Atomistry » Copper » PDB 1a2v-1baw » 1aq8
Atomistry »
  Copper »
    PDB 1a2v-1baw »
      1aq8 »

Copper in PDB 1aq8: Structure of Alcaligenes Faecalis Nitrite Reductase Reduced with Ascorbate

Enzymatic activity of Structure of Alcaligenes Faecalis Nitrite Reductase Reduced with Ascorbate

All present enzymatic activity of Structure of Alcaligenes Faecalis Nitrite Reductase Reduced with Ascorbate:
1.7.99.3;

Protein crystallography data

The structure of Structure of Alcaligenes Faecalis Nitrite Reductase Reduced with Ascorbate, PDB code: 1aq8 was solved by M.E.P.Murphy, E.T.Adman, S.Turley, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 10.00 / 2.00
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 61.790, 102.400, 146.200, 90.00, 90.00, 90.00
R / Rfree (%) 18.9 / n/a

Copper Binding Sites:

The binding sites of Copper atom in the Structure of Alcaligenes Faecalis Nitrite Reductase Reduced with Ascorbate (pdb code 1aq8). This binding sites where shown within 5.0 Angstroms radius around Copper atom.
In total 6 binding sites of Copper where determined in the Structure of Alcaligenes Faecalis Nitrite Reductase Reduced with Ascorbate, PDB code: 1aq8:
Jump to Copper binding site number: 1; 2; 3; 4; 5; 6;

Copper binding site 1 out of 6 in 1aq8

Go back to Copper Binding Sites List in 1aq8
Copper binding site 1 out of 6 in the Structure of Alcaligenes Faecalis Nitrite Reductase Reduced with Ascorbate


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 1 of Structure of Alcaligenes Faecalis Nitrite Reductase Reduced with Ascorbate within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu501

b:17.8
occ:1.00
ND1 A:HIS95 2.0 11.6 1.0
ND1 A:HIS145 2.1 11.9 1.0
SG A:CYS136 2.1 15.1 1.0
SD A:MET150 2.6 18.0 1.0
CE1 A:HIS95 3.0 7.0 1.0
CE1 A:HIS145 3.1 11.7 1.0
CB A:CYS136 3.1 11.6 1.0
CG A:HIS95 3.1 7.2 1.0
CG A:HIS145 3.2 13.3 1.0
CE A:MET150 3.5 11.4 1.0
CB A:HIS145 3.5 10.6 1.0
CB A:HIS95 3.5 10.0 1.0
CG A:MET150 3.8 13.1 1.0
CA A:HIS95 4.0 10.8 1.0
H A:ASN96 4.1 0.0 1.0
NE2 A:HIS95 4.1 3.2 1.0
CG A:PRO138 4.2 14.8 1.0
CD2 A:HIS95 4.2 10.7 1.0
NE2 A:HIS145 4.2 10.8 1.0
CD2 A:HIS145 4.3 10.7 1.0
O A:MET94 4.3 5.7 1.0
SD A:MET62 4.3 16.2 1.0
CB A:MET150 4.4 8.6 1.0
CD A:PRO138 4.4 16.6 1.0
CA A:CYS136 4.5 14.0 1.0
CA A:HIS145 4.7 12.2 1.0
CB A:MET62 4.7 13.9 1.0
N A:ASN96 4.9 14.0 1.0
N A:HIS95 5.0 12.8 1.0
HE2 A:HIS95 5.0 0.0 1.0

Copper binding site 2 out of 6 in 1aq8

Go back to Copper Binding Sites List in 1aq8
Copper binding site 2 out of 6 in the Structure of Alcaligenes Faecalis Nitrite Reductase Reduced with Ascorbate


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 2 of Structure of Alcaligenes Faecalis Nitrite Reductase Reduced with Ascorbate within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cu502

b:16.9
occ:1.00
NE2 A:HIS100 1.9 13.8 1.0
NE2 A:HIS135 1.9 12.2 1.0
NE2 B:HIS306 2.0 16.7 1.0
CE1 A:HIS100 2.7 15.2 1.0
CE1 B:HIS306 2.8 10.9 1.0
CE1 A:HIS135 2.9 9.3 1.0
CD2 A:HIS135 3.0 14.6 1.0
CD2 A:HIS100 3.1 11.3 1.0
CD2 B:HIS306 3.3 12.6 1.0
OD1 A:ASP98 3.6 24.6 1.0
ND1 A:HIS100 4.0 11.9 1.0
ND1 B:HIS306 4.0 18.7 1.0
HE2 B:HIS255 4.1 0.0 1.0
ND1 A:HIS135 4.1 16.6 1.0
CG A:HIS135 4.1 9.9 1.0
NE2 B:HIS255 4.1 8.8 1.0
CG A:HIS100 4.2 16.2 1.0
CG B:HIS306 4.3 14.0 1.0
CG A:ASP98 4.3 19.0 1.0
CE1 B:HIS255 4.5 12.0 1.0
CD2 B:HIS255 4.5 10.8 1.0
H1 B:HOH2104 4.7 0.0 1.0
HD1 A:HIS100 4.8 0.0 1.0
HD1 B:HIS306 4.8 0.0 1.0
OD2 A:ASP98 4.9 18.0 1.0
HD1 A:HIS135 5.0 0.0 1.0
CG1 B:ILE257 5.0 23.7 1.0
ND1 B:HIS255 5.0 13.3 1.0
CD2 B:LEU308 5.0 8.8 1.0

