Atomistry » Copper » PDB 1bex-1eqw » 1ekj
Atomistry »
  Copper »
    PDB 1bex-1eqw »
      1ekj »

Copper in PDB 1ekj: The X-Ray Crystallographic Structure of Beta Carbonic Anhydrase From the C3 Dicot Pisum Sativum

Enzymatic activity of The X-Ray Crystallographic Structure of Beta Carbonic Anhydrase From the C3 Dicot Pisum Sativum

All present enzymatic activity of The X-Ray Crystallographic Structure of Beta Carbonic Anhydrase From the C3 Dicot Pisum Sativum:
4.2.1.1;

Protein crystallography data

The structure of The X-Ray Crystallographic Structure of Beta Carbonic Anhydrase From the C3 Dicot Pisum Sativum, PDB code: 1ekj was solved by M.S.Kimber, E.F.Pai, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 40.00 / 1.93
Space group C 2 2 2
Cell size a, b, c (Å), α, β, γ (°) 136.909, 143.318, 202.135, 90.00, 90.00, 90.00
R / Rfree (%) 22.9 / 25

Other elements in 1ekj:

The structure of The X-Ray Crystallographic Structure of Beta Carbonic Anhydrase From the C3 Dicot Pisum Sativum also contains other interesting chemical elements:

Chlorine (Cl) 8 atoms
Zinc (Zn) 8 atoms

Copper Binding Sites:

The binding sites of Copper atom in the The X-Ray Crystallographic Structure of Beta Carbonic Anhydrase From the C3 Dicot Pisum Sativum (pdb code 1ekj). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 4 binding sites of Copper where determined in the The X-Ray Crystallographic Structure of Beta Carbonic Anhydrase From the C3 Dicot Pisum Sativum, PDB code: 1ekj:
Jump to Copper binding site number: 1; 2; 3; 4;

Copper binding site 1 out of 4 in 1ekj

Go back to Copper Binding Sites List in 1ekj
Copper binding site 1 out of 4 in the The X-Ray Crystallographic Structure of Beta Carbonic Anhydrase From the C3 Dicot Pisum Sativum


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of The X-Ray Crystallographic Structure of Beta Carbonic Anhydrase From the C3 Dicot Pisum Sativum within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu4

b:73.5
occ:0.50
 O B:HOH1085 2.0 44.3 1.0
SG A:CYS166 2.5 71.4 1.0
SG B:CYS166 2.7 82.0 1.0
CD B:PRO167 3.4 83.7 1.0
CG B:PRO167 3.5 83.6 1.0
NH1 B:ARG182 3.6 58.7 1.0
OG B:SER168 3.7 92.5 1.0
NH1 A:ARG182 3.8 46.8 1.0
N B:SER168 4.0 88.1 1.0
CB B:SER168 4.0 90.8 1.0
OG A:SER168 4.1 75.0 1.0
CB B:CYS166 4.1 80.5 1.0
N B:PRO167 4.1 83.0 1.0
CB A:CYS166 4.2 65.8 1.0
CD A:PRO167 4.4 70.2 1.0
CA B:CYS166 4.4 80.4 1.0
CB A:SER168 4.4 74.0 1.0
CD B:ARG182 4.5 58.5 1.0
C B:CYS166 4.5 81.4 1.0
CG A:PRO167 4.6 70.1 1.0
CZ B:ARG182 4.6 58.8 1.0
CA B:SER168 4.7 89.9 1.0
CD A:ARG182 4.7 46.9 1.0
CA A:CYS166 4.8 65.5 1.0
N A:SER168 4.8 72.4 1.0
CB B:PRO167 4.8 84.0 1.0
CA B:PRO167 4.9 84.5 1.0
CZ A:ARG182 5.0 45.3 1.0
C B:PRO167 5.0 86.2 1.0

Copper binding site 2 out of 4 in 1ekj

Go back to Copper Binding Sites List in 1ekj
Copper binding site 2 out of 4 in the The X-Ray Crystallographic Structure of Beta Carbonic Anhydrase From the C3 Dicot Pisum Sativum


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of The X-Ray Crystallographic Structure of Beta Carbonic Anhydrase From the C3 Dicot Pisum Sativum within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cu1

b:51.9
occ:0.50
 SG D:CYS166 2.4 32.0 0.5
SG C:CYS166 2.5 35.5 0.5
NH1 C:ARG182 3.5 23.9 1.0
OG C:SER168 3.6 56.8 1.0
NH1 D:ARG182 3.7 23.6 1.0
OG D:SER168 3.8 49.5 1.0
CB C:CYS166 4.1 36.5 0.5
CB D:CYS166 4.1 31.3 0.5
CB D:SER168 4.1 46.6 1.0
CB C:CYS166 4.2 40.2 0.5
CB C:SER168 4.2 52.9 1.0
CD D:PRO167 4.3 33.5 1.0
N D:SER168 4.3 42.5 1.0
CG D:PRO167 4.4 35.3 1.0
CB D:CYS166 4.4 31.4 0.5
CD C:PRO167 4.5 42.2 1.0
CD D:ARG182 4.5 23.6 1.0
N C:SER168 4.5 49.1 1.0
CD C:ARG182 4.5 27.9 1.0
CG C:PRO167 4.5 44.1 1.0
N D:PRO167 4.6 34.6 1.0
CZ C:ARG182 4.6 21.4 1.0
CA C:CYS166 4.6 39.2 0.5
CA D:CYS166 4.7 31.8 0.5
CA C:CYS166 4.7 37.0 0.5
CA D:CYS166 4.7 32.0 0.5
N C:PRO167 4.8 41.8 1.0
CZ D:ARG182 4.8 27.6 1.0
CA D:SER168 4.8 45.0 1.0
C D:CYS166 4.8 33.5 0.5
C D:CYS166 4.9 33.4 0.5
C C:CYS166 4.9 40.7 0.5
C C:CYS166 4.9 39.6 0.5

