Copper in PDB 1kcb: Crystal Structure of A No-Forming Nitrite Reductase Mutant: An Analog of A Transition State in Enzymatic Reaction

All present enzymatic activity of Crystal Structure of A No-Forming Nitrite Reductase Mutant: An Analog of A Transition State in Enzymatic Reaction:
1.7.99.3;

The structure of Crystal Structure of A No-Forming Nitrite Reductase Mutant: An Analog of A Transition State in Enzymatic Reaction, PDB code: 1kcb was solved by S.Q.Liu, T.Chang, M.Y.Liu, J.Legall, W.C.Chang, J.P.Zhang, D.C.Liang, W.R.Chang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	9.97 / 1.65
Space group	P 21 3
Cell size a, b, c (Å), α, β, γ (°)	98.160, 98.160, 98.160, 90.00, 90.00, 90.00
R / Rfree (%)	17 / 20.4

The binding sites of Copper atom in the Crystal Structure of A No-Forming Nitrite Reductase Mutant: An Analog of A Transition State in Enzymatic Reaction (pdb code 1kcb). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Crystal Structure of A No-Forming Nitrite Reductase Mutant: An Analog of A Transition State in Enzymatic Reaction, PDB code: 1kcb:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the Crystal Structure of A No-Forming Nitrite Reductase Mutant: An Analog of A Transition State in Enzymatic Reaction


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure of A No-Forming Nitrite Reductase Mutant: An Analog of A Transition State in Enzymatic Reaction within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu351 b:18.6 occ:1.00 ND1 A:HIS145 2.1 16.1 1.0 ND1 A:HIS95 2.2 14.6 1.0 SG A:CYS136 2.2 17.0 1.0 SD A:MET150 2.6 16.2 1.0 CE1 A:HIS145 3.0 17.5 1.0 CE1 A:HIS95 3.1 13.1 1.0 CG A:HIS145 3.2 15.1 1.0 CB A:CYS136 3.2 17.4 1.0 CG A:HIS95 3.2 15.4 1.0 CE A:MET150 3.4 14.1 1.0 CB A:HIS145 3.5 12.8 1.0 CB A:HIS95 3.6 15.7 1.0 CG A:MET150 3.9 13.5 1.0 CA A:HIS95 4.0 14.7 1.0 NE2 A:HIS145 4.2 16.0 1.0 CD2 A:HIS145 4.3 15.9 1.0 NE2 A:HIS95 4.3 13.5 1.0 O A:LEU94 4.3 15.6 1.0 CB A:MET150 4.4 12.8 1.0 CD2 A:HIS95 4.4 12.4 1.0 CG A:PRO138 4.4 16.5 1.0 SD A:MET62 4.5 18.2 1.0 CA A:CYS136 4.6 12.7 1.0 CA A:HIS145 4.7 12.5 1.0 N A:ASN96 4.7 14.7 1.0 CD A:PRO138 4.8 16.5 1.0 CB A:MET62 4.9 14.8 1.0 C A:HIS95 4.9 16.1 1.0 N A:HIS95 5.0 15.8 1.0

Copper binding site 2 out of 2 in the Crystal Structure of A No-Forming Nitrite Reductase Mutant: An Analog of A Transition State in Enzymatic Reaction


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure of A No-Forming Nitrite Reductase Mutant: An Analog of A Transition State in Enzymatic Reaction within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu352 b:13.3 occ:1.00 NE2 A:HIS100 2.1 12.0 1.0 NE2 A:HIS135 2.2 12.8 1.0 CE1 A:HIS100 3.1 11.0 1.0 CD2 A:HIS100 3.1 9.2 1.0 CE1 A:HIS135 3.2 11.3 1.0 CD2 A:HIS135 3.2 11.8 1.0 OD2 A:ASP98 3.8 19.7 1.0 ND1 A:HIS100 4.2 10.4 1.0 CG A:HIS100 4.3 9.3 1.0 ND1 A:HIS135 4.3 13.8 1.0 CG A:HIS135 4.3 11.6 1.0 CG A:ASP98 4.4 16.8 1.0 OD1 A:ASP98 4.7 12.9 1.0

S.Q.Liu, T.Chang, M.Y.Liu, J.Legall, W.C.Chang, J.P.Zhang, D.C.Liang, W.R.Chang. Crystal Structure of A No-Forming Nitrite Reductase Mutant: An Analog of A Transition State in Enzymatic Reaction Biochem.Biophys.Res.Commun. V. 302 568 2003.
ISSN: ISSN 0006-291X
PubMed: 12615072
DOI: 10.1016/S0006-291X(03)00166-9