Copper in the structure of The Crystal Structure of the Spinach Plastocyanin Double Mutant G8D/L12E Gives Insight Into Its Low Reactivity Towards Photosystem 1 and Cytochrome F (pdb 1oow)

The binding sites of Copper atom in the structure of The Crystal Structure of the Spinach Plastocyanin Double Mutant G8D/L12E Gives Insight Into Its Low Reactivity Towards Photosystem 1 and Cytochrome F (pdb code 1oow). This binding sites where shown with 5.0 Angstroms radius around Copper atom. The 1oow structure was solved by H.JANSSON, M.OKVIST, F.JACOBSON, M.EJDEBACK, O.HANSSON, L.SJOLIN, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 19.3-2.0 Space group P3121 a (A) 74.761 b (A) 74.761 c (A) 31.383 alpha (°) 90.00 beta (°) 90.00 gamma (°) 120.00 Rfactor (%) 18.3 Rfree (%) 23.2 Copper Binding Sites: Copper binding site 1 out of 1 in 1oow Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Copper in the PDB 1oow. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Glu12, A: Pro36, A: His37, A: Asn38, A: Cys84, A: Pro86, A: His87, A: Met92, conact list: Atom Atom Distance (A) Cu OE1 A:Glu12 3.85 Cu CD A:Glu12 4.85 Cu O A:Pro36 3.58 Cu C A:Pro36 4.45 Cu NE2 A:His37 4.07 Cu N A:His37 4.57 Cu CB A:His37 3.75 Cu ND1 A:His37 2.11 Cu CD2 A:His37 4.27 Cu C A:His37 4.91 Cu CE1 A:His37 2.87 Cu CG A:His37 3.26 Cu CA A:His37 3.80 Cu N A:Asn38 4.83 Cu CB A:Cys84 3.22 Cu SG A:Cys84 2.22 Cu C A:Cys84 4.98 Cu CA A:Cys84 4.60 Cu CB A:Pro86 4.84 Cu CG A:Pro86 4.82 Cu NE2 A:His87 4.21 Cu N A:His87 4.34 Cu CB A:His87 3.44 Cu ND1 A:His87 2.18 Cu CD2 A:His87 4.24 Cu CE1 A:His87 3.11 Cu CG A:His87 3.13 Cu CA A:His87 4.53 Cu CB A:Met92 4.64 Cu CE A:Met92 3.62 Cu CG A:Met92 4.33 Cu SD A:Met92 2.89 interactive model: