Copper in PDB 1gy1: Crystal Structures of SER86ASP and MET148LEU Rusticyanin

The structure of Crystal Structures of SER86ASP and MET148LEU Rusticyanin, PDB code: 1gy1 was solved by M.A.Hough, L.D.Kanbi, S.Antonyuk, F.Dodd, S.Hasnain, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	52.70 / 1.65
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	43.098, 61.408, 53.346, 90.00, 96.13, 90.00
R / Rfree (%)	16.6 / 19

The binding sites of Copper atom in the Crystal Structures of SER86ASP and MET148LEU Rusticyanin (pdb code 1gy1). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Crystal Structures of SER86ASP and MET148LEU Rusticyanin, PDB code: 1gy1:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the Crystal Structures of SER86ASP and MET148LEU Rusticyanin


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structures of SER86ASP and MET148LEU Rusticyanin within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu1156 b:19.8 occ:1.00 ND1 A:HIS143 2.1 16.0 1.0 ND1 A:HIS85 2.1 15.5 1.0 SG A:CYS138 2.2 16.1 1.0 SD A:MET148 2.8 16.2 1.0 CE1 A:HIS85 3.0 16.2 1.0 CE1 A:HIS143 3.0 19.3 1.0 CG A:HIS143 3.1 16.8 1.0 CB A:CYS138 3.2 15.4 1.0 CG A:HIS85 3.2 14.7 1.0 CB A:HIS143 3.4 14.4 1.0 CE A:MET148 3.6 17.3 1.0 CB A:HIS85 3.6 15.2 1.0 CD1 A:ILE140 3.7 20.2 1.0 CB A:ILE140 4.0 16.9 1.0 CG1 A:ILE140 4.0 18.3 1.0 CA A:HIS85 4.1 15.3 1.0 NE2 A:HIS85 4.1 14.9 1.0 NE2 A:HIS143 4.1 18.0 1.0 CG A:MET148 4.2 16.1 1.0 CD2 A:HIS143 4.2 18.3 1.0 CD2 A:HIS85 4.3 14.7 1.0 CA A:CYS138 4.6 14.9 1.0 CB A:MET148 4.7 15.6 1.0 N A:ILE140 4.8 15.4 1.0 CA A:HIS143 4.9 14.7 1.0 CG2 A:ILE140 4.9 17.5 1.0 N A:HIS85 5.0 16.2 1.0 CA A:ILE140 5.0 16.1 1.0 N A:ASP86 5.0 15.3 1.0

Copper binding site 2 out of 2 in the Crystal Structures of SER86ASP and MET148LEU Rusticyanin


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structures of SER86ASP and MET148LEU Rusticyanin within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu1156 b:17.8 occ:1.00 ND1 B:HIS85 2.1 13.7 1.0 ND1 B:HIS143 2.1 14.4 1.0 SG B:CYS138 2.2 15.2 1.0 SD B:MET148 2.8 15.2 1.0 CE1 B:HIS85 2.9 13.5 1.0 CE1 B:HIS143 3.1 19.0 1.0 CG B:HIS143 3.1 16.4 1.0 CB B:CYS138 3.2 14.6 1.0 CG B:HIS85 3.2 13.8 1.0 CB B:HIS143 3.4 15.0 1.0 CE B:MET148 3.5 15.3 1.0 CB B:HIS85 3.6 12.8 1.0 CD1 B:ILE140 3.9 18.4 1.0 CB B:ILE140 4.0 15.5 1.0 CA B:HIS85 4.0 13.2 1.0 CG1 B:ILE140 4.1 16.4 1.0 NE2 B:HIS85 4.1 14.4 1.0 CG B:MET148 4.2 14.2 1.0 NE2 B:HIS143 4.2 17.4 1.0 CD2 B:HIS143 4.2 18.5 1.0 CD2 B:HIS85 4.2 14.9 1.0 CA B:CYS138 4.6 13.7 1.0 N B:ILE140 4.7 14.4 1.0 CB B:MET148 4.7 13.0 1.0 CG2 B:ILE140 4.9 16.2 1.0 N B:HIS85 4.9 13.2 1.0 CA B:HIS143 4.9 14.9 1.0 CA B:ILE140 4.9 15.6 1.0 N B:ASP86 5.0 13.3 1.0

L.D.Kanbi, S.Antonyuk, M.A.Hough, J.Hall, F.Dodd, S.Hasnain. Crystal Structures of the MET148LEU and SER86ASP Mutants of Rusticyanin From Thiobacillus Ferrooxidans: Insights Into the Structural Relationship with the Cupredoxins and the Multi Copper Proteins J.Mol.Biol. V. 320 263 2002.
ISSN: ISSN 0022-2836
PubMed: 12079384
DOI: 10.1016/S0022-2836(02)00443-6