Copper binding site 3 out of 6 in 1aq8

Go back to Copper Binding Sites List in 1aq8
Copper binding site 3 out of 6 in the Structure of Alcaligenes Faecalis Nitrite Reductase Reduced with Ascorbate


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 3 of Structure of Alcaligenes Faecalis Nitrite Reductase Reduced with Ascorbate within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu501

b:22.2
occ:1.00
ND1 B:HIS95 2.1 20.6 1.0
ND1 B:HIS145 2.1 17.4 1.0
SG B:CYS136 2.2 18.2 1.0
SD B:MET150 2.6 17.8 1.0
CE1 B:HIS145 2.9 20.8 1.0
CG B:HIS95 3.1 24.2 1.0
CE1 B:HIS95 3.1 22.0 1.0
CB B:CYS136 3.1 16.3 1.0
CG B:HIS145 3.3 17.7 1.0
CE B:MET150 3.3 21.1 1.0
CB B:HIS95 3.4 24.0 1.0
CB B:HIS145 3.7 17.5 1.0
CA B:HIS95 3.9 22.1 1.0
H B:ASN96 4.0 0.0 1.0
CG B:MET150 4.0 13.8 1.0
CG B:PRO138 4.0 21.7 1.0
NE2 B:HIS145 4.1 17.1 1.0
NE2 B:HIS95 4.2 24.2 1.0
CD2 B:HIS95 4.2 19.3 1.0
O B:MET94 4.3 20.9 1.0
CD2 B:HIS145 4.3 21.3 1.0
SD B:MET62 4.3 16.9 1.0
CD B:PRO138 4.4 19.6 1.0
CB B:MET150 4.5 10.3 1.0
CA B:CYS136 4.5 17.4 1.0
N B:ASN96 4.7 21.8 1.0
CB B:MET62 4.8 19.0 1.0
N B:HIS95 4.8 22.8 1.0
C B:HIS95 4.9 23.1 1.0
C B:MET94 4.9 23.1 1.0
CA B:HIS145 4.9 19.3 1.0
HE2 B:HIS145 5.0 0.0 1.0
O B:ASN96 5.0 21.8 1.0

Copper binding site 4 out of 6 in 1aq8

Go back to Copper Binding Sites List in 1aq8
Copper binding site 4 out of 6 in the Structure of Alcaligenes Faecalis Nitrite Reductase Reduced with Ascorbate


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 4 of Structure of Alcaligenes Faecalis Nitrite Reductase Reduced with Ascorbate within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cu502

b:19.4
occ:1.00
NE2 B:HIS100 2.0 11.1 1.0
NE2 B:HIS135 2.0 15.6 1.0
NE2 C:HIS306 2.1 12.2 1.0
CE1 C:HIS306 2.8 13.8 1.0
CE1 B:HIS100 2.8 11.9 1.0
CE1 B:HIS135 3.0 17.4 1.0
CD2 B:HIS135 3.0 15.0 1.0
CD2 B:HIS100 3.2 15.6 1.0
CD2 C:HIS306 3.3 15.7 1.0
OD1 B:ASP98 3.5 26.8 1.0
CD1 C:ILE257 3.8 24.4 1.0
H1 B:HOH1918 3.8 0.0 1.0
HE2 C:HIS255 4.0 0.0 1.0
ND1 B:HIS100 4.0 12.7 1.0
ND1 C:HIS306 4.1 14.0 1.0
NE2 C:HIS255 4.1 24.9 1.0
ND1 B:HIS135 4.1 14.0 1.0
CG B:HIS135 4.2 16.8 1.0
CG B:HIS100 4.2 12.0 1.0
CG B:ASP98 4.3 20.6 1.0
CG C:HIS306 4.3 14.6 1.0
CE1 C:HIS255 4.4 24.2 1.0
CD2 C:HIS255 4.5 21.7 1.0
H1 C:HOH2740 4.6 0.0 1.0
O B:HOH1918 4.7 36.3 1.0
O C:HOH2740 4.7 23.9 1.0
HD1 B:HIS100 4.8 0.0 1.0
HD1 C:HIS306 4.9 0.0 1.0
OD2 B:ASP98 4.9 14.0 1.0
ND1 C:HIS255 5.0 21.8 1.0
CD2 C:LEU308 5.0 22.3 1.0

