Copper in PDB 1n70: The Crystal Structure of Nitrite Reductase Mutant HIS287ALA From Rhodobacter Sphaeroides

All present enzymatic activity of The Crystal Structure of Nitrite Reductase Mutant HIS287ALA From Rhodobacter Sphaeroides:
1.7.99.3;

The structure of The Crystal Structure of Nitrite Reductase Mutant HIS287ALA From Rhodobacter Sphaeroides, PDB code: 1n70 was solved by H.Guo, K.Olesen, J.Shapliegh, L.Sjolin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	13.25 / 1.60
Space group	H 3
Cell size a, b, c (Å), α, β, γ (°)	71.395, 71.395, 146.179, 90.00, 90.00, 120.00
R / Rfree (%)	17.4 / 20.2

The structure of The Crystal Structure of Nitrite Reductase Mutant HIS287ALA From Rhodobacter Sphaeroides also contains other interesting chemical elements:

Magnesium

(Mg)

3 atoms

The binding sites of Copper atom in the The Crystal Structure of Nitrite Reductase Mutant HIS287ALA From Rhodobacter Sphaeroides (pdb code 1n70). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the The Crystal Structure of Nitrite Reductase Mutant HIS287ALA From Rhodobacter Sphaeroides, PDB code: 1n70:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the The Crystal Structure of Nitrite Reductase Mutant HIS287ALA From Rhodobacter Sphaeroides


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of The Crystal Structure of Nitrite Reductase Mutant HIS287ALA From Rhodobacter Sphaeroides within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu401 b:13.9 occ:1.00 ND1 A:HIS126 2.0 9.8 1.0 ND1 A:HIS177 2.1 14.9 1.0 SG A:CYS167 2.2 12.6 1.0 SD A:MET182 2.5 17.2 1.0 CE1 A:HIS126 3.0 12.9 1.0 CE1 A:HIS177 3.0 16.4 1.0 CG A:HIS126 3.1 12.4 1.0 CG A:HIS177 3.1 13.9 1.0 CB A:CYS167 3.2 13.5 1.0 CE A:MET182 3.4 17.0 1.0 CB A:HIS126 3.5 12.1 1.0 CB A:HIS177 3.5 12.7 1.0 CA A:HIS126 3.9 12.6 1.0 CG A:MET182 4.0 18.2 1.0 NE2 A:HIS126 4.1 12.7 1.0 NE2 A:HIS177 4.1 14.4 1.0 CD2 A:HIS126 4.2 12.0 1.0 CD2 A:HIS177 4.2 15.6 1.0 O A:PRO125 4.2 13.9 1.0 CG A:PRO169 4.2 15.1 1.0 CB A:MET182 4.4 14.5 1.0 CA A:CYS167 4.6 12.4 1.0 N A:ASN127 4.7 11.9 1.0 CD A:PRO169 4.7 15.5 1.0 CE A:MET93 4.7 13.4 1.0 CA A:HIS177 4.8 12.2 1.0 C A:HIS126 4.8 12.2 1.0 N A:HIS126 4.9 12.5 1.0 C A:PRO125 5.0 14.5 1.0

Copper binding site 2 out of 2 in the The Crystal Structure of Nitrite Reductase Mutant HIS287ALA From Rhodobacter Sphaeroides


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of The Crystal Structure of Nitrite Reductase Mutant HIS287ALA From Rhodobacter Sphaeroides within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu402 b:14.7 occ:1.00 NE2 A:HIS338 2.2 12.3 1.0 CE1 A:HIS338 3.0 11.6 1.0 CD2 A:HIS338 3.3 13.2 1.0 ND1 A:HIS338 4.2 11.0 1.0 CG A:HIS338 4.3 12.5 1.0 O A:HOH828 4.5 29.8 1.0 CG1 A:ILE289 4.7 16.6 1.0 O A:HOH674 4.8 19.5 1.0 CD1 A:ILE289 4.8 20.7 1.0

H.Guo, K.Olesen, J.Shapliegh, L.Sjolin. The Crystal Structure of Nitrite Reductase Mutant HIS287ALA From Rhodobacter Sphaeroides to 1.6 Resolution To Be Published.