Copper in PDB 1e30: Crystal Structure of the MET148GLN Mutant of Rusticyanin at 1.5 Angstrom Resolution

The structure of Crystal Structure of the MET148GLN Mutant of Rusticyanin at 1.5 Angstrom Resolution, PDB code: 1e30 was solved by M.A.Hough, R.W.Strange, S.S.Hasnain, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	52.70 / 1.50
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	42.861, 61.380, 53.330, 90.00, 96.19, 90.00
R / Rfree (%)	18.2 / 21.7

The binding sites of Copper atom in the Crystal Structure of the MET148GLN Mutant of Rusticyanin at 1.5 Angstrom Resolution (pdb code 1e30). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 2 binding sites of Copper where determined in the Crystal Structure of the MET148GLN Mutant of Rusticyanin at 1.5 Angstrom Resolution, PDB code: 1e30:
Jump to Copper binding site number: 1; 2;

Copper binding site 1 out of 2 in the Crystal Structure of the MET148GLN Mutant of Rusticyanin at 1.5 Angstrom Resolution


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Crystal Structure of the MET148GLN Mutant of Rusticyanin at 1.5 Angstrom Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Cu156 b:7.9 occ:1.00 ND1 A:HIS143 2.0 9.3 1.0 ND1 A:HIS85 2.0 6.8 1.0 SG A:CYS138 2.2 5.7 1.0 OE1 A:GLN148 2.3 7.9 1.0 CE1 A:HIS85 2.9 6.8 1.0 CE1 A:HIS143 3.0 11.9 1.0 CG A:HIS143 3.0 8.9 1.0 CG A:HIS85 3.1 5.9 1.0 CD A:GLN148 3.2 8.9 1.0 CB A:CYS138 3.2 6.2 1.0 CB A:HIS143 3.4 7.9 1.0 NE2 A:GLN148 3.5 10.0 1.0 CB A:HIS85 3.5 6.0 1.0 CA A:HIS85 3.6 6.6 1.0 O A:GLY84 3.9 10.9 1.0 CB A:ILE140 3.9 11.5 1.0 CG1 A:ILE140 4.0 13.6 1.0 NE2 A:HIS85 4.1 6.2 1.0 NE2 A:HIS143 4.1 10.8 1.0 CD2 A:HIS143 4.1 11.0 1.0 CD2 A:HIS85 4.2 6.5 1.0 N A:SER86 4.4 6.0 1.0 CG A:GLN148 4.5 6.7 1.0 CA A:CYS138 4.6 5.2 1.0 C A:HIS85 4.6 6.3 1.0 N A:HIS85 4.7 7.1 1.0 C A:GLY84 4.8 10.5 1.0 N A:ILE140 4.8 7.8 1.0 CG2 A:ILE140 4.8 13.4 1.0 CA A:HIS143 4.9 7.0 1.0 CB A:GLN148 4.9 5.7 1.0 CB A:PRO52 4.9 9.1 1.0 CA A:ILE140 5.0 9.4 1.0

Copper binding site 2 out of 2 in the Crystal Structure of the MET148GLN Mutant of Rusticyanin at 1.5 Angstrom Resolution


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Crystal Structure of the MET148GLN Mutant of Rusticyanin at 1.5 Angstrom Resolution within 5.0Å range: probe atom residue distance (Å) B Occ B:Cu156 b:6.9 occ:1.00 ND1 B:HIS85 2.0 4.6 1.0 ND1 B:HIS143 2.0 8.6 1.0 SG B:CYS138 2.2 5.6 1.0 OE1 B:GLN148 2.4 5.9 1.0 CE1 B:HIS85 2.9 6.2 1.0 CE1 B:HIS143 3.0 9.7 1.0 CG B:HIS143 3.0 8.2 1.0 CG B:HIS85 3.1 5.7 1.0 CB B:CYS138 3.1 7.4 1.0 CD B:GLN148 3.3 6.2 1.0 CB B:HIS143 3.4 6.3 1.0 NE2 B:GLN148 3.5 8.6 1.0 CB B:HIS85 3.6 5.3 1.0 CD1 B:ILE140 3.7 8.1 1.0 CA B:HIS85 3.8 5.3 1.0 CB B:ILE140 3.9 7.6 1.0 CG1 B:ILE140 3.9 7.8 1.0 NE2 B:HIS85 4.1 6.3 1.0 NE2 B:HIS143 4.2 9.6 1.0 CD2 B:HIS143 4.2 8.6 1.0 CD2 B:HIS85 4.2 5.3 1.0 CA B:CYS138 4.6 5.9 1.0 CG B:GLN148 4.6 5.2 1.0 N B:SER86 4.6 4.9 1.0 N B:ILE140 4.7 7.6 1.0 N B:HIS85 4.7 5.7 1.0 C B:HIS85 4.8 5.0 1.0 CG2 B:ILE140 4.8 8.5 1.0 CA B:HIS143 4.9 5.5 1.0 CA B:ILE140 4.9 7.8 1.0 CB B:GLN148 4.9 5.0 1.0

J.F.Hall, L.D.Kanbi, R.W.Strange, S.S.Hasnain. Role of the Axial Ligand in Type 1 Cu Centers Studied By Point Mutations of MET148 in Rusticyanin Biochemistry V. 38 12675 1999.
ISSN: ISSN 0006-2960
PubMed: 10504237
DOI: 10.1021/BI990983G