Copper in the structure of Crystallographic Study of Azurin From Pseudomonas Putida (pdb 1nwo)

The binding sites of Copper atom in the structure of Crystallographic Study of Azurin From Pseudomonas Putida (pdb code 1nwo). This binding sites where shown with 5.0 Angstroms radius around Copper atom. The 1nwo structure was solved by F.S.MATHEWS, Z.-W.CHEN, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 8.0-1.9 Space group P1211 a (A) 43.250 b (A) 50.650 c (A) 54.600 alpha (°) 90.00 beta (°) 107.79 gamma (°) 90.00 Rfactor (%) 16.9 Rfree (%) 25.7 Copper Binding Sites: Copper binding site 1 out of 2 in 1nwo Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Copper in the PDB 1nwo. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: A: Gly45, A: His46, A: Asn47, A: Cys112, A: Phe114, A: His117, A: Met121, conact list: Atom Atom Distance (A) Cu O A:Gly45 3.16 Cu C A:Gly45 4.08 Cu NE2 A:His46 4.14 Cu N A:His46 4.28 Cu CB A:His46 3.57 Cu ND1 A:His46 2.01 Cu CD2 A:His46 4.22 Cu C A:His46 4.66 Cu CE1 A:His46 2.94 Cu CG A:His46 3.14 Cu CA A:His46 3.53 Cu N A:Asn47 4.66 Cu CB A:Cys112 3.28 Cu SG A:Cys112 2.13 Cu CA A:Cys112 4.68 Cu N A:Phe114 4.89 Cu CB A:Phe114 3.86 Cu CG A:Phe114 4.70 Cu CA A:Phe114 4.95 Cu NE2 A:His117 4.06 Cu CB A:His117 3.40 Cu ND1 A:His117 1.94 Cu CD2 A:His117 4.11 Cu CE1 A:His117 2.88 Cu CG A:His117 3.00 Cu CA A:His117 4.89 Cu CB A:Met121 4.91 Cu CE A:Met121 3.69 Cu CG A:Met121 4.47 Cu SD A:Met121 3.01 interactive model: Copper binding site 2 out of 2 in 1nwo Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 2 of Copper in the PDB 1nwo. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Copper atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: B: Gly45, B: His46, B: Asn47, B: Cys112, B: Phe114, B: His117, B: Met121, conact list: Atom Atom Distance (A) Cu O B:Gly45 2.95 Cu C B:Gly45 3.88 Cu NE2 B:His46 4.04 Cu N B:His46 4.13 Cu CB B:His46 3.54 Cu ND1 B:His46 1.93 Cu CD2 B:His46 4.14 Cu C B:His46 4.64 Cu CE1 B:His46 2.84 Cu CG B:His46 3.07 Cu CA B:His46 3.45 Cu N B:Asn47 4.70 Cu CB B:Cys112 3.28 Cu SG B:Cys112 2.14 Cu CA B:Cys112 4.70 Cu N B:Phe114 4.89 Cu CB B:Phe114 3.89 Cu CG B:Phe114 4.73 Cu CA B:Phe114 4.98 Cu NE2 B:His117 4.09 Cu CB B:His117 3.29 Cu ND1 B:His117 1.96 Cu CD2 B:His117 4.08 Cu CE1 B:His117 2.94 Cu CG B:His117 2.96 Cu CA B:His117 4.79 Cu CE B:Met121 3.93 Cu CG B:Met121 4.62 Cu SD B:Met121 3.14 interactive model: