Copper in PDB 1gy2: Crystal Structure of MET148LEU Rusticyanin

The structure of Crystal Structure of MET148LEU Rusticyanin, PDB code: 1gy2 was solved by M.A.Hough, L.D.Kanbi, S.Antonyuk, F.Dodd, S.Hasnain, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	53.4 / 1.82
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	43.450, 61.430, 53.470, 90.00, 96.31, 90.00
R / Rfree (%)	18.3 / 21.9

The binding sites of Copper atom in the Crystal Structure of MET148LEU Rusticyanin (pdb code 1gy2). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Crystal Structure of MET148LEU Rusticyanin, PDB code: 1gy2:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the Crystal Structure of MET148LEU Rusticyanin


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure of MET148LEU Rusticyanin within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu1156 b:11.8 occ:1.00 ND1 A:HIS143 2.0 11.0 1.0 ND1 A:HIS85 2.1 9.9 1.0 SG A:CYS138 2.2 10.8 1.0 CE1 A:HIS85 3.0 11.9 1.0 CE1 A:HIS143 3.0 12.2 1.0 CG A:HIS143 3.0 11.6 1.0 CD2 A:LEU148 3.1 11.6 1.0 CG A:HIS85 3.1 10.7 1.0 CB A:CYS138 3.3 10.7 1.0 CB A:HIS143 3.4 11.0 1.0 CD1 A:ILE140 3.5 12.6 1.0 CB A:HIS85 3.5 10.5 1.0 CB A:ILE140 3.6 12.6 1.0 CG1 A:ILE140 3.7 12.9 1.0 CA A:HIS85 3.8 11.3 1.0 NE2 A:HIS143 4.1 13.5 1.0 NE2 A:HIS85 4.1 11.0 1.0 CD2 A:HIS143 4.2 13.1 1.0 CD2 A:HIS85 4.2 11.3 1.0 CG2 A:ILE140 4.5 10.6 1.0 CG A:LEU148 4.5 11.2 1.0 N A:ILE140 4.6 12.9 1.0 N A:HIS85 4.6 11.8 1.0 CA A:CYS138 4.7 10.6 1.0 CA A:ILE140 4.7 12.9 1.0 N A:SER86 4.7 10.7 1.0 C A:HIS85 4.8 10.8 1.0 CA A:HIS143 4.9 10.8 1.0

Copper binding site 2 out of 2 in the Crystal Structure of MET148LEU Rusticyanin


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure of MET148LEU Rusticyanin within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu1156 b:13.2 occ:1.00 ND1 B:HIS143 2.0 11.8 1.0 ND1 B:HIS85 2.1 12.4 1.0 SG B:CYS138 2.2 12.3 1.0 CE1 B:HIS85 3.0 12.8 1.0 CE1 B:HIS143 3.0 13.7 1.0 CG B:HIS143 3.0 13.2 1.0 CD2 B:LEU148 3.1 12.3 1.0 CG B:HIS85 3.1 11.2 1.0 CB B:CYS138 3.2 12.4 1.0 CB B:HIS143 3.4 12.0 1.0 CD1 B:ILE140 3.5 15.4 1.0 CB B:HIS85 3.5 12.0 1.0 CB B:ILE140 3.7 13.6 1.0 CA B:HIS85 3.7 11.6 1.0 CG1 B:ILE140 3.8 14.0 1.0 NE2 B:HIS143 4.1 13.5 1.0 NE2 B:HIS85 4.1 11.0 1.0 CD2 B:HIS143 4.1 14.4 1.0 CD2 B:HIS85 4.2 11.7 1.0 CG B:LEU148 4.4 12.4 1.0 N B:ILE140 4.6 13.3 1.0 CA B:CYS138 4.6 12.0 1.0 CG2 B:ILE140 4.6 14.8 1.0 N B:HIS85 4.7 11.7 1.0 N B:SER86 4.7 11.4 1.0 CA B:ILE140 4.7 13.7 1.0 C B:HIS85 4.8 11.2 1.0 CA B:HIS143 4.9 12.2 1.0 C B:CYS138 5.0 12.3 1.0

L.D.Kanbi, S.Antonyuk, M.A.Hough, J.F.Hall, F.Dodd, S.Hasnain. Crystal Structures of the MET148LEU and SER86ASP Mutants of Rusticyanin From Thiobacillus Ferrooxidans: Insights Into the Structural Relationship with the Cupredoxins and the Multi Copper Proteins. J.Mol.Biol. V. 320 263 2002.
ISSN: ISSN 0022-2836
PubMed: 12079384
DOI: 10.1016/S0022-2836(02)00443-6