Copper binding site 3 out of 4 in 1ekj

Go back to Copper Binding Sites List in 1ekj
Copper binding site 3 out of 4 in the The X-Ray Crystallographic Structure of Beta Carbonic Anhydrase From the C3 Dicot Pisum Sativum


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of The X-Ray Crystallographic Structure of Beta Carbonic Anhydrase From the C3 Dicot Pisum Sativum within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Cu3

b:53.4
occ:0.50
 O E:HOH1302 2.0 51.4 1.0
SG F:CYS166 2.3 67.9 1.0
SG E:CYS166 2.3 63.9 1.0
NH1 F:ARG182 3.7 39.5 1.0
OG E:SER168 3.7 62.3 1.0
CB E:SER168 3.8 61.0 1.0
CB F:CYS166 3.8 62.0 1.0
OG F:SER168 3.9 65.1 1.0
NH1 E:ARG182 3.9 38.1 1.0
CB F:SER168 3.9 63.1 1.0
CB E:CYS166 4.1 54.6 1.0
N E:SER168 4.2 59.8 1.0
CD F:PRO167 4.3 59.7 1.0
N F:SER168 4.3 60.7 1.0
CD E:PRO167 4.4 56.7 1.0
CG E:PRO167 4.4 57.3 1.0
CG F:PRO167 4.4 59.2 1.0
CA F:CYS166 4.4 60.1 1.0
N F:PRO167 4.6 60.0 1.0
CA E:SER168 4.7 61.3 1.0
N E:PRO167 4.7 55.7 1.0
CA E:CYS166 4.7 53.5 1.0
C F:CYS166 4.7 60.2 1.0
CD F:ARG182 4.8 40.4 1.0
CA F:SER168 4.8 62.7 1.0
CZ F:ARG182 4.8 37.6 1.0
CD E:ARG182 4.9 39.0 1.0
C E:CYS166 4.9 54.8 1.0

Copper binding site 4 out of 4 in 1ekj

Go back to Copper Binding Sites List in 1ekj
Copper binding site 4 out of 4 in the The X-Ray Crystallographic Structure of Beta Carbonic Anhydrase From the C3 Dicot Pisum Sativum


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of The X-Ray Crystallographic Structure of Beta Carbonic Anhydrase From the C3 Dicot Pisum Sativum within 5.0Å range:
probe atom residue distance (Å) B Occ
G:Cu2

b:50.0
occ:0.50
 O H:HOH1932 2.0 43.3 1.0
O G:HOH1935 2.0 40.7 1.0
OG G:SER168 2.8 52.9 1.0
SG H:CYS166 3.2 35.7 0.5
NH1 G:ARG182 3.4 28.1 1.0
CB G:SER168 3.5 49.1 1.0
CD G:PRO167 3.6 42.3 1.0
CB G:CYS166 3.7 45.8 0.5
N G:SER168 3.7 46.6 1.0
CB G:CYS166 3.7 45.2 0.5
CA G:CYS166 3.9 43.5 0.5
CA G:CYS166 3.9 43.8 0.5
CG G:PRO167 3.9 41.4 1.0
N G:PRO167 3.9 43.1 1.0
OG H:SER168 4.0 49.9 1.0
C G:CYS166 4.1 43.4 0.5
C G:CYS166 4.1 43.3 0.5
CA G:SER168 4.2 48.9 1.0
NH1 H:ARG182 4.3 24.3 1.0
SG G:CYS166 4.4 52.6 0.5
CB H:SER168 4.4 43.1 1.0
CD G:ARG182 4.5 30.3 1.0
CZ G:ARG182 4.5 28.7 1.0
CB H:CYS166 4.7 34.1 0.5
CB H:CYS166 4.7 32.7 0.5
C G:PRO167 4.7 44.9 1.0
CA G:PRO167 4.8 43.2 1.0
NE G:ARG182 4.9 27.9 1.0
O G:CYS166 5.0 43.0 0.5
O G:CYS166 5.0 42.9 0.5

Reference:

M.S.Kimber, E.F.Pai. The Active Site Architecture of Pisum Sativum Beta-Carbonic Anhydrase Is A Mirror Image of That of Alpha-Carbonic Anhydrases. Embo J. V. 19 1407 2000.
ISSN: ISSN 0261-4189
PubMed: 10747009
DOI: 10.1093/EMBOJ/19.7.1407
Page generated: Tue Jul 30 21:44:48 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy