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Copper in PDB 1ezl: Crystal Structure of the Disulphide Bond-Deficient Azurin Mutant C3A/C26A: How Important Is the S-S Bond For Folding and Stability?

Protein crystallography data

The structure of Crystal Structure of the Disulphide Bond-Deficient Azurin Mutant C3A/C26A: How Important Is the S-S Bond For Folding and Stability?, PDB code: 1ezl was solved by N.Bonander, J.Leckner, H.Guo, B.G.Karlsson, L.Sjolin, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 8.00 / 2.00
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 57.400, 80.400, 110.300, 90.00, 90.00, 90.00
R / Rfree (%) 18.9 / 25.6

Copper Binding Sites:

The binding sites of Copper atom in the Crystal Structure of the Disulphide Bond-Deficient Azurin Mutant C3A/C26A: How Important Is the S-S Bond For Folding and Stability? (pdb code 1ezl). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 4 binding sites of Copper where determined in the Crystal Structure of the Disulphide Bond-Deficient Azurin Mutant C3A/C26A: How Important Is the S-S Bond For Folding and Stability?, PDB code: 1ezl:
Jump to Copper binding site number: 1; 2; 3; 4;

Copper binding site 1 out of 4 in 1ezl

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Copper binding site 1 out of 4 in the Crystal Structure of the Disulphide Bond-Deficient Azurin Mutant C3A/C26A: How Important Is the S-S Bond For Folding and Stability?


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure of the Disulphide Bond-Deficient Azurin Mutant C3A/C26A: How Important Is the S-S Bond For Folding and Stability? within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu1001

b:9.7
occ:1.00
ND1 A:HIS46 2.2 13.1 1.0
SG A:CYS112 2.3 16.4 1.0
O A:GLY45 2.4 16.3 1.0
ND1 A:HIS117 2.4 2.0 1.0
CE1 A:HIS46 2.9 11.5 1.0
CG A:HIS46 3.3 7.4 1.0
CG A:HIS117 3.3 9.8 1.0
CE1 A:HIS117 3.4 4.1 1.0
SD A:MET121 3.4 13.0 1.0
C A:GLY45 3.5 9.3 1.0
CA A:HIS46 3.5 10.2 1.0
CB A:HIS117 3.6 10.7 1.0
CB A:CYS112 3.6 14.7 1.0
CB A:HIS46 3.8 13.2 1.0
CB A:PHE114 3.9 11.0 1.0
N A:HIS46 4.0 11.3 1.0
CE A:MET121 4.1 2.0 1.0
NE2 A:HIS46 4.1 12.1 1.0
CD2 A:HIS46 4.3 7.5 1.0
NE2 A:HIS117 4.4 11.7 1.0
CD2 A:HIS117 4.5 9.0 1.0
CG A:PHE114 4.6 8.2 1.0
CG A:MET121 4.8 2.0 1.0
C A:HIS46 4.8 7.2 1.0
CA A:GLY45 4.8 8.7 1.0
N A:ASN47 4.9 2.0 1.0
N A:PHE114 4.9 9.2 1.0
CA A:CYS112 5.0 3.5 1.0
N A:GLY45 5.0 10.7 1.0
CA A:PHE114 5.0 6.9 1.0

Copper binding site 2 out of 4 in 1ezl

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Copper binding site 2 out of 4 in the Crystal Structure of the Disulphide Bond-Deficient Azurin Mutant C3A/C26A: How Important Is the S-S Bond For Folding and Stability?


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure of the Disulphide Bond-Deficient Azurin Mutant C3A/C26A: How Important Is the S-S Bond For Folding and Stability? within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu1001

b:7.0
occ:1.00
ND1 B:HIS247 2.2 16.4 1.0
ND1 B:HIS176 2.2 11.8 1.0
SG B:CYS242 2.4 4.4 1.0
O B:GLY175 2.5 12.0 1.0
CE1 B:HIS247 3.0 12.7 1.0
CE1 B:HIS176 3.0 6.4 1.0
SD B:MET251 3.2 10.5 1.0
CG B:HIS247 3.3 14.9 1.0
CB B:CYS242 3.3 2.8 1.0
CG B:HIS176 3.4 12.9 1.0
CA B:HIS176 3.5 7.0 1.0
C B:GLY175 3.6 5.5 1.0
CB B:HIS247 3.7 8.9 1.0
CB B:HIS176 3.8 9.8 1.0
CE B:MET251 3.9 2.0 1.0
CB B:PHE244 3.9 13.0 1.0
N B:HIS176 4.0 2.0 1.0
NE2 B:HIS247 4.2 14.4 1.0
NE2 B:HIS176 4.2 14.0 1.0
CD2 B:HIS247 4.3 12.8 1.0
CD2 B:HIS176 4.4 12.5 1.0
CG B:MET251 4.5 2.0 1.0
C B:HIS176 4.6 7.2 1.0
N B:ASN177 4.6 9.4 1.0
CG B:PHE244 4.7 15.3 1.0
CA B:CYS242 4.8 6.9 1.0
CB B:MET251 4.8 2.0 1.0
N B:PHE244 4.9 15.2 1.0
CA B:GLY175 4.9 11.7 1.0
CA B:PHE244 5.0 12.8 1.0

Copper binding site 3 out of 4 in 1ezl

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Copper binding site 3 out of 4 in the Crystal Structure of the Disulphide Bond-Deficient Azurin Mutant C3A/C26A: How Important Is the S-S Bond For Folding and Stability?


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Crystal Structure of the Disulphide Bond-Deficient Azurin Mutant C3A/C26A: How Important Is the S-S Bond For Folding and Stability? within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cu1001

b:2.0
occ:1.00
SG C:CYS372 2.2 14.8 1.0
ND1 C:HIS377 2.2 7.2 1.0
ND1 C:HIS306 2.3 12.9 1.0
SD C:MET381 3.1 13.9 1.0
CG C:HIS377 3.1 9.5 1.0
CG C:HIS306 3.1 8.5 1.0
CE1 C:HIS306 3.2 2.0 1.0
CE1 C:HIS377 3.2 11.2 1.0
O C:GLY305 3.2 15.6 1.0
CB C:CYS372 3.3 12.0 1.0
CB C:HIS377 3.3 5.4 1.0
CA C:HIS306 3.4 5.3 1.0
CB C:HIS306 3.4 8.6 1.0
CE C:MET381 3.8 4.0 1.0
CB C:PHE374 3.9 2.0 1.0
C C:GLY305 4.0 12.0 1.0
N C:HIS306 4.1 10.6 1.0
CD2 C:HIS306 4.2 8.6 1.0
NE2 C:HIS306 4.2 2.0 1.0
CD2 C:HIS377 4.2 12.2 1.0
NE2 C:HIS377 4.3 13.2 1.0
CG C:MET381 4.5 13.0 1.0
CA C:CYS372 4.7 6.7 1.0
CG C:PHE374 4.7 2.0 1.0
C C:HIS306 4.7 8.9 1.0
N C:PHE374 4.8 8.4 1.0
CA C:HIS377 4.8 7.2 1.0
N C:ASN307 4.8 8.8 1.0
CB C:MET381 4.9 8.5 1.0
CA C:PHE374 4.9 3.1 1.0

Copper binding site 4 out of 4 in 1ezl

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Copper binding site 4 out of 4 in the Crystal Structure of the Disulphide Bond-Deficient Azurin Mutant C3A/C26A: How Important Is the S-S Bond For Folding and Stability?


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Crystal Structure of the Disulphide Bond-Deficient Azurin Mutant C3A/C26A: How Important Is the S-S Bond For Folding and Stability? within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Cu1001

b:8.9
occ:1.00
SG D:CYS502 2.2 19.0 1.0
ND1 D:HIS507 2.5 14.8 1.0
O D:GLY435 2.7 13.7 1.0
ND1 D:HIS436 3.0 28.2 1.0
SD D:MET511 3.2 13.5 1.0
CG D:HIS507 3.2 19.8 1.0
CB D:HIS507 3.3 17.0 1.0
CE1 D:HIS507 3.5 17.7 1.0
CB D:PHE504 3.7 13.8 1.0
CB D:CYS502 3.7 14.4 1.0
CE1 D:HIS436 3.7 27.4 1.0
C D:GLY435 3.8 15.3 1.0
CA D:HIS436 3.9 16.8 1.0
CE D:MET511 4.0 18.1 1.0
CG D:HIS436 4.1 25.6 1.0
N D:HIS436 4.3 14.7 1.0
CG D:PHE504 4.4 14.2 1.0
CD2 D:HIS507 4.4 18.0 1.0
CB D:HIS436 4.4 18.9 1.0
NE2 D:HIS507 4.5 19.7 1.0
CG D:MET511 4.6 11.2 1.0
N D:PHE504 4.7 15.4 1.0
CA D:PHE504 4.8 14.4 1.0
CA D:HIS507 4.8 12.6 1.0
CA D:CYS502 4.9 11.7 1.0
NE2 D:HIS436 5.0 27.6 1.0
N D:ASN437 5.0 12.0 1.0
C D:HIS436 5.0 14.6 1.0

Reference:

N.Bonander, J.Leckner, H.Guo, B.G.Karlsson, L.Sjolin. Crystal Structure of the Disulfide Bond-Deficient Azurin Mutant C3A/C26A: How Important Is the S-S Bond For Folding and Stability? Eur.J.Biochem. V. 267 4511 2000.
ISSN: ISSN 0014-2956
PubMed: 10880975
Page generated: Wed Sep 2 21:42:17 2020
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