Copper binding site 5 out of 6 in 1aq8

Go back to Copper Binding Sites List in 1aq8
Copper binding site 5 out of 6 in the Structure of Alcaligenes Faecalis Nitrite Reductase Reduced with Ascorbate


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 5 of Structure of Alcaligenes Faecalis Nitrite Reductase Reduced with Ascorbate within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cu501

b:23.8
occ:1.00
ND1 C:HIS145 2.0 25.0 1.0
ND1 C:HIS95 2.1 25.0 1.0
SG C:CYS136 2.2 21.5 1.0
SD C:MET150 2.6 19.6 1.0
CE1 C:HIS145 2.9 21.3 1.0
CE1 C:HIS95 3.0 21.3 1.0
CG C:HIS95 3.1 19.4 1.0
CG C:HIS145 3.2 22.5 1.0
CB C:CYS136 3.2 21.2 1.0
CB C:HIS95 3.5 14.7 1.0
CE C:MET150 3.6 16.3 1.0
CB C:HIS145 3.6 19.7 1.0
CA C:HIS95 3.9 18.1 1.0
CG C:MET150 4.0 17.6 1.0
H C:ASN96 4.0 0.0 1.0
CG C:PRO138 4.0 16.5 1.0
NE2 C:HIS145 4.1 21.5 1.0
SD C:MET62 4.2 17.4 1.0
NE2 C:HIS95 4.2 17.9 1.0
O C:MET94 4.2 16.6 1.0
CD2 C:HIS95 4.2 20.7 1.0
CD2 C:HIS145 4.3 20.5 1.0
CD C:PRO138 4.5 15.2 1.0
CB C:MET150 4.5 18.1 1.0
CA C:CYS136 4.6 20.7 1.0
CB C:MET62 4.7 17.6 1.0
N C:ASN96 4.8 19.7 1.0
CA C:HIS145 4.8 19.5 1.0
N C:HIS95 4.9 19.0 1.0
C C:MET94 4.9 20.8 1.0
C C:HIS95 4.9 17.8 1.0
HE2 C:HIS145 5.0 0.0 1.0

Copper binding site 6 out of 6 in 1aq8

Go back to Copper Binding Sites List in 1aq8
Copper binding site 6 out of 6 in the Structure of Alcaligenes Faecalis Nitrite Reductase Reduced with Ascorbate


Mono view


Stereo pair view

A full contact list of Copper with other atoms in the Cu binding site number 6 of Structure of Alcaligenes Faecalis Nitrite Reductase Reduced with Ascorbate within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Cu502

b:19.9
occ:1.00
NE2 C:HIS100 1.9 11.0 1.0
NE2 A:HIS306 2.1 15.3 1.0
NE2 C:HIS135 2.1 18.0 1.0
CE1 C:HIS100 2.7 14.1 1.0
CE1 A:HIS306 2.8 11.3 1.0
CE1 C:HIS135 3.1 16.8 1.0
CD2 C:HIS100 3.1 15.2 1.0
CD2 C:HIS135 3.1 21.1 1.0
CD2 A:HIS306 3.3 15.5 1.0
CD1 A:ILE257 3.6 25.5 1.0
OD1 C:ASP98 3.7 26.0 1.0
HE2 A:HIS255 3.9 0.0 1.0
H2 C:HOH2530 3.9 0.0 1.0
ND1 C:HIS100 3.9 14.4 1.0
ND1 A:HIS306 4.0 15.3 1.0
NE2 A:HIS255 4.0 24.3 1.0
CG C:HIS100 4.1 13.9 1.0
ND1 C:HIS135 4.2 18.4 1.0
CG C:HIS135 4.3 21.5 1.0
CG A:HIS306 4.3 15.9 1.0
CG C:ASP98 4.4 22.0 1.0
CE1 A:HIS255 4.4 26.5 1.0
CD2 A:HIS255 4.4 23.5 1.0
HD1 C:HIS100 4.7 0.0 1.0
HD1 A:HIS306 4.8 0.0 1.0
O C:HOH2530 4.8 41.2 1.0
OD2 C:ASP98 4.9 21.5 1.0
O A:HOH1369 4.9 11.5 1.0
ND1 A:HIS255 4.9 25.6 1.0
CG A:HIS255 5.0 24.7 1.0

Reference:

M.E.Murphy, S.Turley, E.T.Adman. Structure of Nitrite Bound to Copper-Containing Nitrite Reductase From Alcaligenes Faecalis. Mechanistic Implications. J.Biol.Chem. V. 272 28455 1997.
ISSN: ISSN 0021-9258
PubMed: 9353305
DOI: 10.1074/JBC.272.45.28455
Page generated: Sun Dec 13 10:57:43 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy