 1phm Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Copper in the PDB 1phm. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Tyr79, A: His107, A: His108, A: His172, A: Hoh438, A: Hoh588, conact list: Atom Atom Distance (A) Cu OH A:Tyr79 4.71 Cu O A:His107 4.14 Cu NE2 A:His107 4.08 Cu CB A:His107 3.26 Cu ND1 A:His107 1.92 Cu CD2 A:His107 4.09 Cu C A:His107 3.70 Cu CE1 A:His107 2.94 Cu CG A:His107 2.93 Cu CA A:His107 4.11 Cu O A:His108 4.93 Cu NE2 A:His108 4.08 Cu N A:His108 3.50 Cu CB A:His108 3.26 Cu ND1 A:His108 1.92 Cu CD2 A:His108 4.08 Cu C A:His108 4.86 Cu CE1 A:His108 2.94 Cu CG A:His108 2.93 Cu CA A:His108 3.98 Cu NE2 A:His172 4.37 Cu CB A:His172 3.75 Cu ND1 A:His172 2.29 Cu CD2 A:His172 4.47 Cu CE1 A:His172 3.17 Cu CG A:His172 3.37 Cu O A:Hoh438 4.02 Cu O A:Hoh588 4.88 interactive model: Copper binding site 2 out of 3 in 1phm Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Copper in the PDB 1phm. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: His242, A: His244, A: Gly307, A: Met314, A: Hoh360, A: Hoh594, conact list: Atom Atom Distance (A) Cu NE2 A:His242 1.91 Cu ND1 A:His242 4.06 Cu CD2 A:His242 2.94 Cu CE1 A:His242 2.92 Cu CG A:His242 4.09 Cu NE2 A:His244 2.09 Cu ND1 A:His244 4.24 Cu CD2 A:His244 3.03 Cu CE1 A:His244 3.14 Cu CG A:His244 4.22 Cu O A:Gly307 4.87 Cu CB A:Met314 3.69 Cu CE A:Met314 3.62 Cu CG A:Met314 3.34 Cu SD A:Met314 2.42 Cu O A:Hoh360 2.28 Cu O A:Hoh594 4.48 interactive model: Copper binding site 3 out of 3 in 1phm Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 3 of Copper in the PDB 1phm. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: His235, A: Asp282, A: Azi361, A: Gol362, A: Hoh363, A: Hoh370, A: Hoh424, A: Hoh525, conact list: Atom Atom Distance (A) Cu NE2 A:His235 2.08 Cu ND1 A:His235 4.22 Cu CD2 A:His235 2.93 Cu CE1 A:His235 3.17 Cu CG A:His235 4.14 Cu CB A:Asp282 4.08 Cu OD1 A:Asp282 4.45 Cu CG A:Asp282 4.53 Cu N1 A:Azi361 1.84 Cu N3 A:Azi361 3.95 Cu N2 A:Azi361 2.81 Cu O2 A:Gol362 2.28 Cu C1 A:Gol362 2.80 Cu O1 A:Gol362 2.26 Cu C3 A:Gol362 4.33 Cu C2 A:Gol362 2.90 Cu O A:Hoh363 2.01 Cu O A:Hoh370 4.53 Cu O A:Hoh424 4.47 Cu O A:Hoh525 4.62 interactive model: